pLV3-CMV-UFM1-3×FLAG-CopGFP-Pu

pLV3-CMV-UFM1-3×FLAG-CopGFP-Puro plasmid

PVT108824

pLV3-CMV-UFM1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-UFM1(human)-3×FLAG-CopGFP-Puro
Alias: NM_016617.4;BM-002; C13orf20; HLD14
Gene lenght: 255
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-UFM1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-UFM1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-UFM1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgt cgaaggtttc ctttaagatc 2341 acgctgacgt cggacccacg gctgccgtac aaagtactca gtgttcctga aagtacacct 2401 ttcacagcag tcttaaagtt tgcagcagaa gaatttaaag ttcctgctgc aacaagtgca 2461 attattacca atgatggaat aggaataaat cctgcacaga ctgctggaaa tgtttttcta 2521 aaacatggtt cagaactgcg gattattcct agagatcgtg ttggaagttg tggatccGAC 2581 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC 2641 AAGtagcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca 2701 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga 2761 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 2821 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 2881 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc 2941 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt 3001 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc 3061 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg 3121 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc 3181 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc 3241 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg 3301 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa 3361 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac 3421 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc 3481 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc 3541 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc 3601 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg 3661 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac 3721 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc 3781 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc 3841 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc 3901 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc 3961 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc 4021 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 4081 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 4141 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 4201 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 4261 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 4321 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 4381 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 4441 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 4501 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 4561 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 4621 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 4681 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 4741 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 4801 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 4861 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 4921 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 4981 ctgttgggca ctgacaattc cgtggtgttg tcggggaaAT CATCGTCCTT TCCTtggctg 5041 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 5101 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 5161 ctTcgccttc gccctcagac gagtcggatc tccctttggG ccgcctcccc gcctggtacc 5221 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 5281 gggactggaa gggctaattc actcccaacg aaGataagat ctgctttttg cttgtactgg 5341 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 5401 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 5461 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 5521 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 5581 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 5641 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 5701 caatgtatct tatcatgtct ggctctagct aTCCCGCCCC TAACTCCGCC CAtcccgccc 5761 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 5821 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 5881 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg 5941 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 6001 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 6061 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 6121 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 6181 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 6241 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 6301 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 6361 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 6421 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 6481 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 6541 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 6601 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 6661 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 6721 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 6781 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 6841 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 6901 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 6961 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 7021 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 7081 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 7141 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 7201 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 7261 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 7321 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 7381 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 7441 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 7501 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 7561 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 7621 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 7681 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 7741 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 7801 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 7861 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 7921 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 7981 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 8041 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 8101 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 8161 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 8221 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 8281 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 8341 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg 8401 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 8461 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 8521 taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.