pCMV-Itgav-3×FLAG-T2A-EGFP-Neo

pCMV-Itgav-3×FLAG-T2A-EGFP-Neo plasmid

PVT111034

pCMV-Itgav-3×FLAG-T2A-EGFP-Neo plasmid Information

Plasmid name: pCMV-Itgav(mouse)-3×FLAG-T2A-EGFP-Neo
Alias: NM_008402.4;1110004F14Rik; 2610028E01Rik; CD51;D430040G12Rik
Gene lenght: 3132
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Itgav-3×FLAG-T2A-EGFP-Neo plasmid Description

pCMV-Itgav-3×FLAG-T2A-EGFP-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Itgav-3×FLAG-T2A-EGFP-Neo plasmid Sequence

1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat 61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 781 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc 841 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag 901 cgtttaaact taagcttggt accGCCACCa tggctgctcc cgggcgcctg ctgctacgcc 961 ctcgccccgg cggcctcctg ctgctgctgc cgggtctcct gctgcccctc gccgacgcct 1021 tcaacctgga cgtcgaaagt cccgccgagt atgcgggtcc cgagggaagt tacttcggat 1081 tcgccgtgga cttcttcgag cccagcacgt cctccaggat gtttctcctg gtgggagccc 1141 ccaaagcgaa cacgacccag cctgggattg tagaaggagg gcaagttctc aaatgtgaat 1201 gctcatccag ccgccggtgc cagcccattg agtttgattc aacaggcaat cgagattatg 1261 ccaaagatga cccactggag tttaagtccc accagtggtt tggagcctct gtgaggtcaa 1321 agcaggataa aatcttggcc tgtgctccat tgtaccactg gaggactgag atgaagcagg 1381 agagagagcc agttggaacc tgctttcttc aggacggcac aaagaccgtt gagtatgctc 1441 catgcaggtc aaaaaatatt gatgctgatg gccagggatt ttgtcaagga ggattcagca 1501 ttgattttac taaagctgac agagtacttc tcggtggtcc tggtagcttt tattggcaag 1561 gtcagctcat ttcggaccaa gtggcagaaa tcatatctaa atatgaccca aatgtctaca 1621 gcatcaaata taataaccaa ttagcaacac ggactgcaca agcaattttt gatgacagtt 1681 atttgggtta ctctgtggcc gtgggagact tcaatggtga cggcattgaa gattttgtct 1741 caggagttcc aagagcagca aggactttgg gaatggttta tatttatgat gggaaaaata 1801 tgtcctcttt acacaatttt actggtgaac agatggctgc gtattttgga ttttctgtag 1861 ctgctactga cattaatggg gatgattacg cagatgtgtt tattggagcc cccctgttca 1921 tggaccgagg ttccgatggg aaactccagg aggttggcca ggtctcagtg tctctgcaga 1981 gagcagtggg agacttccag actacaaagc tgaacggctt tgaggttttt gccaggtttg 2041 gaagtgccat agctccattg ggagacctgg accaggatgg cttcaatgat attgcaattg 2101 ctgctcccta tggtggtgaa gataagaagg gacttgttta tatcttcaat ggaaggtcca 2161 caggcttgaa ttcggtgcca tctcaaatcc tcgaggggca gtgggctgct cagagcatgc 2221 ccccaagctt tggctattca atgaagggag ctacagatgt agacaggaat ggatatccag 2281 acttagttgt aggagctttt ggtgtggatc gagctgtctt atacagagcc agacccgttg 2341 tcactgtaaa tgctggcctt gaagtgtacc ctagcatttt aaatcaagac aataagatct 2401 gcccgttgcc tgggacagct ctcaaggttt cctgttttaa tgtcagattc tgcttaaagg 2461 cagatggcaa gggaaccctt cctcggaagc tccacttcca ggtggagctt ctactggata 2521 aactcaagca gaagggagcc atccgacgag cactgtttct ccataacagg tccccggtcc 2581 actccaagac catgactgtt ttcagggggg gacagatgca gtgtgaggaa ctggtcgcct 2641 atcttcggga tgaatctgaa tttagagaca agctcactcc catcaccatt ttcatggagt 2701 atcggttgga ccagagaacg gctgctgatg ccacaggctt gcagcccatc ctgaaccagt 2761 tcactccggc caacgtcagt cggcaggctc atattctact tgactgtggt gaagacaatg 2821 tctgtaaacc taaattggaa gtttctgtaa atagtgatca aaagaagatc tatattgggg 2881 acgacaaccc tctgacactg actgtgaagg cgcagaatca aggggaaggc gcctatgaag 2941 ctgagctcat cgtttctatc ccaccgcagg ctgacttcat cggggttgtc cggaacaacg 3001 aagccttagc aagactgtcc tgtgcattta agacagaaaa ccaaactcgg caggtggtgt 3061 gcgaccttgg aaacccgatg aaggctggaa ctcaactgct cgctggtctt cgtttcagcg 3121 tgcaccagca gtcagagatg gatacttctg tgaagtttga cttgaaaatc cagagctcta 3181 attcttttga caatgtaagt ccagttgtgt cttacaaggt tgacctcgcc gttttggctg 3241 ctgtggagat aagaggagtt tcaagtccag atcatatctt tcttccaatt ccaaattggg 3301 agtacaagga aaaccctgag actgaagaag acgttgggcc tattgttcag cacatatatg 3361 agctgagaaa caatggtcca agttcattca gcaaggcaat tctaaatctc cagtggcctt 3421 acaaatacaa caacaacact ctgttgtaca ttcttcatta cgacattgac gggccaatga 3481 actgcacggc agatacagag atcaaccctt tgagaatcaa gacacccgaa aagaatgaca 3541 cagcggcggc gggacaggga gaaaggaatc atctcatcac caagcgggat ctcaccctca 3601 gagagggaga tgttcacact ttgggctgtg gaatcgccaa gtgcttgcag atcacctgcc 3661 aggttggtcg actggataga ggcaagagcg caatcctgta cgtgaaatcc ctgctgtgga 3721 ccgagacgtt catgaacaag gagaaccaga accattccta ttcgctgaag tcatctgctt 3781 cctttaatat catagaattc ccttacaaga acctgccgat cgaggatctc ttcaactcta 3841 cactagtcac cactaacatc acctggggca ttcagccggc gcccatgcct gtgcctgtgt 3901 gggtgatcat cttggcagtt ctcgcagggc tgctgctact ggctgtgttg gtatttgtaa 3961 tgtacaggat gggctttttc aaacgtgtcc gaccacctca agaagagcaa gaaagagaac 4021 agcttcagcc tcatgagaat ggcgaaggaa actctgaaac cgaattcatg GACTACAAAG 4081 ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGccgc 4141 gggagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccagccc 4201 gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc 4261 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 4321 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 4381 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc 4441 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 4501 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 4561 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 4621 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 4681 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 4741 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 4801 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 4861 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 4921 tggacgagct gtacaagtaa agcggccgct cgagtctaga gggcccgttt aaacccgctg 4981 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 5041 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 5101 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 5161 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 5221 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc 5281 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 5341 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 5401 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 5461 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 5521 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 5581 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 5641 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg 5701 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 5761 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 5821 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 5881 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 5941 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga 6001 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt 6061 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg 6121 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 6181 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 6241 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta 6301 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 6361 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 6421 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 6481 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 6541 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 6601 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc 6661 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 6721 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 6781 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 6841 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga 6901 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt 6961 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 7021 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg 7081 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 7141 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta 7201 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 7261 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 7321 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 7381 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 7441 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 7501 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 7561 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 7621 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 7681 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 7741 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 7801 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 7861 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 7921 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 7981 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 8041 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 8101 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 8161 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 8221 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 8281 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 8341 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 8401 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 8461 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 8521 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 8581 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 8641 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 8701 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 8761 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 8821 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 8881 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 8941 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 9001 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 9061 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 9121 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 9181 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 9241 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 9301 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 9361 gcacatttcc ccgaaaagtg ccacctgacg t

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.