pLV3-EF1a-SLCAR-V5-FLAG-Myc-CD

pLV3-EF1a-SLCAR-V5-FLAG-Myc-CD19-28z plasmid

PVT110412

pLV3-EF1a-SLCAR-V5-FLAG-Myc-CD19-28z plasmid Information

Plasmid name: pLV3-EF1a-SLCAR-V5-FLAG-Myc-CD19-28z
Alias:
Gene lenght: 1465
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF-1α-F1
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-SLCAR-V5-FLAG-Myc-CD19-28z plasmid Description

pLV3-EF1a-SLCAR-V5-FLAG-Myc-CD19-28z It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-EF1a-SLCAR-V5-FLAG-Myc-CD19-28z plasmid Sequence

1 catgtgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg 61 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga 121 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa 181 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg 241 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag 301 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 361 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 421 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 481 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 541 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 601 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 661 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 721 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 781 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg 841 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 901 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 961 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 1021 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 1081 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 1141 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 1201 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 1261 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 1321 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 1381 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt 1441 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca 1501 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag 1561 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat 1621 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg 1681 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt 1741 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca 1801 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca 1861 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg 1921 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt 1981 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag 2041 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag 2101 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta 2161 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag 2221 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac 2281 cattatcgtt tcagacccac ctcccaaccc cgaggggacc ccgggtttat tacagggaca 2341 gcagagatcc actttggcgc cggctcgagt tttaaaagaa aaggggggat tggggggtac 2401 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa 2461 aaacaaatta caaaaattca aaatttttcg agtggctccg gtgcccgtca gtgggcagag 2521 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc 2581 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 2641 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 2701 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc 2761 tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc tgcagtacgt 2821 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta 2881 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt 2941 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta 3001 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg 3061 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc 3121 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg 3181 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc 3241 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc 3301 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc 3361 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga 3421 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac 3481 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt 3541 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt 3601 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc 3661 atttcaggtg tcgtgagGGA ATTCgtgtcg tgacgcggga tccgccacca tgctcaggct 3721 gctcttggct ctcaacttat tcccttcaat tcaagtaaca ggagggtctt cgGGTAAGCC 3781 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGGGTTCA GGAGACTACA AGGACGACGA 3841 TGACAAGGGC GGTTCTGAGC AGAAGCTGAT CTCAGAGGAG GACCTGgaca tccagatgac 3901 acagactaca tcctccctgt ctgcctctct gggagacaga gtcaccatca gttgcagggc 3961 aagtcaggac atctctaagt atttgaattg gtatcagcag aaaccagatg gaactgttaa 4021 actcctgatc taccatacat caagattaca ctcaggagtc ccatcaaggt tcagtggcag 4081 tgggtctgga acagattatt ctctcaccat tagcaacctg gagcaagaag atattgccac 4141 ttacttttgc caacagggta atacgcttcc gtacacgttc ggagggggga ctaagttgga 4201 aataacaggc tccacctctg gatccggcaa gcccggatct ggcgagggat ccaccaaggg 4261 cgaggtgaaa ctgcaggagt caggacctgg cctggtggcg ccctcacaga gcctgtccgt 4321 cacatgcact gtctcagggg tctcattacc cgactatggt gtaagctgga ttcgccagcc 4381 tccacgaaag ggtctggagt ggctgggagt aatatggggt agtgaaacca catactataa 4441 ttcagctctc aaatccagac tgaccatcat caaggacaac tccaagagcc aagttttctt 4501 aaaaatgaac agtctgcaaa ctgatgacac agccatttac tactgtgcca aacattatta 4561 ctacggtggt agctatgcta tggactactg gggtcaagga acctcagtca ccgtctcctc 4621 agcggccgca ggtaccacca caacgcccgc tcctcggcca ccgacgccag cgccaactat 4681 tgcgagtcag cctctcagtc tgcgacctga ggcttgtcga ccagcagccg gaggcgcagt 4741 gcacacgagg gggctggact tcgcctgtga tagaagacct ccttctaagc ccttttgggt 4801 gctggtggtg gttggtggag tcctggcttg ctatagcttg ctagtaacag tggcctttat 4861 tattttctgg gtgaggagta agaggagcag gctcctgcac agtgactaca tgaacatgac 4921 tcccaggcgg cccggaccca cccgcaagca ttaccagccc tatgccccac cacgcgactt 4981 cgcagcctat cgctccgcta gcctgagagt gaagttcagc aggagcgcag acgcccccgc 5041 gtaccagcag ggccagaacc agctctataa cgagctcaat ctaggacgaa gagaggagta 5101 cgatgttttg gacaagagac gtggccggga ccctgagatg gggggaaagc cgcagagaag 5161 gaagaaccct caggaaggcc tgtacaatga actgcagaaa gataagatgg cggaggccta 5221 cagtgagatt gggatgaaag gcgagcgccg gaggggcaag gggcacgatg gcctttacca 5281 gggtctcagt acagccacca aggacaccta cgacgccctt cacatgcagg ccctgccccc 5341 tcgcgctagc gccacgaact tctctctgtt aaagcaagca ggcgacgtgg aagaaaaccc 5401 cggtcccgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 5461 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 5521 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 5581 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat 5641 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 5701 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 5761 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 5821 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa 5881 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 5941 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 6001 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 6061 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 6121 gtaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 6181 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 6241 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 6301 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 6361 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 6421 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 6481 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg 6541 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 6601 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 6661 cggcctcttc cgcgtctttg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 6721 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 6781 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt 6841 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 6901 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 6961 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 7021 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg 7081 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 7141 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 7201 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 7261 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 7321 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 7381 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg 7441 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 7501 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 7561 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc 7621 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 7681 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 7741 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 7801 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 7861 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 7921 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 7981 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 8041 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa 8101 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 8161 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 8221 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 8281 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 8341 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 8401 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 8461 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 8521 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 8581 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 8641 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 8701 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 8761 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 8821 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 8881 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 8941 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 9001 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 9061 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 9121 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 9181 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 9241 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 9301 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 9361 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 9421 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 9481 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 9541 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 9601 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 9661 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 9721 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 9781 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 9841 tacggttcct ggccttttgc tggccttttg ctca

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.