pLV3-CMV-TFRC-3×Myc-Puro plasm

pLV3-CMV-TFRC-3×Myc-Puro plasmid

PVT97190

pLV3-CMV-TFRC-3×Myc-Puro plasmid Information

Plasmid name: pLV3-CMV-TFRC(human)-3×Myc-Puro
Alias: NM_003234.4;CD71; IMD46; p90; T9; TFR; TFR1; TR; TRFR
Gene lenght: 2280bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TFRC-3×Myc-Puro plasmid Description

pLV3-CMV-TFRC-3×Myc-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TFRC-3×Myc-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgatggat caagctagat cagcattctc taacttgttt 2341 ggtggagaac cattgtcata tacccggttc agcctggctc ggcaagtaga tggcgataac 2401 agtcatgtgg agatgaaact tgctgtagat gaagaagaaa atgctgacaa taacacaaag 2461 gccaatgtca caaaaccaaa aaggtgtagt ggaagtatct gctatgggac tattgctgtg 2521 atcgtctttt tcttgattgg atttatgatt ggctacttgg gctattgtaa aggggtagaa 2581 ccaaaaactg agtgtgagag actggcagga accgagtctc cagtgaggga ggagccagga 2641 gaggacttcc ctgcagcacg tcgcttatat tgggatgacc tgaagagaaa gttgtcggag 2701 aaactggaca gcacagactt caccggcacc atcaagctgc tgaatgaaaa ttcatatgtc 2761 cctcgtgagg ctggatctca aaaagatgaa aatcttgcgt tgtatgttga aaatcaattt 2821 cgtgaattta aactcagcaa agtctggcgt gatcaacatt ttgttaagat tcaggtcaaa 2881 gacagcgctc aaaactcggt gatcatagtt gataagaacg gtagacttgt ttacctggtg 2941 gagaatcctg ggggttatgt ggcgtatagt aaggctgcaa cagttactgg taaactggtc 3001 catgctaatt ttggtactaa aaaagatttt gaggatttat acactcctgt gaatggatct 3061 atagtgattg tcagagcagg gaaaatcacc tttgcagaaa aggttgcaaa tgctgaaagc 3121 ttaaatgcaa ttggtgtgtt gatatacatg gaccagacta aatttcccat tgttaacgca 3181 gaactttcat tctttggaca tgctcatctg gggacaggtg acccttacac acctggattc 3241 ccttccttca atcacactca gtttccacca tctcggtcat caggattgcc taatatacct 3301 gtccagacaa tctccagagc tgctgcagaa aagctgtttg ggaatatgga aggagactgt 3361 ccctctgact ggaaaacaga ctctacatgt aggatggtaa cctcagaaag caagaatgtg 3421 aagctcactg tgagcaatgt gctgaaagag ataaaaattc ttaacatctt tggagttatt 3481 aaaggctttg tagaaccaga tcactatgtt gtagttgggg cccagagaga tgcatggggc 3541 cctggagctg caaaatccgg tgtaggcaca gctctcctat tgaaacttgc ccagatgttc 3601 tcagatatgg tcttaaaaga tgggtttcag cccagcagaa gcattatctt tgccagttgg 3661 agtgctggag actttggatc ggttggtgcc actgaatggc tagagggata cctttcgtcc 3721 ctgcatttaa aggctttcac ttatattaat ctggataaag cggttcttgg taccagcaac 3781 ttcaaggttt ctgccagccc actgttgtat acgcttattg agaaaacaat gcaaaatgtg 3841 aagcatccgg ttactgggca atttctatat caggacagca actgggccag caaagttgag 3901 aaactcactt tagacaatgc tgctttccct ttccttgcat attctggaat cccagcagtt 3961 tctttctgtt tttgcgagga cacagattat ccttatttgg gtaccaccat ggacacctat 4021 aaggaactga ttgagaggat tcctgagttg aacaaagtgg cacgagcagc tgcagaggtc 4081 gctggtcagt tcgtgattaa actaacccat gatgttgaat tgaacctgga ctatgagagg 4141 tacaacagcc aactgctttc atttgtgagg gatctgaacc aatacagagc agacataaag 4201 gaaatgggcc tgagtttaca gtggctgtat tctgctcgtg gagacttctt ccgtgctact 4261 tccagactaa caacagattt cgggaatgct gagaaaacag acagatttgt catgaagaaa 4321 ctcaatgatc gtgtcatgag agtggagtat cacttcctct ctccctacgt atctccaaaa 4381 gagtctcctt tccgacatgt cttctggggc tccggctctc acacgctgcc agctttactg 4441 gagaacttga aactgcgtaa acaaaataac ggtgctttta atgaaacgct gttcagaaac 4501 cagttggctc tagctacttg gactattcag ggagctgcaa atgccctctc tggtgacgtt 4561 tgggacattg acaatgagtt tggatccGAA CAAAAACTAA TATCAGAGGA AGATTTGGAA 4621 CAGAAACTAA TCAGTGAAGA AGATTTAGAG CAAAAGTTAA TTTCCGAGGA GGACTTATAG 4681 CGGCCGCGAA GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC 4741 ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG 4801 CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG 4861 GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC 4921 GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT 4981 ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC 5041 TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT 5101 CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG 5161 CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG 5221 TGACCGGCGC CTACGCTAGA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA 5281 CGACGTCCCC CGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG 5341 CCACACCGTC GACCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT 5401 CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC 5461 GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG 5521 CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC 5581 GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA 5641 CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC 5701 CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT 5761 CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC 5821 CCGCAAGCCC GGTGCCTGAG TCGACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT 5881 GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC 5941 TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG 6001 GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC 6061 TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC 6121 CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC 6181 CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA 6241 ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC 6301 CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC 6361 GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG 6421 GGCCGCCTCC CCGCCTGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA 6481 GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAAATAAG 6541 ATCTGCTTTT TGCTTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT 6601 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG 6661 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT 6721 CAGTGTGGAA AATCTCTAGC AGTAGTAGTT CATGTCATCT TATTATTCAG TATTTATAAC 6781 TTGCAAAGAA ATGAATATCA GAGAGTGAGA GGAACTTGTT TATTGCAGCT TATAATGGTT 6841 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 6901 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGCTCTAG CTATCCCGCC 6961 CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA 7021 TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT 7081 TGGAGGCCTA GACTTTTGCA GAGACGGCCC AAATTCGTAA TCATGGTCAT AGCTGTTTCC 7141 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG 7201 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC 7261 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG 7321 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC 7381 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 7441 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 7501 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 7561 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 7621 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 7681 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 7741 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 7801 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 7861 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 7921 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG 7981 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 8041 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 8101 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 8161 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 8221 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 8281 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 8341 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 8401 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 8461 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 8521 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 8581 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 8641 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 8701 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 8761 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 8821 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 8881 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 8941 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 9001 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 9061 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 9121 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 9181 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 9241 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT 9301 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG 9361 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA 9421 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG 9481 GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG 9541 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG 9601 GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC 9661 GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG 9721 ACGTTGTAAA ACGACGGCCA GTGCCAAGCT G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.