pCMV-Gmip-3×FLAG-Neo plasmid

pCMV-Gmip-3×FLAG-Neo plasmid

PVT110416

pCMV-Gmip-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Gmip(mouse)-3×FLAG-Neo
Alias: NM_198101.1;5031419I10Rik
Gene lenght: 2913
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Gmip-3×FLAG-Neo plasmid Description

pCMV-Gmip-3×FLAG-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Gmip-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatgga ctcagcggag acagagctaa 781 ccccagctcc tgagggcagg aagagataca gtgacatctt ccagagcctg gacaacctcg 841 agatatcatt ggggaacgta acttttgatc ccctggctgg agaccctgta cgcagagaag 901 acctggagcc tgacaaagct gacacagcca cagtggtgac tgaggagaac agtgaagcca 961 gtagttggcg ggatctctcc ccagaaggtc ctgcacccct cacagaggaa gaactggatt 1021 tgcgactcat tcggaccaag ggtggtgtag atgctgccct ggagtatgct aaagcatgga 1081 gccgctatgc caaggaactg ctggcctgga cagacaagag agccaactac gagctcgagt 1141 ttgctaagag tatcatgaag ctcgctgagg ctggcaaggt gtccattctc cagcagagcc 1201 aaatgccact ccagtacatc tacaccttgt ttctggagca tgacctcagc ctgggagccc 1261 tggccctgga gacagtggcc caacagaaga gagactacta ccagcctctg gcggccaaaa 1321 ggatggaaat tgagaagtgg aggaaggaat tcaaggaaca gtggctgaag gagcagaagc 1381 gcatgaatga ggcagtgcag gcactgcggc gctccgagct ccagtacatt caacgcagag 1441 aggacctgag ggcacgctcc caggggtccc ctgaggaccc tccttcccag gcatctcctg 1501 gatccaacaa gcaacaggag cgcagacggc gctcccgaga ggaggcacag gccaaggcgc 1561 atgaggcgga ggctctgtac caggcctgtg tccgggaggc caattctcgc cagcaggatc 1621 tggagaccac caagcggcgg atagtgtcac atgtgcgcaa gctggtgctg caaggagacg 1681 aagtgcttag gcgggtgacg ctgggtctgt ttgagctgcg aggggcgcag gcagagcgag 1741 gaccccgctc cttctctgct ctggctgagt gctgtgtgcc ctttgaacct ggccagcgct 1801 accaggagtt tgttcggaca ctgcagcctg gggccccacc gccgccatct ccggccttct 1861 gcttccagga gttcacagct gtggtgcaca gtttccctca ggacacaaaa aagaagtttt 1921 cggggcctct acctccaagg ctggaggagg agggttcccc tgagcctggc ccttgggagg 1981 ttgccagctt aggcagccag ggaatcccag gcagtgatgt ggacagtgta ggtggtggca 2041 gtgagtcccg gtctttggat tctcccactt ccagcccagg tgctggggcc cgccgacttg 2101 taaaggcttc gtccacaggc acggagtcct ccgatgactt tgaggagcga gaccctgatc 2161 tgggggatgg gatagagaat ggagtaggca gcccgttcag gaagtggaca ctgtccacag 2221 ctgctcaaac ccaccgactg cggcggctgc ggggcccagc caagtgcaga gaatgtgaag 2281 ccttcatggt cagcgggaca gaatgtgaag agtgcttttt gacctgtcac aagcgctgcc 2341 tggagaccct cctcatcctt tgtggacacc ggcggcttcc ggcccggatg tccctctttg 2401 gggttgactt cctacagctc cccagagatt tccctgagga ggttcccttt gtgattacca 2461 gatgcacagc tgagatagag caccgtgccc tgggcttgca gggtatctat cgggtcagcg 2521 ggtctcgggt acgtgtggag cggctgtgcc aggcctttga gaatggccga gcactggtcg 2581 agctgtccgg gaactctcct cacgatatca ccagcgttct caagcgattt cttcaggagc 2641 tcactgatcc cgtggtcccc ttccacttgt acgacgcctt catctctctg gcaaagaccc 2701 tgcatgcaga ccctggggac gaccctggga cccccaaccc cagccctgag attattcgct 2761 cgctgaagac cctcttggtg cagctgcctg actctaacta cagtaccctg cgacacctgg 2821 tggcccatct gttcagggtg gctgctcggt ttgaagaaaa caagatgtct gccaacaatc 2881 tgggaattgt atttgggcct acactgctgc ggccaccaga tggacccagg gccaccggtg 2941 ccagccctgt ggcctgtctg ctggactccg gtcaccaggc tcagctggtt gagttcctca 3001 ttgtgcacta tgagcagatc tttggaatgg atgagctccc tctggcctcg gagcctctga 3061 cccaagaccc tggcttggct cctgcatgcc ttgaatccag tccccagcac ccagcctcac 3121 ttcttgccca agacacacag cccctgacca tagccttgga ttccagccca gaccccaaac 3181 accacagtgc cttggagaag tgccccgagg tcacacctcc tgagcttgca actctgcaga 3241 gggaccagag ggaggaggag gtggaagaca ccagagatgg ggcaggggat gggtccagcc 3301 actgccctga ggacttggcc ctgggagcac aatcccgggg ccacttcagc cgccagccag 3361 tgaagtattc ccggggaggt gtgaggccag ttactcatca actctccagc ctggctctgg 3421 tggcctccaa gctgtgtgag gagactcctg tcactgtctc agcagtgcac cgaggtagtt 3481 tgagggtacg aggtttgggc cctgctgctg cctgccctga aggcagcccc ctgcgccgga 3541 accctctgcc caagcacttt gagatcaccc aggagacagc ccgacttctc tccaagctga 3601 acagtgatgc tgtgtccagg accacctgtt gtgctgaccc agagcctgag gagtctgaag 3661 agcacctcGG ATCCGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT 3721 ACAAGGATGA CGATGACAAG TAGTGAGGGC CCGATATCTC GACAATCAAC CTCTGGATTA 3781 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG 3841 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC 3901 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA 3961 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC 4021 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT 4081 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC 4141 CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG 4201 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC 4261 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC 4321 GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCCTGGAAAC GGGGGAGGCT AACTGAAACA 4381 CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA 4441 ACGCACGGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC 4501 TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC 4561 ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG 4621 CCCTGCCATA GCAGATCTGC GCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG 4681 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG 4741 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT 4801 TCCCCGTCAA GCTCTAAATC GGGGCATCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA 4861 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA 4921 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA 4981 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGGG 5041 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT 5101 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 5161 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 5221 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 5281 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 5341 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG 5401 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 5461 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 5521 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 5581 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 5641 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 5701 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 5761 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 5821 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 5881 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 5941 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 6001 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 6061 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 6121 TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC 6181 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 6241 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 6301 GCGGGACTCT GGGGTTCGCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA 6361 TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC 6421 CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT 6481 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA 6541 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA 6601 TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC 6661 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG 6721 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC 6781 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG 6841 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC 6901 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 6961 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 7021 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 7081 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 7141 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 7201 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA 7261 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 7321 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 7381 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 7441 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG 7501 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 7561 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 7621 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 7681 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 7741 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 7801 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 7861 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 7921 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 7981 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 8041 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 8101 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 8161 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 8221 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 8281 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 8341 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 8401 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 8461 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 8521 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 8581 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 8641 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 8701 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 8761 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT 8821 CCCGATCCCC TATGGTCGAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG 8881 TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA AATTTAAGCT 8941 ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.