pLV3-CMV-turboRFP-CSF1-EF1a-Hy

pLV3-CMV-turboRFP-CSF1-EF1a-Hyg plasmid

PVT108788

pLV3-CMV-turboRFP-CSF1-EF1a-Hyg plasmid Information

Plasmid name: pLV3-CMV-turboRFP-CSF1(human)-EF1a-Hyg
Alias: NM_000757.6;CSF-1; MCSF
Gene lenght: 1665
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TurboRFP-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-turboRFP-CSF1-EF1a-Hyg plasmid Description

pLV3-CMV-turboRFP-CSF1-EF1a-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-turboRFP-CSF1-EF1a-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcG CCACCATGAG CGAGCTGATC AAGGAGAACA TGCACATGAA GCTGTACATG 2341 GAGGGCACCG TGAACAACCA CCACTTCAAG TGCACATCCG AGGGCGAAGG CAAGCCCTAC 2401 GAGGGCACCC AGACCATGAA GATCAAGGTG GTCGAGGGCG GCCCTCTCCC CTTCGCCTTC 2461 GACATCCTGG CTACCAGCTT CATGTACGGC AGCAAAGCCT TCATCAACCA CACCCAGGGC 2521 ATCCCCGACT TCTTTAAGCA GTCCTTCCCT GAGGGCTTCA CATGGGAGAG AATCACCACA 2581 TACGAAGACG GGGGCGTGCT GACCGCTACC CAGGACACCA GCTTCCAGAA CGGCTGCATC 2641 ATCTACAACG TCAAGATCAA CGGGGTGAAC TTCCCATCCA ACGGCCCTGT GATGCAGAAG 2701 AAAACACGCG GCTGGGAGGC CAACACCGAG ATGCTGTACC CCGCTGACGG CGGCCTGAGA 2761 GGCCACAGCC AGATGGCCCT GAAGCTCGTG GGCGGGGGCT ACCTGCACTG CTCCTTCAAG 2821 ACCACATACA GATCCAAGAA ACCCGCTAAG AACCTCAAGA TGCCCGGCTT CCACTTCGTG 2881 GACCACAGAC TGGAAAGAAT CAAGGAGGCC GACAAAGAGA CCTACGTCGA GCAGCACGAG 2941 ATGGCTGTGG CCAAGTACTG CGACCTCCCT AGCAAACTGG GGCACAGAGG ATCCgGCGGC 3001 GGAGGGAGTG GAGGAGGGGG AAGTatgacc gcgccgggcg ccgccgggcg ctgccctccc 3061 acgacatggc tgggctccct gctgttgttg gtctgtctcc tggcgagcag gagtatcacc 3121 gaggaggtgt cggagtactg tagccacatg attgggagtg gacacctgca gtctctgcag 3181 cggctgattg acagtcagat ggagacctcg tgccaaatta catttgagtt tgtagaccag 3241 gaacagttga aagatccagt gtgctacctt aagaaggcat ttctcctggt acaagacata 3301 atggaggaca ccatgcgctt cagagataac acccccaatg ccatcgccat tgtgcagctg 3361 caggaactct ctttgaggct gaagagctgc ttcaccaagg attatgaaga gcatgacaag 3421 gcctgcgtcc gaactttcta tgagacacct ctccagttgc tggagaaggt caagaatgtc 3481 tttaatgaaa caaagaatct ccttgacaag gactggaata ttttcagcaa gaactgcaac 3541 aacagctttg ctgaatgctc cagccaagat gtggtgacca agcctgattg caactgcctg 3601 taccccaaag ccatccctag cagtgacccg gcctctgtct cccctcatca gcccctcgcc 3661 ccctccatgg cccctgtggc tggcttgacc tgggaggact ctgagggaac tgagggcagc 3721 tccctcttgc ctggtgagca gcccctgcac acagtggatc caggcagtgc caagcagcgg 3781 ccacccagga gcacctgcca gagctttgag ccgccagaga ccccagttgt caaggacagc 3841 accatcggtg gctcaccaca gcctcgcccc tctgtcgggg ccttcaaccc cgggatggag 3901 gatattcttg actctgcaat gggcactaat tgggtcccag aagaagcctc tggagaggcc 3961 agtgagattc ccgtacccca agggacagag ctttccccct ccaggccagg agggggcagc 4021 atgcagacag agcccgccag acccagcaac ttcctctcag catcttctcc actccctgca 4081 tcagcaaagg gccaacagcc ggcagatgta actggtaccg ccttgcccag ggtgggcccc 4141 gtgaggccca ctggccagga ctggaatcac accccccaga agacagacca tccatctgcc 4201 ctgctcagag accccccgga gccaggctct cccaggatct catcactgcg cccccagggc 4261 ctcagcaacc cctccaccct ctctgctcag ccacagcttt ccagaagcca ctcctcgggc 4321 agcgtgctgc cccttgggga gctggagggc aggaggagca ccagggatcg gaggagcccc 4381 gcagagccag aaggaggacc agcaagtgaa ggggcagcca ggcccctgcc ccgttttaac 4441 tccgttcctt tgactgacac aggccatgag aggcagtccg agggatcctt cagcccgcag 4501 ctccaggagt ctgtcttcca cctgctggtg cccagtgtca tcctggtctt gctggccgtc 4561 ggaggcctct tgttctacag gtggaggcgg cggagccatc aagagcctca gagagcggat 4621 tctcccttgg agcaaccaga gggcagcccc ctgactcagg atgacagaca ggtggaactg 4681 ccagtgtagG cggccgcaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 4741 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 4801 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 4861 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 4921 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 4981 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 5041 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 5101 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 5161 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 5221 tccaagctgt gaccggcgcc tacgtcgaca tgaaaaagcc tgaactcacc gcgacgtctg 5281 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg 5341 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa 5401 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg 5461 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca 5521 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg 5581 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga 5641 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca 5701 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca 5761 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag 5821 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca 5881 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca 5941 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc 6001 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc 6061 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg 6121 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc 6181 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc 6241 gacgccccag cactcgtccg agggcaaagg aataggtcga caatcaacct ctggattaca 6301 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 6361 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 6421 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 6481 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 6541 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 6601 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 6661 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga 6721 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 6781 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 6841 gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag 6901 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 6961 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 7021 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 7081 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 7141 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 7201 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 7261 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 7321 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 7381 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 7441 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 7501 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat 7561 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 7621 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 7681 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 7741 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 7801 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 7861 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 7921 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 7981 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 8041 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 8101 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 8161 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 8221 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 8281 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 8341 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 8401 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 8461 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 8521 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 8581 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 8641 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 8701 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 8761 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 8821 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 8881 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 8941 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 9001 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 9061 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 9121 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 9181 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 9241 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 9301 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 9361 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 9421 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 9481 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 9541 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 9601 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 9661 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 9721 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 9781 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 9841 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 9901 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag 9961 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 10021 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 10081 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 10141 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.