pCMV-GATAD2B-3×FLAG-SP6-Neo pl

pCMV-GATAD2B-3×FLAG-SP6-Neo plasmid

PVT108009

pCMV-GATAD2B-3×FLAG-SP6-Neo plasmid Information

Plasmid name: pCMV-GATAD2B(human)-3×FLAG-SP6-Neo
Alias: NM_020699.4;GANDS; MRD18; P66beta; p68
Gene lenght: 1779
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-GATAD2B-3×FLAG-SP6-Neo plasmid Description

pCMV-GATAD2B-3×FLAG-SP6-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-GATAD2B-3×FLAG-SP6-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttGCCACC 901 atggatagaa tgacagaaga tgctcttcgc ttgaatctgt tgaagcggag cttggaccca 961 gcagatgagc gagatgatgt cctggcaaag cgactcaaaa tggaggggca tgaggccatg 1021 gaacgtctga aaatgttggc attgctcaaa aggaaggatt tggcaaatct tgaggtgcca 1081 catgagttac ccaccaaaca ggatggcagt ggtgtcaagg gctatgaaga aaaacttaac 1141 gggaatctca ggcctcatgg agacaacagg actgctggaa ggccaggcaa agaaaacatc 1201 aatgatgagc ctgtggatat gagtgctaga cggagtgagc cagagcgagg aaggctaact 1261 ccctcaccag acatcattgt tttgtctgac aatgaggctt ccagtccccg ttccagttcc 1321 agaatggaag aaagactcaa agcagccaac ttagagatgt ttaaggggaa aggcattgag 1381 gagcggcagc agcttatcaa gcagctgagg gatgagctac gattggaaga agcccgactg 1441 gtcctgttaa agaaactgag acagagtcag ctacagaaag agaatgtggt ccagaagact 1501 ccagttgtac agaatgcagc atctattgtt cagccatctc ctgcccatgt gggacagcag 1561 ggcctatcta agcttccctc tcggcctggg gcccaagggg ttgaacctca aaatttgaga 1621 acattacagg gtcacagtgt catccgttca gctaccaata ccacccttcc acacatgttg 1681 atgtctcaac gtgttattgc accaaaccca gcccagctac agggtcagcg gggcccgcct 1741 aagcctggcc ttgtacgcac cacaacaccc aacatgaatc ccgccatcaa ttatcaaccg 1801 cagtcaagtt cttctgttcc atgtcagcgt acaacatcct ctgccatcta tatgaacctt 1861 gcttctcata tccagccagg gacggtgaac agagtgtcct cgccacttcc tagccccagc 1921 gccatgactg atgctgccaa ctcacaggct gcagccaaat tggctcttcg caaacagctg 1981 gaaaagacac tcctggagat cccaccccct aaacctcctg ctcccttact tcatttcttg 2041 cctagtgcag ccaatagcga gttcatctac atggtaggct tggaagaagt cgtacagagt 2101 gtcattgaca gccaaggcaa aagctgtgcc tcacttctgc gggttgaacc ctttgtatgt 2161 gcccagtgcc gcacagattt cacccctcac tggaagcaag aaaagaatgg taagattcta 2221 tgtgagcagt gtatgacctc caaccagaaa aaggctctaa aagctgaaca caccaaccgg 2281 ctgaaaaatg catttgtgaa agccctacag caggaacagg aaattgaaca gcgattacag 2341 cagcaggcag ccctctcccc cactacggct ccagctgtgt ccagtgtcag taaacaagag 2401 accatcatga gacatcatac gcttcggcag gctccacagc cccagagcag cctccagcgt 2461 ggcataccca catctgcccg ctccatgctt tcaaactttg cacaggcacc ccagttgtct 2521 gtgccaggtg gcctccttgg tatgccaggt gtcaacattg catacttgaa tactggcatc 2581 ggaggacaca aaggccccag tttggcagac cgacagcgtg aatacctttt agacatgatc 2641 cctccccggt ctatatcgca gtccatcagt ggacagaaaG GATCCGACTA CAAAGACCAT 2701 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GTAGggatcc 2761 actagtaacg gccgccagtg tgctggaatt ctgcagatat ccatcacact ggcggccgct 2821 cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat 2881 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 2941 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 3001 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 3061 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 3121 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat 3181 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 3241 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 3301 aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc 3361 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 3421 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 3481 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg 3541 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa 3601 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa 3661 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 3721 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 3781 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 3841 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 3901 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 3961 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 4021 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 4081 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 4141 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 4201 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 4261 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 4321 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 4381 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 4441 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 4501 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 4561 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 4621 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 4681 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 4741 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 4801 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 4861 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 4921 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 4981 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 5041 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 5101 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 5161 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 5221 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 5281 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 5341 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 5401 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 5461 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 5521 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 5581 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 5641 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 5701 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 5761 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 5821 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 5881 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 5941 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 6001 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 6061 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 6121 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 6181 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 6241 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 6301 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 6361 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 6421 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 6481 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 6541 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 6601 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 6661 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 6721 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 6781 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 6841 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 6901 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 6961 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 7021 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 7081 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 7141 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 7201 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 7261 gcgcacattt ccccgaaaag tgccacctga cgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.