pCMV-BMP2K-3×FLAG-Neo plasmid

pCMV-BMP2K-3×FLAG-Neo plasmid

PVT108061

pCMV-BMP2K-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-BMP2K(human)-3×FLAG-Neo
Alias: NM_001419799.1;BIKE; HRIHFB2017
Gene lenght: 3372
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-BMP2K-3×FLAG-Neo plasmid Description

pCMV-BMP2K-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-BMP2K-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgaa gaagttctct cggatgccca 781 agtcggaggg cggcagcggc ggcggagcgg cgggtggcgg ggctggcggg gccggggccg 841 gggccggctg cggctccggc ggctcgtccg tgggggtccg ggtgttcgcg gtcggccgcc 901 accaggtcac cctggaagag tcgctggccg aaggtggatt ctccacagtt ttcctcgtgc 961 gtactcacgg tggaatccga tgtgcattga agcgaatgta tgtcaataac atgccagacc 1021 tcaatgtttg taaaagggaa attacaatta tgaaagagct atctggtcac aaaaatattg 1081 tgggctattt ggactgtgct gttaattcaa ttagtgataa tgtatgggaa gtccttatct 1141 taatggaata ttgtcgagct ggacaggtag tgaatcaaat gaataagaag ctacagacgg 1201 gttttacaga accagaagtg ttacagatat tctgtgatac ctgtgaagct gttgcaaggt 1261 tgcatcagtg taagactcca ataattcacc gggatctgaa ggtagaaaat attttgttga 1321 atgatggtgg gaactatgta ctttgtgact ttggcagtgc cactaataaa tttcttaatc 1381 ctcaaaaaga tggagttaat gtagtagaag aagaaattaa aaagtataca actctgtcat 1441 acagagcccc tgaaatgatc aacctttatg gagggaaacc catcaccacc aaggctgata 1501 tctgggcact gggatgtcta ctctataaac tttgtttctt cactcttcct tttggtgaga 1561 gtcaggttgc tatctgtgat ggcaacttca ccatcccaga caattctcgt tactcccgta 1621 acatacattg cttaataagg ttcatgcttg aaccagatcc ggaacataga cctgatatat 1681 ttcaagtgtc atattttgca tttaaatttg ccaaaaagga ttgtccagtc tccaacatca 1741 ataattcttc tattccttca gctcttcctg aaccgatgac tgctagtgaa gcagctgcta 1801 ggaaaagcca aataaaagcc agaataacag ataccattgg accaacagaa acctcaattg 1861 caccaagaca aagaccaaag gccaactctg ctactactgc cactcccagt gtgctgacca 1921 ttcaaagttc agcaacacct gttaaagtcc ttgctcctgg tgaattcggt aaccatagac 1981 caaaaggggc actaagacct ggaaatggcc ctgaaatttt attgggtcag ggacctcctc 2041 agcagccgcc acagcagcat agagtactcc agcaactaca gcagggagat tggagattac 2101 agcaactcca tttacagcat cgtcatcctc accagcagca gcagcagcag cagcagcaac 2161 agcaacagca gcagcagcaa cagcaacagc agcagcagca gcagcagcag cagcaccacc 2221 accaccacca ccaccaccta cttcaagatg cttatatgca gcagtatcaa catgcaacac 2281 agcagcaaca gatgcttcaa caacaatttt taatgcattc ggtatatcaa ccacaacctt 2341 ctgcatcaca gtatcctaca atgatgccgc agtatcagca ggctttcttt caacagcaga 2401 tgctagctca acatcagccg tctcaacaac aggcatcacc tgaatatctt acctcccctc 2461 aagagttctc accagcctta gtttcctaca cttcatcact tccagctcag gttggaacca 2521 taatggactc ctcctatagt gccaataggt cagttgctga taaagaggcc attgcaaatt 2581 tcacaaatca gaagaacatc agcaatccac ctgatatgtc agggtggaat ccttttggag 2641 aggataattt ctctaagtta acagaagagg aactattgga cagagaattt gaccttctaa 2701 gatcaagttc tcctgaaaag aaagctgaac attcatctat aaatcaagaa aatggcactg 2761 caaaccctat caagaacggt aaaacaagtc cagcatctaa agatcagcgg actggaaaga 2821 aaacctcagt acagggtcaa gtgcaaaagg ggaatgatga atctgaaagt gattttgaat 2881 cagatccccc ttctcctaag agcagtgaag aggaagagca agatgatgaa gaagttcttc 2941 agggggaaca aggagatttt aatgatgatg atactgaacc agaaaatctg ggtcataggc 3001 ctctcctcat ggattctgaa gatgaggaag aagaggagaa acatagctct gattctgatt 3061 atgagcaggc taaagcaaag tacagtgaca tgagctctgt ctacagagac agatctggca 3121 gtggaccaac ccaagatctt aatacaatac tcctcacctc agcccaatta tcctctgatg 3181 ttgcagtgga gactcccaaa caggagtttg atgtatttgg cgctgtcccc ttctttgcag 3241 tgcgtgctca acagccccag caagaaaaga atgaaaagaa cctccctcaa cacaggtttc 3301 ctgctgcagg actggagcag gaggaatttg atgtattcac aaaggcgcct tttagcaaga 3361 aggtgaatgt acaagaatgc catgcagtgg ggcctgaggc acatactatc cctggttatc 3421 ccaaaagtgt agatgtattt ggctccactc catttcagcc cttcctcaca tcaacaagta 3481 aaagtgaaag caatgaggac ctttttgggc ttgtgccctt tgatgaaata acggggagcc 3541 agcagcaaaa agtcaaacag cgcagcttac agaaactgtc ctctcgccaa aggcgcacaa 3601 agcaggatat gtccaaaagt aatgggaagc ggcatcatgg cacgccaact agcacaaaga 3661 agactttgaa gcctacctat cgcactccag agagggctcg caggcacaaa aaagtgggcc 3721 gccgagactc tcaaagtagc aatgaatttt taaccatctc agactccaag gagaacatta 3781 gtgttgcact gactgatggg aaagataggg ggaatgtctt acaacctgag gagagcctgt 3841 tggacccctt cggtgccaag cccttccatt ctccagacct gtcatggcac cctccacatc 3901 agggcctgag cgacatccgt gctgatcaca atactgtcct gccagggcgg ccaagacaaa 3961 attcactaca tgggtcattc catagtgcag atgtattgaa aatggatgat tttggtgccg 4021 tgccctttac agaacttgtg gtgcaaagca tcactccaca tcagtcccaa cagtcccaac 4081 cagtcgaatt agacccattt ggtgctgctc catttccttc taaacagGGA TCCGACTACA 4141 AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGT 4201 AGTGAGGGCC CGATATCTCG ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC 4261 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT 4321 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT 4381 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT 4441 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG 4501 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG 4561 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT 4621 GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT 4681 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC 4741 TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC 4801 CGCCTCCCCG CCTGGAAACG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA 4861 GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGGTG TTGGGTCGTT 4921 TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC 4981 CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG 5041 TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CAGATCTGCG 5101 CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG 5161 TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT 5221 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG 5281 GGGCATCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA 5341 TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC 5401 GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC 5461 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGGGG ATTTCGGCCT ATTGGTTAAA 5521 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA 5581 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 5641 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 5701 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 5761 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 5821 GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 5881 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 5941 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 6001 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 6061 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 6121 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 6181 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 6241 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 6301 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 6361 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 6421 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 6481 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 6541 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 6601 GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 6661 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 6721 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGCGA 6781 AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT 6841 CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG 6901 CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG 6961 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC 7021 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC 7081 TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT 7141 CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG 7201 AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT 7261 GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG 7321 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC 7381 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 7441 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 7501 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 7561 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 7621 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 7681 GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 7741 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 7801 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 7861 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 7921 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 7981 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 8041 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 8101 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 8161 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 8221 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT 8281 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC 8341 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT 8401 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG 8461 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG 8521 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC 8581 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA 8641 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG 8701 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC 8761 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA 8821 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC 8881 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG 8941 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC 9001 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC 9061 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT 9121 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG 9181 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC 9241 CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTCGACT 9301 CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG 9361 TTGGAGGTCG CTGAGTAGTG CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC 9421 GACAATTGCA TGAAGAATCT GCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.