pLV3-CMV-LCN2-3×HA-CopGFP-Puro

pLV3-CMV-LCN2-3×HA-CopGFP-Puro plasmid

PVT110321

pLV3-CMV-LCN2-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-LCN2(human)-3×HA-CopGFP-Puro
Alias: NM_005564.5;24p3; MSFI; NGAL; p25
Gene lenght: 594
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-LCN2-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-LCN2-3×HA-CopGFP-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-LCN2-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc ccctaggtct cctgtggctg 2341 ggcctagccc tgttgggggc tctgcatgcc caggcccagg actccacctc agacctgatc 2401 ccagccccac ctctgagcaa ggtccctctg cagcagaact tccaggacaa ccaattccag 2461 gggaagtggt atgtggtagg cctggcaggg aatgcaattc tcagagaaga caaagacccg 2521 caaaagatgt atgccaccat ctatgagctg aaagaagaca agagctacaa tgtcacctcc 2581 gtcctgttta ggaaaaagaa gtgtgactac tggatcagga cttttgttcc aggttgccag 2641 cccggcgagt tcacgctggg caacattaag agttaccctg gattaacgag ttacctcgtc 2701 cgagtggtga gcaccaacta caaccagcat gctatggtgt tcttcaagaa agtttctcaa 2761 aacagggagt acttcaagat caccctctac gggagaacca aggagctgac ttcggaacta 2821 aaggagaact tcatccgctt ctccaaatct ctgggcctcc ctgaaaacca catcgtcttc 2881 cctgtcccaa tcgaccagtg tatcgacggc CTCGAGTACC CATACGACGT ACCTGATTAT 2941 GCAACTTATC CTTATGATGT ACCTGATTAT GCTAGCTATC CTTATGATGT CCCAGACTAC 3001 GCATAGggat ccCTGCGATA TCCAGCgcgg ccgcGaagga tctgcgatcg ctccggtgcc 3061 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 3121 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 3181 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 3241 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 3301 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 3361 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 3421 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 3481 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 3541 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag 3601 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac 3661 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc 3721 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg 3781 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg 3841 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc 3901 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag 3961 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc 4021 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc 4081 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac 4141 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc 4201 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc 4261 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc 4321 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt 4381 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc 4441 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 4501 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 4561 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 4621 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 4681 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 4741 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 4801 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 4861 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 4921 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 4981 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg 5041 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 5101 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 5161 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 5221 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 5281 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 5341 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 5401 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 5461 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 5521 gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg 5581 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 5641 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat 5701 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 5761 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 5821 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 5881 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 5941 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 6001 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 6061 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 6121 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 6181 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 6241 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg 6301 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 6361 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 6421 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 6481 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 6541 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 6601 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 6661 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 6721 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 6781 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 6841 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 6901 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 6961 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 7021 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 7081 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 7141 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 7201 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 7261 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 7321 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 7381 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 7441 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 7501 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 7561 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 7621 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 7681 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 7741 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 7801 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 7861 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 7921 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 7981 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 8041 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 8101 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 8161 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 8221 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 8281 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 8341 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 8401 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 8461 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 8521 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 8581 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 8641 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 8701 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 8761 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 8821 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 8881 gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.