pLV3-CMV-SMARCA4-TurboID-NLS-3

pLV3-CMV-SMARCA4-TurboID-NLS-3×FLAG-Puro plasmid

PVT104023

pLV3-CMV-SMARCA4-TurboID-NLS-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-SMARCA4(human)-TurboID-NLS-3×FLAG-Puro
Alias: NM_003072.5,SMARCA4/BAF190/BAF190A/BRG1/CSS4/hSNF2b/MRD16/RTPS2/SNF2/SNF2-beta/SNF2L4/SNF2LB/SWI2
Gene lenght: 4941
Host: Mammalian cells,lentivirus
Use(s): Protein Expression,subcellular localization
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SMARCA4-TurboID-NLS-3×FLAG-Puro plasmid Description

pLV3-CMV-SMARCA4-TurboID-NLS-3×FLAG-Puro is a plasmid suitable for protein expression and subcellular localization in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SMARCA4-TurboID-NLS-3×FLAG-Puro plasmid Sequence

1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct 4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 4321 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 4381 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 4441 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 4501 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 4561 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 4621 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc 4681 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact ctagaggatc 4741 cGCCACCATG TCCACTCCAG ACCCACCCCT GGGCGGAACT CCTCGGCCAG GTCCTTCCCC 4801 GGGCCCTGGC CCTTCCCCTG GAGCCATGCT GGGCCCTAGC CCGGGTCCCT CGCCGGGCTC 4861 CGCCCACAGC ATGATGGGGC CCAGCCCAGG GCCGCCCTCA GCAGGACACC CCATCCCCAC 4921 CCAGGGGCCT GGAGGGTACC CTCAGGACAA CATGCACCAG ATGCACAAGC CCATGGAGTC 4981 CATGCATGAG AAGGGCATGT CGGACGACCC GCGCTACAAC CAGATGAAAG GAATGGGGAT 5041 GCGGTCAGGG GGCCATGCTG GGATGGGGCC CCCGCCCAGC CCCATGGACC AGCACTCCCA 5101 AGGTTACCCC TCGCCCCTGG GTGGCTCTGA GCATGCCTCT AGTCCAGTTC CAGCCAGTGG 5161 CCCGTCTTCG GGGCCCCAGA TGTCTTCCGG GCCAGGAGGT GCCCCGCTGG ATGGTGCTGA 5221 CCCCCAGGCC TTGGGGCAGC AGAACCGGGG CCCAACCCCA TTTAACCAGA ACCAGCTGCA 5281 CCAGCTCAGA GCTCAGATCA TGGCCTACAA GATGCTGGCC AGGGGGCAGC CCCTCCCCGA 5341 CCACCTGCAG ATGGCGGTGC AGGGCAAGCG GCCGATGCCC GGGATGCAGC AGCAGATGCC 5401 AACGCTACCT CCACCCTCGG TGTCCGCAAC AGGACCCGGC CCTGGCCCTG GCCCTGGCCC 5461 CGGCCCGGGT CCCGGCCCGG CACCTCCAAA TTACAGCAGG CCTCATGGTA TGGGAGGGCC 5521 CAACATGCCT CCCCCAGGAC CCTCGGGCGT GCCCCCCGGG ATGCCAGGCC AGCCTCCTGG 5581 AGGGCCTCCC AAGCCCTGGC CTGAAGGACC CATGGCGAAT GCTGCTGCCC CCACGAGCAC 5641 CCCTCAGAAG CTGATTCCCC CGCAGCCAAC GGGCCGCCCT TCCCCCGCGC CCCCTGCCGT 