pCMV-Itgav-3×FLAG-Neo plasmid

pCMV-Itgav-3×FLAG-Neo plasmid

PVT100203

pCMV-Itgav-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Itgav(mouse)-3×FLAG-Neo
Alias: NM_001398691.1;1110004F14Rik; 2610028E01Rik; CD51;D430040G12Rik
Gene lenght: 3129bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Itgav-3×FLAG-Neo plasmid Description

pCMV-Itgav-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Itgav-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatggc tgctcccggg cgcctgctgc 781 tacgccctcg ccccggcggc ctcctgctgc tgctgccggg tctcctgctg cccctcgccg 841 acgccttcaa cctggacgtc gaaagtcccg ccgagtatgc gggtcccgag ggaagttact 901 tcggattcgc cgtggacttc ttcgagccca gcacgtcctc gatgtttctc ctggtgggag 961 cccccaaagc gaacacgacc cagcctggga ttgtagaagg agggcaagtt ctcaaatgtg 1021 aatgctcatc cagccgccgg tgccagccca ttgagtttga ttcaacaggc aatcgagatt 1081 atgccaaaga tgacccactg gagtttaagt cccaccagtg gtttggagcc tctgtgaggt 1141 caaagcagga taaaatcttg gcctgtgctc cattgtacca ctggaggact gagatgaagc 1201 aggagagaga gccagttgga acctgctttc ttcaggacgg cacaaagacc gttgagtatg 1261 ctccatgcag gtcaaaaaat attgatgctg atggccaggg attttgtcaa ggaggattca 1321 gcattgattt tactaaagct gacagagtac ttctcggtgg tcctggtagc ttttattggc 1381 aaggtcagct catttcggac caagtggcag aaatcatatc taaatatgac ccaaatgtct 1441 acagcatcaa atataataac caattagcaa cacggactgc acaagcaatt tttgatgaca 1501 gttatttggg ttactctgtg gccgtgggag acttcaatgg tgacggcatt gaagattttg 1561 tctcaggagt tccaagagca gcaaggactt tgggaatggt ttatatttat gatgggaaaa 1621 atatgtcctc tttacacaat tttactggtg aacagatggc tgcgtatttt ggattttctg 1681 tagctgctac tgacattaat ggggatgatt acgcagatgt gtttattgga gcccccctgt 1741 tcatggaccg aggttccgat gggaaactcc aggaggttgg ccaggtctca gtgtctctgc 1801 agagagcagt gggagacttc cagactacaa agctgaacgg ctttgaggtt tttgccaggt 1861 ttggaagtgc catagctcca ttgggagacc tggaccagga tggcttcaat gatattgcaa 1921 ttgctgctcc ctatggtggt gaagataaga agggacttgt ttatatcttc aatggaaggt 1981 ccacaggctt gaattcggtg ccatctcaaa tcctcgaggg gcagtgggct gctcagagca 2041 tgcccccaag ctttggctat tcaatgaagg gagctacaga tgtagacagg aatggatatc 2101 cagacttagt tgtaggagct tttggtgtgg atcgagctgt cttatacaga gccagacccg 2161 ttgtcactgt aaatgctggc cttgaagtgt accctagcat tttaaatcaa gacaataaga 2221 tctgcccgtt gcctgggaca gctctcaagg tttcctgttt taatgtcaga ttctgcttaa 2281 aggcagatgg caagggaacc cttcctcgga agctccactt ccaggtggag cttctactgg 2341 ataaactcaa gcagaaggga gccatccgac gagcactgtt tctccataac aggtccccgg 2401 tccactccaa gaccatgact gttttcaggg ggggacagat gcagtgtgag gaactggtcg 2461 cctatcttcg ggatgaatct gaatttagag acaagctcac tcccatcacc attttcatgg 2521 agtatcggtt ggaccagaga acggctgctg atgccacagg cttgcagccc atcctgaacc 2581 agttcactcc ggccaacgtc agtcggcagg ctcatattct acttgactgt ggtgaagaca 2641 atgtctgtaa acctaaattg gaagtttctg taaatagtga tcaaaagaag atctatattg 2701 gggacgacaa ccctctgaca ctgactgtga aggcgcagaa tcaaggggaa ggcgcctatg 2761 aagctgagct catcgtttct atcccaccgc aggctgactt catcggggtt gtccggaaca 2821 acgaagcctt agcaagactg tcctgtgcat ttaagacaga aaaccaaact cggcaggtgg 2881 tgtgcgacct tggaaacccg atgaaggctg gaactcaact gctcgctggt cttcgtttca 2941 gcgtgcacca gcagtcagag atggatactt ctgtgaagtt tgacttgaaa atccagagct 3001 ctaattcttt tgacaatgta agtccagttg tgtcttacaa ggttgacctc gccgttttgg 3061 ctgctgtgga gataagagga gtttcaagtc cagatcatat ctttcttcca attccaaatt 3121 gggagtacaa ggaaaaccct gagactgaag aagacgttgg gcctattgtt cagcacatat 3181 atgagctgag aaacaatggt ccaagttcat tcagcaaggc aattctaaat ctccagtggc 3241 cttacaaata caacaacaac actctgttgt acattcttca ttacgacatt gacgggccaa 3301 tgaactgcac ggcagataca gagatcaacc ctttgagaat caagacaccc gaaaagaatg 3361 acacagcggc ggcgggacag ggagaaagga atcatctcat caccaagcgg gatctcaccc 3421 tcagagaggg agatgttcac actttgggct gtggaatcgc caagtgcttg cagatcacct 3481 gccaggttgg tcgactggat agaggcaaga gcgcaatcct gtacgtgaaa tccctgctgt 3541 ggaccgagac gttcatgaac aaggagaacc agaaccattc ctattcgctg aagtcatctg 3601 cttcctttaa tatcatagaa ttcccttaca agaacctgcc gatcgaggat ctcttcaact 3661 ctacactagt caccactaac atcacctggg gcattcagcc ggcgcccatg cctgtgcctg 3721 tgtgggtgat catcttggca gttctcgcag ggctgctgct actggctgtg ttggtatttg 3781 taatgtacag gatgggcttt ttcaaacgtg tccgaccacc tcaagaagag caagaaagag 3841 aacagcttca gcctcatgag aatggcgaag gaaactctga aaccGGATCC GACTACAAAG 3901 ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTAGT 3961 GAGGGCCCGA TATCTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG 4021 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA 4081 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT 4141 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT 4201 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC 4261 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG 4321 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC 4381 GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG 4441 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT 4501 GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC 4561 CTCCCCGCCT GGAAACGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA 4621 ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGGTGTTG GGTCGTTTGT 4681 TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA 4741 TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA 4801 AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCAG ATCTGCGCAG 4861 CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT 4921 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC 4981 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG 5041 CATCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 5101 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT 5161 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT 5221 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGGGGATT TCGGCCTATT GGTTAAAAAA 5281 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG 5341 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 5401 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 5461 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 5521 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 5581 GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 5641 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 5701 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 5761 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 5821 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 5881 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 5941 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 6001 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 6061 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 6121 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 6181 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 6241 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 6301 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 6361 GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 6421 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 6481 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGCGAAAT 6541 GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA 6601 TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG 6661 GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA 6721 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG 6781 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG 6841 CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC 6901 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT 6961 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 7021 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 7081 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 7141 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 7201 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 7261 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 7321 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 7381 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 7441 AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 7501 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 7561 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 7621 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 7681 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 7741 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 7801 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 7861 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 7921 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 7981 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 8041 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 8101 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 8161 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 8221 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 8281 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 8341 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 8401 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 8461 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 8521 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 8581 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 8641 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 8701 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 8761 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 8821 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 8881 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 8941 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 9001 AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG GTCGACTCTC 9061 AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATC TGCTCCCTGC TTGTGTGTTG 9121 GAGGTCGCTG AGTAGTGCGC GAGCAAAATT TAAGCTACAA CAAGGCAAGG CTTGACCGAC 9181 AATTGCATGA AGAATCTGCT TAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.