pCMV-SLC1A6-P2A-mCherry-Neo pl

pCMV-SLC1A6-P2A-mCherry-Neo plasmid

PVT107121

pCMV-SLC1A6-P2A-mCherry-Neo plasmid Information

Plasmid name: pCMV-SLC1A6(human)-P2A-mCherry-Neo
Alias: NM_005071.3;EAAT4
Gene lenght: 1692
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SLC1A6-P2A-mCherry-Neo plasmid Description

pCMV-SLC1A6-P2A-mCherry-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-SLC1A6-P2A-mCherry-Neo plasmid Sequence

1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagc tagcgtttaa acttaagctt ggtaccgagc tcggatccaG CCACCatgag 781 cagccatggc aacagcctgt tcctgcggga gagcggccag cggctgggcc gggtgggctg 841 gctgcagcgg ctgcaggaaa gcctgcagca gagagcactg cgcacgcgcc tgcgcctgca 901 gaccatgacc ctcgagcacg tgctgcgctt cctgcgccga aacgccttca ttctgctgac 961 ggtcagcgcc gtggtcattg gggtcagcct ggcctttgcc ctgcgcccat atcagctcac 1021 ctaccgccag atcaagtact tctcttttcc tggagagctt ctgatgagga tgctgcagat 1081 gctggtgtta cctctcattg tctccagcct ggtcacaggt atggcatccc tggacaacaa 1141 ggccacgggg cggatgggga tgcgggcagc tgtgtactac atggtgacca ccatcatcgc 1201 ggtcttcatc ggcatcctca tggtcaccat catccatccc gggaagggct ccaaggaggg 1261 gctgcaccgg gagggccgga tcgagaccat ccccacagct gatgccttca tggacctgat 1321 cagaaatatg tttccaccaa accttgtgga ggcctgcttc aaacagttca agacgcagta 1381 cagcacgagg gtggtaacca ggaccatggt gaggacagag aacgggtctg agccgggtgc 1441 ctccatgcct cctccattct cagtggagaa cggaaccagc ttcctggaaa atgtcactcg 1501 ggccttgggt accctgcagg agatgctgag ctttgaggag actgtacccg tgcctggctc 1561 cgccaatggc atcaacgccc tgggcctcgt ggtcttctct gtggcctttg ggctggtcat 1621 tggtggcatg aaacacaagg gcagagtcct cagggacttc ttcgacagcc tcaatgaggc 1681 tattatgagg ctggtgggca tcattatctg gtatgcacct gtgggcatcc tgttcctgat 1741 tgctgggaag attctggaga tggaagacat ggccgtcctg gggggtcagc tgggcatgta 1801 caccctgacc gtcatcgtgg gcctgttcct ccatgccggc attgtccttc ccctcatcta 1861 cttcctcgtc actcaccgga accccttccc cttcattggg ggcatgctac aagccctcat 1921 caccgctatg ggcacgtctt ccagctcggc aacgctgccc atcaccttcc gctgcctgga 1981 ggagggcctg ggtgtggacc gccgcatcac caggttcgtc ctgcccgtgg gcgccacggt 2041 caacatggat ggcactgccc tctacgaggc cctggctgcc atcttcattg ctcaagttaa 2101 caactacgag ctcaacctgg gtcagatcac aaccatcagc atcacggcca cagcagccag 2161 tgttggggct gctggcatcc cccaggcggg tctggtcacc atggtcattg tgcttacgtc 2221 ggtcggcttg cccacggaag acatcacgct catcattgcc gtggactggt tccttgaccg 2281 gcttcgcaca atgaccaacg tactggggga ctcaattgga gcggccgtca tcgagcactt 2341 gtctcagcgg gagctggagc ttcaggaagc tgagcttacc ctccccagcc tggggaaacc 2401 ctacaagtcc ctcatggcac aggagaaggg ggcatcccgg ggacggggag gcaacgagag 2461 tgctatggaa ttcggaagcg gagctactaa cttcagcctg ctgaagcagg ctggcgacgt 2521 ggaggagaac cctggaccta tggtgagcaa gggcgaggag gataacatgg ccatcatcaa 2581 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat 2641 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac 2701 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc 2761 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga 2821 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca 2881 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt 2941 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg 3001 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga 3061 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct 3121 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac 3181 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct 3241 gtacaagtaa ctcgagtcta gagggcccct taagggttcg atccctaccg gttagtaatg 3301 agtttgatat ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 3361 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 3421 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 3481 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 3541 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 3601 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 3661 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 3721 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 3781 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 3841 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 3901 cccgcctgga aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc 3961 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca 4021 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg 4081 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg 4141 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg 4201 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 4261 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 4321 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat 4381 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 4441 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 4501 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 4561 ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga 4621 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 4681 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 4741 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 4801 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 4861 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 4921 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 4981 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca 5041 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct 5101 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 5161 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 5221 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc 5281 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 5341 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 5401 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 5461 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 5521 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 5581 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 5641 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 5701 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 5761 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 5821 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gcgaaatgac 5881 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 5941 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 6001 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa 6061 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 6121 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta 6181 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 6241 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 6301 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 6361 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 6421 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 6481 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 6541 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 6601 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 6661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 6721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 6781 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 6841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 6901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 7021 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 7081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 7201 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 7261 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 7321 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 7381 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 7441 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 7501 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 7561 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 7621 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 7681 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 7741 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 7801 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 7861 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 7921 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 7981 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 8041 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 8101 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 8161 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 8221 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 8281 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 8341 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtc gactctcagt 8401 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag 8461 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat 8521 tgcatgaaga atctgcttag g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.