pLenti_EF1a_EV plasmid

pLenti_EF1a_EV plasmid

PVT110072

pLenti_EF1a_EV plasmid Information

Plasmid name: pLenti_EF1a_EV
Alias:
Gene lenght: 136
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species:
Prokaryotic resistance: Kan
Screening marker: Hyg,Neo/G418
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stable
Growth Temperature: 30°C
Vector backbone:
Forward primer: EF-1α-F1
Reverse primer:
Copy number: High
Induction:

pLenti_EF1a_EV plasmid Description

pLenti_EF1a_EV It is a plasmid suitable for gene expression in mammalian cells.

pLenti_EF1a_EV plasmid Sequence

1 gatctggcca atcacaagtt tgtacaaaaa agcaggcttt aaaggaacca attcagtcga 61 ctggatccgg taccgaattc gcggccgcac tcgagatatc tagacccagc tttcttgtac 121 aaagtggtgg gaggaggtgg atctggcgac tacaaggacg acgatgacaa gtgactcgag 181 gttaacgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct 241 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc 301 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct 361 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga 421 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg 481 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct 541 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg 601 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc 661 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatcccgcc accatgaaaa 721 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct 781 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag 841 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg 901 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat 961 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc 1021 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg 1081 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc 1141 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc 1201 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc 1261 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca 1321 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg 1381 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg 1441 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc 1501 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt 1561 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga 1621 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag 1681 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagt 1741 gtacaagtag gaattcgtcg agggacctaa taacttcgta tagcatacat tatacgaagt 1801 tatacatgtt taagggttcc ggttccacta ggtacaattc gatatcaagc ttatcgataa 1861 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 1921 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 1981 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 2041 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 2101 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 2161 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 2221 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 2281 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 2341 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 2401 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg 2461 acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca 2521 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac 2581 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 2641 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg 2701 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 2761 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 2821 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 2881 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 2941 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca 3001 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 3061 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc 3121 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 3181 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 3241 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattagcat 3301 gtgagcaaaa ggccagcata aatcaaaaga atagaccgag atagggttga gtgttgttcc 3361 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 3421 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 3481 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 3541 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 3601 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 3661 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 3721 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 3781 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg 3841 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 3901 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 3961 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 4021 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 4081 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 4141 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg 4201 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 4261 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 4321 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 4381 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 4441 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 4501 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 4561 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 4621 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 4681 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga 4741 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 4801 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 4861 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 4921 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 4981 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 5041 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 5101 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 5161 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc 5221 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa 5281 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc 5341 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 5401 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata 5461 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 5521 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 5581 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 5641 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 5701 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 5761 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 5821 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 5881 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 5941 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 6001 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 6061 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 6121 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 6181 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 6241 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 6301 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 6361 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 6421 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 6481 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 6541 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 6601 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 6661 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 6721 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 6781 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 6841 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt 6901 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 6961 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 7021 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 7081 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc 7141 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac 7201 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat 7261 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa 7321 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga 7381 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc 7441 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat 7501 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 7561 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg 7621 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 7681 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 7741 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 7801 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 7861 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 7921 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 7981 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 8041 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 8101 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 8161 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 8221 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 8281 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 8341 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 8401 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg 8461 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg 8521 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga 8581 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga 8641 tccagtttgg ttagtaccgg gcccgctcta gccctagatt taaatggctc cggtgcccgt 8701 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 8761 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 8821 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 8881 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc 8941 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg 9001 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct 9061 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc 9121 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta 9181 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta 9241 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg 9301 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga 9361 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg 9421 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa 9481 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga 9541 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct 9601 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt 9661 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact 9721 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt 9781 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt 9841 tttcttccat ttcaggtgtc gtgaggaatt agcttggtac taatacgact cactataggg 9901 agacccaagc tggctagccg cggaatctag actgccggat cgaattgtcg acgaattcg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.