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- LSMBIO
- 国产
- PVT109389
- 2026年03月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pLV3-U6-Slc10a2-sgRNA1-Cas9-EGFP-Puro plasmid
- 库存：
20
- 供应商：
LSMBIO
- 规格：
1ug
pLV3-U6-Slc10a2-sgRNA1-Cas9-EGFP-Puro plasmid
PVT109389
pLV3-U6-Slc10a2-sgRNA1-Cas9-EGFP-Puro plasmid Information
Plasmid name: pLV3-U6-Slc10a2(mouse)-sgRNA1-Cas9-EGFP-Puro
Alias: CAAAGAGGCATCATTCCAA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene knockout
Fragment type: CRISPR
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:
pLV3-U6-Slc10a2-sgRNA1-Cas9-EGFP-Puro plasmid Description
pLV3-U6-Slc10a2-sgRNA1-Cas9-EGFP-Puro plasmid Sequence
//
Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.
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文献和实验and homologous recombination. These tools provide researchers with new instruments that accelerateboth forward and reverse genetics efforts. Key words Genome engineering , Cas9 , CRISPR , CRISPR-Cas9 , PAM , Guide RNA , sgRNA , DNA cleavage , Mutagenesis
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
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