pLV2-EF1a-Klf2-IRES-EGFP-PGK-P

pLV2-EF1a-Klf2-IRES-EGFP-PGK-Puro plasmid

PVT111274

pLV2-EF1a-Klf2-IRES-EGFP-PGK-Puro plasmid Information

Plasmid name: pLV2-EF1a-Klf2(mouse)-IRES-EGFP-PGK-Puro
Alias: NM_008452.2;Lklf
Gene lenght: 1065
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF-1α promoter-F1
Reverse primer:
Copy number: High
Induction:

pLV2-EF1a-Klf2-IRES-EGFP-PGK-Puro plasmid Description

pLV2-EF1a-Klf2-IRES-EGFP-PGK-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV2-EF1a-Klf2-IRES-EGFP-PGK-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgat tcgaattcGC CACCatggcg 3541 ctcagcgagc ctatcttgcc gtcctttgcc actttcgcca gcccgtgcga gcgcggcctc 3601 caggagcgct ggccgcgaaa tgaacccgag gcgggcggca cggatgagga cctaaacaac 3661 gtgttggact tcatcctctc catgggattg gacggtctgg gcgccgaaaa tcctcccgag 3721 cccccgccgc agcccccgcc gcctgccttc tactacccgg agccgggtgc tccgccgccc 3781 tacagcatcc ccgcggccag cctgggaaca gagctgctgc gccccgacct ggacccgcct 3841 caggggccgg ctctgcacgg ccgcttcctc ctcgcgcctc ccgggcggct agtgaaggcc 3901 gagccccccg aggtggacgg cggcggctac ggctgcgctc cgggcctggc ccacggaccg 3961 cgcgggctga agctcgaggg cgccccagga gcgacaggtg catgcatgcg gggtcccgcc 4021 ggccgccccc cgccgccccc ggacacgccg ccgctcagcc ccgacggccc cctgcgcatc 4081 ccggcgtccg gtccccgcaa cccgttcccg ccgcccttcg gtcccggccc cagcttcggc 4141 ggtcccggcc ccgcgttgca ctacgggcct cccgcgcctg gcgccttcgg tcttttcgag 4201 gacgcggcgg cagcggcggc ggcgctgggc ttggctccac ctgccacgcg cggtctcctc 4261 acgccgccct cgtccccgct ggagctgctg gaggccaagc ccaaacgcgg ccgccgctcc 4321 tggccccgca agcgcgccgc cacacatact tgcagctaca ccaactgcgg caagacctac 4381 accaagagct cgcacctaaa ggcgcatctg cgtacacaca caggtgagaa gccttatcat 4441 tgcaactggg aaggatgcgg ctggaagttc gcgcgctctg acgagcttac ccgccactac 4501 cgaaagcaca caggtcacag accctttcag tgccacttgt gcgaccgggc cttctccagg 4561 tccgaccacc tggccttgca catgaagcga cacatgtagg gatcccgccc ctctccctcc 4621 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat 4681 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct 4741 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg 4801 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta 4861 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag 4921 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg 4981 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat 5041 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca 5101 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc 5161 ctttgaaaaa cacgatgata agcttgccac aacccATGGT GAGCAAGGGC GAGGAGCTGT 5221 TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA 5281 GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT 5341 GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG 5401 TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA 5461 TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA 5521 CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA 5581 TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC 5641 ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC 5701 GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA 5761 TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA 5821 GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG 5881 GGATCACTCT CGGCATGGAC GAGCTGTACA AGtagtctag ataattctac cgggtagggg 5941 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc 6001 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg 6061 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc 6121 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta 6181 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg 6241 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg 6301 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc 6361 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc 6421 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct 6481 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta 6541 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca 6601 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 6661 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 6721 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 6781 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 6841 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 6901 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 6961 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 7021 ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt 7081 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 7141 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 7201 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 7261 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 7321 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 7381 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 7441 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 7501 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 7561 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 7621 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct 7681 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg 7741 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag 7801 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact 7861 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac 7921 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact 7981 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa 8041 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga 8101 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga 8161 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc 8221 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc 8281 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag 8341 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 8401 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 8461 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc 8521 agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc 8581 gttttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 8641 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 8701 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 8761 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 8821 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 8881 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 8941 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 9001 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 9061 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 9121 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 9181 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 9241 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 9301 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 9361 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 9421 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 9481 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 9541 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 9601 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 9661 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 9721 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 9781 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 9841 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 9901 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 9961 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 10021 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 10081 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 10141 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 10201 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 10261 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 10321 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 10381 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 10441 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 10501 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 10561 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 10621 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 10681 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 10741 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt 10801 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 10861 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 10921 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata 10981 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga 11041 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt 11101 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.