pLV2-TRE3GS-MCS-killerred-TetO

pLV2-TRE3GS-MCS-killerred-TetOne-Puro plasmid

PVT100010

pLV2-TRE3GS-MCS-killerred-TetOne-Puro plasmid Information

Plasmid name: pLV2-TRE3GS-MCS-killerred-TetOne-Puro
Alias: -
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression,Tetracycline induction
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: TRE3G
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CPPT-F
Reverse primer:
Copy number: High
Induction: Tetracycline induction

pLV2-TRE3GS-MCS-killerred-TetOne-Puro plasmid Description

pLV2-TRE3GS-MCS-killerred-TetOne-Puro is a plasmid suitable for protein expression in mammalian cells, tetracycline-inducible, and can also be used for lentivirus packaging.

pLV2-TRE3GS-MCS-killerred-TetOne-Puro plasmid Sequence

1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc 4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc 4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc 4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg 4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg 4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc 4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac 4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc 4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg 4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg 4681 tactcggtca tggtcgcgtt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact 4741 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc 4801 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta 4861 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 4921 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac 4981 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt 5041 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg 5101 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg 5161 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg 5221 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga 5281 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc 5341 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg 5401 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac 5461 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga 5521 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg 5581 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc 5641 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt 5701 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg 5761 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg 5821 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg 5881 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc 5941 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc 6001 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg 6061 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga 6121 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg 6181 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc 6241 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg 6301 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac 6361 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca 6421 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt 6481 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac 6541 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc 6601 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt 6661 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcatgtt cgaaACCGGT 6721 ATGCTGTGCT GTATGAGAAG AACCAAACAG GTTGAAAAGA ATGATGAGGA CCAAAAGATC 6781 TCCGAGGGCG GCCCCGCCCT GTTCCAGAGC GACATGACCT TCAAAATCTT CATCGACGGC 6841 GAGGTGAACG GCCAGAAGTT CACCATCGTG GCCGACGGCA GCAGCAAGTT CCCCCACGGC 6901 GACTTCAACG TGCACGCCGT GTGCGAGACC GGCAAGCTGC CCATGAGCTG GAAGCCCATC 6961 TGCCACCTGA TCCAGTACGG CGAGCCCTTC TTCGCCCGCT ACCCCGACGG CATCAGCCAT 7021 TTCGCCCAGG AGTGCTTCCC CGAGGGCCTG AGCATCGACC GCACCGTGCG CTTCGAGAAC 7081 GACGGCACCA TGACCAGCCA CCACACCTAC GAGCTGGACG ACACCTGCGT GGTGAGCCGC 7141 ATCACCGTGA ACTGCGACGG CTTCCAGCCC GACGGCCCCA TCATGCGCGA CCAGCTGGTG 7201 GACATCCTGC CCAACGAGAC CCACATGTTC CCCCACGGCC CCAACGCCGT GCGCCAGCTG 7261 GCCTTCATCG GCTTCACCAC CGCCGACGGC GGCCTGATGA TGGGCCACTT CGACAGCAAG 7321 ATGACCTTCA ACGGCAGCCG CGCCATCGAG ATCCCCGGCC CACACTTCGT GACCATCATC 7381 ACCAAGCAGA TGAGGGACAC CAGCGACAAG CGCGACCACG TGTGCCAGCG CGAGGTGGCC 7441 TACGCCCACA GCGTGCCCCG CATCACCAGC GCCATCGGTA GCGACGAGGA TTAAggatcc 7501 agaccacctc ccctgcgagc taagctggac agccaatgac gggtaagaga gtgacatttt 7561 tcactaacct aagacaggag ggccgtcaga gctactgcct aatccaaaga cgggtaaaag 7621 tgataaaaat gtatcactcc aacctaagac aggcgcagct tccgagggat ttgagatcca 7681 gacatgataa gatacattga tgagtttgga caaaccaaaa ctagaatgca gtgaaaaaaa 7741 tgccttattt gtgaaatttg tgatgctatt gccttatttg taaccattat aagctgcaat 7801 aaacaagtta agtcgataaa ctggatctct gctgtccctg taataaaccc gaaaattttg 7861 aatttttgta atttgttttt gtagttcttt agtttgtatg tctgttgcta ttatgtctac 7921 tattctttcc cctgcactgt accccccaat cccccctttt cttttaaagg cgataccgtc 7981 gagatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta 8041 ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg 8101 aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta 8161 ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa 8221 accaattcca caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt 8281 gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc 8341 tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac 8401 tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca 8461 ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga 8521 tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt 8581 tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg 8641 ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca 8701 ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg 8761 gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc 8821 ctcctccagg tctgaagatc agcggccggc cgcttgctgt gcggtggtct tacttttgtt 8881 ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg 8941 cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg 9001 aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc 9061 aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata 9121 ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg 9181 cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt 9241 tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt 9301 cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca 9361 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca 9421 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc 9481 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac 9541 ccagtacagg caaaaagcag ctgcttatat gcaggatctg agggctcgcc actccccagt 9601 cccgcccagg ccacgcctcc ctggaaagtc cccagcggaa agtcccttgt agcaagctcg 9661 atatcagcag ttcttgaagt actccggatg cagctctcgg gccacgtgat gaaatgctag 9721 gcggctgtca aacctccact ctaacacttc tctctccggg tcatccatcc catgcaggct 9781 cacagggtgt aacaagctgg tgttctctcc tttattggcc tcttctacct tatctggctc 9841 aactggtact agcttgtagc accatccaaa ggtcagtgga tatctgaccc ctggccctgg 9901 tgtgtagttc tgctaatcag ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat 9961 atcttgtctt ctttgggagt gaattagccc ttcca

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.