pCMV-TIA1-EYFP-Neo plasmid

pCMV-TIA1-EYFP-Neo plasmid

PVT109765

pCMV-TIA1-EYFP-Neo plasmid Information

Plasmid name: pCMV-TIA1(human)-EYFP-Neo
Alias: NM_022173.4;ALS26; TIA-1; WDM
Gene lenght: 1158
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Yellow
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-TIA1-EYFP-Neo plasmid Description

pCMV-TIA1-EYFP-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-TIA1-EYFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcGCCACC atggaggacg agatgcccaa 661 gactctatac gtcggtaacc tttccagaga tgtgacagaa gctctaattc tgcaactctt 721 tagccagatt ggaccttgta aaaactgcaa aatgattatg gatacagctg gaaatgatcc 781 ctattgtttt gtggagtttc atgagcatcg tcatgcagct gcagcattag ctgctatgaa 841 tggacggaag ataatgggta aggaagtcaa agtgaattgg gcaacaaccc ctagcagtca 901 aaagaaagat acaagcagta gtaccgttgt cagcacacag cgttcacaag atcatttcca 961 tgtctttgtt ggtgatctca gcccagaaat tacaactgaa gatataaaag ctgcttttgc 1021 accatttgga agaatatcag atgcccgagt ggtaaaagac atggcaacag gaaagtctaa 1081 gggatatggc tttgtctcct ttttcaacaa atgggatgct gaaaacgcca ttcaacagat 1141 gggtggccag tggcttggtg gaagacaaat cagaactaac tgggcaaccc gaaagcctcc 1201 cgctccaaag agtacatatg agtcaaatac caaacagcta tcatatgatg aggttgtaaa 1261 tcagtctagt ccaagcaact gtactgtata ctgtggaggt gttacttctg ggctaacaga 1321 acaactaatg cgtcagactt tttcaccatt tggacaaata atggaaattc gagtctttcc 1381 agataaagga tattcatttg ttcggttcaa ttcccatgaa agtgcagcac atgcaattgt 1441 ttctgttaat ggtactacca ttgaaggtca tgttgtgaaa tgctattggg gcaaagaaac 1501 tcttgatatg ataaatcccg tgcaacagca gaatcaaatt ggatatcccc aaccttatgg 1561 ccagtggggc cagtggtatg gaaatgcaca acaaattggc cagtatatgc ctaatggttg 1621 gcaagttcct gcatatggaa tgtatggcca ggcatggaac cagcaaggat ttaatcagac 1681 acagtcttct gcaccatgga tgggaccaaa ttatggagtg caaccgcctc aagggcaaaa 1741 tggcagcatg ttgcccaatc agccttctgg gtatcgagtg gcagggtatg aaacccagGA 1801 ATTCggtgga ggcggatcag gaggcggtgg atctCCggat ccaccggtcg ccaccatggt 1861 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 1921 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 1981 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 2041 gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca 2101 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 2161 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 2221 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 2281 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 2341 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 2401 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 2461 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 2521 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg 2581 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 2641 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 2701 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 2761 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa 2821 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 2881 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 2941 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 3001 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 3061 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 3121 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 3181 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 3241 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg 3301 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 3361 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc 3421 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 3481 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 3541 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 3601 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 3661 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 3721 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca 3781 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 3841 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 3901 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 3961 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac 4021 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 4081 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 4141 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 4201 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 4261 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 4321 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 4381 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 4441 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 4501 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 4561 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa 4621 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 4681 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 4741 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag 4801 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac 4861 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat 4921 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac 4981 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc 5041 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 5101 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 5161 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 5221 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 5281 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 5341 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 5401 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 5461 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 5521 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 5581 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 5641 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 5701 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 5761 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 5821 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 5881 tctgtggata accgtattac cgccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.