pLV2-EF1a-Klf4-IRES-EGFP-PGK-P

pLV2-EF1a-Klf4-IRES-EGFP-PGK-Puro plasmid

PVT111303

pLV2-EF1a-Klf4-IRES-EGFP-PGK-Puro plasmid Information

Plasmid name: pLV2-EF1a-Klf4(mouse)-IRES-EGFP-PGK-Puro
Alias: NM_010637.3;EZF; Gklf; Zie
Gene lenght: 1452
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pLV2-EF1a-Klf4-IRES-EGFP-PGK-Puro plasmid Description

pLV2-EF1a-Klf4-IRES-EGFP-PGK-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV2-EF1a-Klf4-IRES-EGFP-PGK-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgat tcgaattcGC CACCatgagg 3541 cagccacctg gcgagtctga catggctgtc agcgacgctc tgctcccgtc cttctccacg 3601 ttcgcgtccg gcccggcggg aagggagaag acactgcgtc cagcaggtgc cccgactaac 3661 cgttggcgtg aggaactctc tcacatgaag cgacttcccc cacttcccgg ccgcccctac 3721 gacctggcgg cgacggtggc cacagacctg gagagtggcg gagctggtgc agcttgcagc 3781 agtaacaacc cggccctcct agcccggagg gagaccgagg agttcaacga cctcctggac 3841 ctagacttta tcctttccaa ctcgctaacc caccaggaat cggtggccgc caccgtgacc 3901 acctcggcgt cagcttcatc ctcgtcttcc ccggcgagca gcggccctgc cagcgcgccc 3961 tccacctgca gcttcagcta tccgatccgg gccgggggtg acccgggcgt ggctgccagc 4021 aacacaggtg gagggctcct ctacagccga gaatctgcgc cacctcccac ggcccccttc 4081 aacctggcgg acatcaatga cgtgagcccc tcgggcggct tcgtggctga gctcctgcgg 4141 ccggagttgg acccagtata cattccgcca cagcagcctc agccgccagg tggcgggctg 4201 atgggcaagt ttgtgctgaa ggcgtctctg accacccctg gcagcgagta cagcagccct 4261 tcggtcatca gtgttagcaa aggaagccca gacggcagcc accccgtggt agtggcgccc 4321 tacagcggtg gcccgccgcg catgtgcccc aagattaagc aagaggcggt cccgtcctgc 4381 acggtcagcc ggtccctaga ggcccatttg agcgctggac cccagctcag caacggccac 4441 cggcccaaca cacacgactt ccccctgggg cggcagctcc ccaccaggac tacccctaca 4501 ctgagtcccg aggaactgct gaacagcagg gactgtcacc ctggcctgcc tcttccccca 4561 ggattccatc cccatccggg gcccaactac cctcctttcc tgccagacca gatgcagtca 4621 caagtcccct ctctccatta tcaagagctc atgccaccgg gttcctgcct gccagaggag 4681 cccaagccaa agaggggaag aaggtcgtgg ccccggaaaa gaacagccac ccacacttgt 4741 gactatgcag gctgtggcaa aacctatacc aagagttctc atctcaaggc acacctgcga 4801 actcacacag gcgagaaacc ttaccactgt gactgggacg gctgtgggtg gaaattcgcc 4861 cgctccgatg aactgaccag gcactaccgc aaacacacag ggcaccggcc ctttcagtgc 4921 cagaagtgtg acagggcctt ttccaggtcg gaccaccttg ccttacacat gaagaggcac 4981 ttttaaggat cccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg 5041 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc 5101 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc 5161 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa 5221 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct 5281 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca 5341 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca 5401 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat 5461 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc 5521 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac 5581 ccATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG 5641 ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT 5701 ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA 5761 CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA 5821 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT 5881 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC 5941 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC 6001 ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA 6061 ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG 6121 CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC 6181 ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG 6241 TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGt 6301 agtctagata attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg 6361 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt 6421 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt 6481 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt 6541 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat 6601 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg 6661 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg 6721 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg 6781 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat 6841 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 6901 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 6961 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 7021 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 7081 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 7141 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 7201 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 7261 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 7321 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 7381 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc 7441 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 7501 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 7561 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 7621 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 7681 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 7741 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 7801 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc 7861 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 7921 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 7981 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 8041 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac 8101 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt 8161 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 8221 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga 8281 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac 8341 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt 8401 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt 8461 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg 8521 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata 8581 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg 8641 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg 8701 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 8761 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 8821 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 8881 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 8941 agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg 9001 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 9061 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 9121 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 9181 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 9241 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 9301 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 9361 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 9421 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 9481 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 9541 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 9601 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 9661 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 9721 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 9781 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 9841 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 9901 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 9961 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 10021 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 10081 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 10141 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 10201 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 10261 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 10321 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 10381 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 10441 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 10501 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 10561 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 10621 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 10681 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 10741 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 10801 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 10861 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 10921 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 10981 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 11041 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 11101 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 11161 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag 11221 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 11281 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca 11341 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca 11401 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta 11461 aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca 11521 aacttagtag t

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.