pCMV-3×HA-Dis3-Puro plasmid

pCMV-3×HA-Dis3-Puro plasmid

PVT106669

pCMV-3×HA-Dis3-Puro plasmid Information

Plasmid name: pCMV-3×HA-Dis3(mouse)-Puro
Alias: NM_028315.2;2810028N01Rik
Gene lenght: 2877
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×HA-Dis3-Puro plasmid Description

pCMV-3×HA-Dis3-Puro is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×HA-Dis3-Puro plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 AAGCTTTCTA GAGAATTCgc caccatgTAC CCATACGACG TACCTGATTA TGCAACTTAT 961 CCTTATGATG TACCTGATTA TGCTAGtTAT CCTTATGATG TCCCAGACTA CGCAGGATCC 1021 atgctcaggt ccaagacgtt cttgaagaag acccgcgcgg gcggcgtggt gaagatcgtg 1081 cgcgagcact acctgcggga tgacatcggc tgcggcgcgc cggcttgctc ggcctgcggc 1141 ggggcgcacg cgggcccggc cctggagctg cagccccggg accaggcgag cagcctctgc 1201 ccgtggccgc actaccttct gccggacacc aatgtgctgc tgcaccagat tgatgtcctc 1261 gaacacccgg ccatcagaaa tgtcattgtg ctacaaacag tgatgcaaga agtgagaaac 1321 cggagcgccc ccatctacaa gcgaatcagg gatgtgacca ataaccagga aaagcatttc 1381 tataccttca ctaatgagca ccataaagaa acctacatcg agcaagagca gggagagaat 1441 gccaatgaca ggaatgacag agccatccga gtcgcagcga agtggtacaa cgagcacctg 1501 aagagggtgg cagcagacag tcagctgcaa gttatcctga taaccaatga caggaagaac 1561 aaagagaaag ctgtgcaaga ggggatacca gccttcacgt gtgaagaata cgtaaagagc 1621 ctgactgcta accctgaact tatagaccgt cttgcttact tgtccgatga aatgaatgaa 1681 atagaaagtg ggaaaataat attttcagag catcttccct taagcaagct ccaacaaggc 1741 ataaaatctg gttcctatct tcaaggaaca ttcagagcta gcagggaaaa ttatttggag 1801 gctacagtat ggattcatgg agacaaagaa gaggaaaaag agatacttat acagggaatt 1861 aagcatctaa acagagctgt gcatgaagac attgtggccg tggagctact gcccaggagc 1921 cagtgggtgg caccgtcttc cgtggtttta gacgatgaag gtcaaaatga agacgatgtg 1981 gagaaagatg aggagagaga actcctgctt aagactgctg taagtgaaaa aatgttacgg 2041 cctacaggtc gagttgtggg gataataaaa aggaactgga gaccgtattg tggcatgctt 2101 tccaagtctg atattaagga gtcaagaaga catctcttta cacccgctga taagagaatt 2161 ccacgaattc ggatagaaac cagacaggct tctgcgttag aaggacggag aattattgtc 2221 gctattgatg gttggcctag aaattctaga tatccaaatg gacactttgt aaagaattta 2281 ggcgatgttg gagagaagga gacagaaacg gaagtgttgc tgctcgagca cgatgttcct 2341 catcagccct tttcccaggc tgtgcttagc ttcctgccca ggatgccctg gagcattact 2401 gaggaggaca tgaaaaaccg agaagacctg agacatctgt gtgtttgcag tgtggaccct 2461 ccagggtgca ctgacataga tgacgctctg cattgtagag agctcagcaa tggaaacttg 2521 gaggttggtg ttcatattgc ggatgttagc cattttatca ggccaggaaa tgcgttggat 2581 caagaatctg caagaagagg aacaactgtt tatctttgtg aaaagaggat tgacatggtt 2641 ccagagttgc tcagctccaa cctctgttcc ttaagatcca acgttgacag gttggcattt 2701 tcctgtattt gggaaatgaa tcataatgct gaaatattaa aaacgagatt taccaaaagt 2761 gtcattaatt caaaggcttc tcttacgtac gcggaagcac agatgagaat tgattcggcg 2821 gctatgaatg atgatattac cactagtctc cgtggactca atcagctggc taaaattcta 2881 aaaaagggaa ggattgaaaa gggggctttg actctgtctt ctccagagat ccgattccac 2941 atggacagtg aaacccacga cccaatcgac ctgcagacga aagagctgag agaaacaaat 3001 tccatggtgg aagaatttat gttacttgct aatatttctg tcgcaaaaaa aattcatgaa 3061 gagttttctg aacatgctct gcttcggaaa cacccagctc cgcctccctc gaattatgac 3121 attcttgtta aggctgcaaa gtccaagaat ttgcaaatta agactgatac agccaaatct 3181 ttggccgact ctttggaccg ggctgaatct ccggatttcc catacctgaa tactctatta 3241 aggatactgg ccactcgctg tatgatgcaa gctgtgtact tctgctctgg gatggataat 3301 gattttcatc actatggctt agcctccccc atatacacac atttcacctc tcctatcaga 3361 agatatgcag acataattgt ccatcggcta ttagctgtgg cgattggggc tgactgtact 3421 taccctgagt tgacagacaa acacaagctt tcagatatat gtaaaaacct caatttccgg 3481 cataaaatgg ctcagtatgc ccagcgtgct tcggtggctt ttcatacaca gttgtttttc 3541 aaaagcaaag gaatagtaag tgaagaagcc tatattctct ttgtaaggaa aaatgcaatt 3601 gtggtgttaa ttccaaagta tggcttagaa ggtacagttt tctttgaaga aaaagataaa 3661 ccaaagccac gccttgctta cgatgatgag atcccttccc tgagaataga aggtacagtg 3721 ttccatgtgt ttgataaggt taaagtgaag atcacattag attcatcaaa tcttcaacat 3781 cagaaaatcc gcatggccct tgtagaacca cagatcccag gaataaatat tcctcctaat 3841 gttgcagaca aggctcttac tgcaccaggg ggaaagaaga ggaagcttga gaagtagCTC 3901 GAGTGACCGG TaAGTTTAAA CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA 3961 TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC 4021 CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG 4081 GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT 4141 GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG 4201 TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 4261 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 4321 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 4381 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 4441 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 4501 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 4561 GATTTATAAG GGATTTTGGC CATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 4621 AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG 4681 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG 4741 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG 4801 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC 4861 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG 4921 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 4981 AGCTCCCggg atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 5041 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 5101 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 5161 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 5221 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 5281 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 5341 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 5401 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 5461 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 5521 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 5581 cggtgcctga TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA 5641 TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG 5701 GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT 5761 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT 5821 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG 5881 TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG 5941 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 6001 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 6061 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 6121 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 6181 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 6241 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 6301 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 6361 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 6421 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 6481 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 6541 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 6601 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 6661 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 6721 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 6781 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 6841 AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 6901 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 6961 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 7021 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 7081 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 7141 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 7201 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 7261 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 7321 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 7381 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG 7441 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT 7501 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT 7561 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT 7621 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 7681 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT 7741 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG 7801 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 7861 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG 7921 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 7981 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 8041 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.