pCMV-BMP2K-3×HA-Neo plasmid

pCMV-BMP2K-3×HA-Neo plasmid

PVT108108

pCMV-BMP2K-3×HA-Neo plasmid Information

Plasmid name: pCMV-BMP2K(human)-3×HA-Neo
Alias: NM_001419799.1;BIKE; HRIHFB2017
Gene lenght: 3372
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-BMP2K-3×HA-Neo plasmid Description

pCMV-BMP2K-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-BMP2K-3×HA-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CACTAGTCCA GTGTGGTGGA ATTCGCCACC atgaagaagt tctctcggat gcccaagtcg 961 gagggcggca gcggcggcgg agcggcgggt ggcggggctg gcggggccgg ggccggggcc 1021 ggctgcggct ccggcggctc gtccgtgggg gtccgggtgt tcgcggtcgg ccgccaccag 1081 gtcaccctgg aagagtcgct ggccgaaggt ggattctcca cagttttcct cgtgcgtact 1141 cacggtggaa tccgatgtgc attgaagcga atgtatgtca ataacatgcc agacctcaat 1201 gtttgtaaaa gggaaattac aattatgaaa gagctatctg gtcacaaaaa tattgtgggc 1261 tatttggact gtgctgttaa ttcaattagt gataatgtat gggaagtcct tatcttaatg 1321 gaatattgtc gagctggaca ggtagtgaat caaatgaata agaagctaca gacgggtttt 1381 acagaaccag aagtgttaca gatattctgt gatacctgtg aagctgttgc aaggttgcat 1441 cagtgtaaga ctccaataat tcaccgggat ctgaaggtag aaaatatttt gttgaatgat 1501 ggtgggaact atgtactttg tgactttggc agtgccacta ataaatttct taatcctcaa 1561 aaagatggag ttaatgtagt agaagaagaa attaaaaagt atacaactct gtcatacaga 1621 gcccctgaaa tgatcaacct ttatggaggg aaacccatca ccaccaaggc tgatatctgg 1681 gcactgggat gtctactcta taaactttgt ttcttcactc ttccttttgg tgagagtcag 1741 gttgctatct gtgatggcaa cttcaccatc ccagacaatt ctcgttactc ccgtaacata 1801 cattgcttaa taaggttcat gcttgaacca gatccggaac atagacctga tatatttcaa 1861 gtgtcatatt ttgcatttaa atttgccaaa aaggattgtc cagtctccaa catcaataat 1921 tcttctattc cttcagctct tcctgaaccg atgactgcta gtgaagcagc tgctaggaaa 1981 agccaaataa aagccagaat aacagatacc attggaccaa cagaaacctc aattgcacca 2041 agacaaagac caaaggccaa ctctgctact actgccactc ccagtgtgct gaccattcaa 2101 agttcagcaa cacctgttaa agtccttgct cctggtgaat tcggtaacca tagaccaaaa 2161 ggggcactaa gacctggaaa tggccctgaa attttattgg gtcagggacc tcctcagcag 2221 ccgccacagc agcatagagt actccagcaa ctacagcagg gagattggag attacagcaa 2281 ctccatttac agcatcgtca tcctcaccag cagcagcagc agcagcagca gcaacagcaa 2341 cagcagcagc agcaacagca acagcagcag cagcagcagc agcagcagca ccaccaccac 2401 caccaccacc acctacttca agatgcttat atgcagcagt atcaacatgc aacacagcag 2461 caacagatgc ttcaacaaca atttttaatg cattcggtat atcaaccaca accttctgca 2521 tcacagtatc ctacaatgat gccgcagtat cagcaggctt tctttcaaca gcagatgcta 2581 gctcaacatc agccgtctca acaacaggca tcacctgaat atcttacctc ccctcaagag 2641 ttctcaccag ccttagtttc ctacacttca tcacttccag ctcaggttgg aaccataatg 2701 gactcctcct atagtgccaa taggtcagtt gctgataaag aggccattgc aaatttcaca 2761 aatcagaaga acatcagcaa tccacctgat atgtcagggt ggaatccttt tggagaggat 2821 aatttctcta agttaacaga agaggaacta ttggacagag aatttgacct tctaagatca 2881 agttctcctg aaaagaaagc tgaacattca tctataaatc aagaaaatgg cactgcaaac 2941 cctatcaaga acggtaaaac aagtccagca tctaaagatc agcggactgg aaagaaaacc 3001 tcagtacagg gtcaagtgca aaaggggaat gatgaatctg aaagtgattt tgaatcagat 3061 cccccttctc ctaagagcag tgaagaggaa gagcaagatg atgaagaagt tcttcagggg 3121 gaacaaggag attttaatga tgatgatact gaaccagaaa atctgggtca taggcctctc 3181 ctcatggatt ctgaagatga ggaagaagag gagaaacata gctctgattc tgattatgag 3241 caggctaaag caaagtacag tgacatgagc tctgtctaca gagacagatc tggcagtgga 3301 ccaacccaag atcttaatac aatactcctc acctcagccc aattatcctc tgatgttgca 3361 gtggagactc ccaaacagga gtttgatgta tttggcgctg tccccttctt tgcagtgcgt 3421 gctcaacagc cccagcaaga aaagaatgaa aagaacctcc ctcaacacag gtttcctgct 3481 gcaggactgg agcaggagga atttgatgta ttcacaaagg cgccttttag caagaaggtg 3541 aatgtacaag aatgccatgc agtggggcct gaggcacata ctatccctgg ttatcccaaa 3601 agtgtagatg tatttggctc cactccattt cagcccttcc tcacatcaac aagtaaaagt 3661 gaaagcaatg aggacctttt tgggcttgtg ccctttgatg aaataacggg gagccagcag 3721 caaaaagtca aacagcgcag cttacagaaa ctgtcctctc gccaaaggcg cacaaagcag 3781 gatatgtcca aaagtaatgg gaagcggcat catggcacgc caactagcac aaagaagact 3841 ttgaagccta cctatcgcac tccagagagg gctcgcaggc acaaaaaagt gggccgccga 3901 gactctcaaa gtagcaatga atttttaacc atctcagact ccaaggagaa cattagtgtt 3961 gcactgactg atgggaaaga tagggggaat gtcttacaac ctgaggagag cctgttggac 4021 cccttcggtg ccaagccctt ccattctcca gacctgtcat ggcaccctcc acatcagggc 4081 ctgagcgaca tccgtgctga tcacaatact gtcctgccag ggcggccaag acaaaattca 4141 ctacatgggt cattccatag tgcagatgta ttgaaaatgg atgattttgg tgccgtgccc 4201 tttacagaac ttgtggtgca aagcatcact ccacatcagt cccaacagtc ccaaccagtc 4261 gaattagacc catttggtgc tgctccattt ccttctaaac agCTCGAGTA CCCATACGAC 4321 GTACCTGATT ATGCAACTTA TCCTTATGAT GTACCTGATT ATGCTAGCTA TCCTTATGAT 4381 GTCCCAGACT ACGCAtgaGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT 4441 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC 4501 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT 4561 CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT 4621 AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG 4681 GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 4741 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 4801 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 4861 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 4921 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 4981 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 5041 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 5101 CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG 5161 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG 5221 CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 5281 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC 5341 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG 5401 AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG 5461 GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG 5521 GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT 5581 GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG 5641 CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG 5701 GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG 5761 TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG 5821 GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA 5881 TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC 5941 ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC 6001 AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA 6061 AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA 6121 ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG 6181 CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG 6241 AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG 6301 CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA 6361 CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG 6421 GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT 6481 CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA 6541 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG 6601 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG 6661 CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC 6721 ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA 6781 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA 6841 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC 6901 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC 6961 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 7021 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 7081 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 7141 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 7201 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 7261 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 7321 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 7381 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 7441 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 7501 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 7561 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT 7621 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 7681 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 7741 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 7801 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 7861 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 7921 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC 7981 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 8041 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 8101 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT 8161 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 8221 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 8281 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 8341 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 8401 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA 8461 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 8521 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 8581 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 8641 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 8701 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 8761 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 8821 ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.