pLV3-CMV-ITGA6-EF1a-Hyg plasmi

pLV3-CMV-ITGA6-EF1a-Hyg plasmid

PVT111525

pLV3-CMV-ITGA6-EF1a-Hyg plasmid Information

Plasmid name: pLV3-CMV-ITGA6(human)-EF1a-Hyg
Alias: NM_000210.4;CD49f;ITGA6B;VLA-6
Gene lenght: 3219
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ITGA6-EF1a-Hyg plasmid Description

pLV3-CMV-ITGA6-EF1a-Hyg is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ITGA6-EF1a-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agaGCTAGCG ATATCCTCGA GGCCACCatg gccgccgccg ggcagctgtg cttgctctac 2341 ctgtcggcgg ggctcctgtc ccggctcggc gcagccttca acttggacac tcgggaggac 2401 aacgtgatcc ggaaatatgg agaccccggg agcctcttcg gcttctcgct ggccatgcac 2461 tggcaactgc agcccgagga caagcggctg ttgctcgtgg gggccccgcg ggcagaagcg 2521 cttccactgc agagagccaa cagaacggga gggctgtaca gctgcgacat caccgcccgg 2581 gggccatgca cgcggatcga gtttgataac gatgctgacc ccacgtcaga aagcaaggaa 2641 gatcagtgga tgggggtcac cgtccagagc caaggtccag ggggcaaggt cgtgacatgt 2701 gctcaccgat atgaaaaaag gcagcatgtt aatacgaagc aggaatcccg agacatcttt 2761 gggcggtgtt atgtcctgag tcagaatctc aggattgaag acgatatgga tgggggagat 2821 tggagctttt gtgatgggcg attgagaggc catgagaaat ttggctcttg ccagcaaggt 2881 gtagcagcta cttttactaa agactttcat tacattgtat ttggagcccc gggtacttat 2941 aactggaaag ggattgttcg tgtagagcaa aagaataaca ctttttttga catgaacatc 3001 tttgaagatg ggccttatga agttggtgga gagactgagc atgatgaaag tctcgttcct 3061 gttcctgcta acagttactt aggtttttct ttggactcag ggaaaggtat tgtttctaaa 3121 gatgagatca cttttgtatc tggtgctccc agagccaatc acagtggagc cgtggttttg 3181 ctgaagagag acatgaagtc tgcacatctc ctccctgagc acatattcga tggagaaggt 3241 ctggcctctt catttggcta tgatgtggcg gtggtggacc tcaacaagga tgggtggcaa 3301 gatatagtta ttggagcccc acagtatttt gatagagatg gagaagttgg aggtgcagtg 3361 tatgtctaca tgaaccagca aggcagatgg aataatgtga agccaattcg tcttaatgga 3421 accaaagatt ctatgtttgg cattgcagta aaaaatattg gagatattaa tcaagatggc 3481 tacccagata ttgcagttgg agctccgtat gatgacttgg gaaaggtttt tatctatcat 3541 ggatctgcaa atggaataaa taccaaacca acacaggttc tcaagggtat atcaccttat 3601 tttggatatt caattgctgg aaacatggac cttgatcgaa attcctaccc tgatgttgct 3661 gttggttccc tctcagattc agtaactatt ttcagatccc ggcctgtgat taatattcag 3721 aaaaccatca cagtaactcc taacagaatt gacctccgcc agaaaacagc gtgtggggcg 3781 cctagtggga tatgcctcca ggttaaatcc tgttttgaat atactgctaa ccccgctggt 3841 tataatcctt caatatcaat tgtgggcaca cttgaagctg aaaaagaaag aagaaaatct 3901 gggctatcct caagagttca gtttcgaaac caaggttctg agcccaaata tactcaagaa 3961 ctaactctga agaggcagaa acagaaagtg tgcatggagg aaaccctgtg gctacaggat 4021 aatatcagag ataaactgcg tcccattccc ataactgcct cagtggagat ccaagagcca 4081 agctctcgta ggcgagtgaa ttcacttcca gaagttcttc caattctgaa ttcagatgaa 4141 cccaagacag ctcatattga tgttcacttc ttaaaagagg gatgtggaga cgacaatgta 4201 tgtaacagca accttaaact agaatataaa ttttgcaccc gagaaggaaa tcaagacaaa 4261 ttttcttatt taccaattca aaaaggtgta ccagaactag ttctaaaaga tcagaaggat 4321 attgctttag aaataacagt gacaaacagc ccttccaacc caaggaatcc cacaaaagat 4381 ggcgatgacg cccatgaggc taaactgatt gcaacgtttc cagacacttt aacctattct 4441 gcatatagag aactgagggc tttccctgag aaacagttga gttgtgttgc caaccagaat 4501 ggctcgcaag ctgactgtga gctcggaaat ccttttaaaa gaaattcaaa tgtcactttt 4561 tatttggttt taagtacaac tgaagtcacc tttgacaccc cagatctgga tattaatctg 4621 aagttagaaa caacaagcaa tcaagataat ttggctccaa ttacagctaa agcaaaagtg 4681 gttattgaac tgcttttatc ggtctcggga gttgctaaac cttcccaggt gtattttgga 4741 ggtacagttg ttggcgagca agctatgaaa tctgaagatg aagtgggaag tttaatagag 4801 tatgaattca gggtaataaa cttaggtaaa cctcttacaa acctcggcac agcaaccttg 4861 aacattcagt ggccaaaaga aattagcaat gggaaatggt tgctttattt ggtgaaagta 4921 gaatccaaag gattggaaaa ggtaacttgt gagccacaaa aggagataaa ctccctgaac 4981 ctaacggagt ctcacaactc aagaaagaaa cgggaaatta ctgaaaaaca gatagatgat 5041 aacagaaaat tttctttatt tgctgaaaga aaataccaga ctcttaactg tagcgtgaac 5101 gtgaactgtg tgaacatcag atgcccgctg cgggggctgg acagcaaggc gtctcttatt 5161 ttgcgctcga ggttatggaa cagcacattt ctagaggaat attccaaact gaactacttg 5221 gacattctca tgcgagcctt cattgatgtg actgctgctg ccgaaaatat caggctgcca 5281 aatgcaggca ctcaggttcg agtgactgtg tttccctcaa agactgtagc tcagtattcg 5341 ggagtacctt ggtggatcat cctagtggct attctcgctg ggatcttgat gcttgcttta 5401 ttagtgttta tactatggaa gtgtggtttc ttcaagagaa ataagaaaga tcattatgat 5461 gccacatatc acaaggctga gatccatgct cagccatctg ataaagagag gcttacttct 5521 gatgcaTAgg atccgcggcc gcaaggatct gcgatcgctc cggtgcccgt cagtgggcag 5581 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg 5641 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt 5701 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc 5761 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac 5821 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc 5881 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga 5941 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc 6001 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt 6061 acagatccaa gctgtgaccg gcgcctacgt cgacatgaaa aagcctgaac tcaccgcgac 6121 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc 6181 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg 6241 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc 6301 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta 6361 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc 6421 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca 6481 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga 6541 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac 6601 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc 6661 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg 6721 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt 6781 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt 6841 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat 6901 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc 6961 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat 7021 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg 7081 aaaccgacgc cccagcactc gtccgagggc aaaggaatag gtcgacaatc aacctctgga 7141 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 7201 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 7261 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 7321 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 7381 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 7441 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 7501 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac 7561 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 7621 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 7681 gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt 7741 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 7801 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 7861 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 7921 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 7981 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 8041 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 8101 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 8161 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 8221 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 8281 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 8341 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta 8401 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 8461 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 8521 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 8581 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 8641 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 8701 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 8761 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 8821 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 8881 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 8941 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 9001 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 9061 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 9121 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 9181 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 9241 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 9301 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 9361 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 9421 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 9481 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 9541 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 9601 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 9661 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 9721 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 9781 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 9841 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 9901 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 9961 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 10021 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 10081 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 10141 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 10201 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 10261 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 10321 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 10381 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 10441 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 10501 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 10561 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 10621 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 10681 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 10741 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 10801 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 10861 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 10921 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 10981 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.