5701 CCCACCCGCC GCCTCGCCCG TGATGCCACC GCAGACCCAG TCCCCCGGGC AGCCGGCCCA 5761 GCCCGCGCCC ATGGTGCCAC TGCACCAGAA GCAGAGCCGC ATCACCCCCA TCCAGAAGCC 5821 GCGGGGCCTC GACCCTGTGG AGATCCTGCA GGAGCGCGAG TACAGGCTGC AGGCTCGCAT 5881 CGCACACCGA ATTCAGGAAC TTGAAAACCT TCCCGGGTCC CTGGCCGGGG ATTTGCGAAC 5941 CAAAGCGACC ATTGAGCTCA AGGCCCTCAG GCTGCTGAAC TTCCAGAGGC AGCTGCGCCA 6001 GGAGGTGGTG GTGTGCATGC GGAGGGACAC AGCGCTGGAG ACAGCCCTCA ATGCTAAGGC 6061 CTACAAGCGC AGCAAGCGCC AGTCCCTGCG CGAGGCCCGC ATCACTGAGA AGCTGGAGAA 6121 GCAGCAGAAG ATCGAGCAGG AGCGCAAGCG CCGGCAGAAG CACCAGGAAT ACCTCAATAG 6181 CATTCTCCAG CATGCCAAGG ATTTCAAGGA ATATCACAGA TCCGTCACAG GCAAAATCCA 6241 GAAGCTGACC AAGGCAGTGG CCACGTACCA TGCCAACACG GAGCGGGAGC AGAAGAAAGA 6301 GAACGAGCGG ATCGAGAAGG AGCGCATGCG GAGGCTCATG GCTGAAGATG AGGAGGGGTA 6361 CCGCAAGCTC ATCGACCAGA AGAAGGACAA GCGCCTGGCC TACCTCTTGC AGCAGACAGA 6421 CGAGTACGTG GCTAACCTCA CGGAGCTGGT GCGGCAGCAC AAGGCTGCCC AGGTCGCCAA 6481 GGAGAAAAAG AAGAAAAAGA AAAAGAAGAA GGCAGAAAAT GCAGAAGGAC AGACGCCTGC 6541 CATTGGGCCG GATGGCGAGC CTCTGGACGA GACCAGCCAG ATGAGCGACC TCCCGGTGAA 6601 GGTGATCCAC GTGGAGAGTG GGAAGATCCT CACAGGCACA GATGCCCCCA AAGCCGGGCA 6661 GCTGGAGGCC TGGCTCGAGA TGAACCCGGG GTATGAAGTA GCTCCGAGGT CTGATAGTGA 6721 AGAAAGTGGC TCAGAAGAAG AGGAAGAGGA GGAGGAGGAA GAGCAGCCGC AGGCAGCACA 6781 GCCTCCCACC CTGCCCGTGG AGGAGAAGAA GAAGATTCCA GATCCAGACA GCGATGACGT 6841 CTCTGAGGTG GACGCGCGGC ACATCATTGA GAATGCCAAG CAAGATGTCG ATGATGAATA 6901 TGGCGTGTCC CAGGCCCTTG CACGTGGCCT GCAGTCCTAC TATGCCGTGG CCCATGCTGT 6961 CACTGAGAGA GTGGACAAGC AGTCAGCGCT TATGGTCAAT GGTGTCCTCA AACAGTACCA 7021 GATCAAAGGT TTGGAGTGGC TGGTGTCCCT GTACAACAAC AACCTGAACG GCATCCTGGC 7081 CGACGAGATG GGCCTGGGGA AGACCATCCA GACCATCGCG CTCATCACGT ACCTCATGGA 7141 GCACAAACGC ATCAATGGGC CCTTCCTCAT CATCGTGCCT CTCTCAACGC TGTCCAACTG 7201 GGCGTACGAG TTTGACAAGT GGGCCCCCTC CGTGGTGAAG GTGTCTTACA AGGGATCCCC 7261 AGCAGCAAGA CGGGCCTTTG TCCCCCAGCT CCGGAGTGGG AAGTTCAACG TCTTGCTGAC 7321 GACGTACGAG TACATCATCA AAGACAAGCA CATCCTCGCC AAGATCCGTT GGAAGTACAT 7381 GATTGTGGAC GAAGGTCACC GCATGAAGAA CCACCACTGC AAGCTGACGC AGGTGCTCAA 7441 CACGCACTAT GTGGCACCCC GCCGCCTGCT GCTGACGGGC ACACCGCTGC AGAACAAGCT 7501 TCCCGAGCTC TGGGCGCTGC TCAACTTCCT GCTGCCCACC ATCTTCAAGA GCTGCAGCAC 7561 CTTCGAGCAG TGGTTTAACG CACCCTTTGC CATGACCGGG GAAAAGGTGG ACCTGAATGA 7621 GGAGGAAACC ATTCTCATCA TCCGGCGTCT CCACAAAGTG CTGCGGCCCT TCTTGCTCCG 7681 ACGACTCAAG AAGGAAGTCG AGGCCCAGTT GCCCGAAAAG GTGGAGTACG TCATCAAGTG 7741 CGACATGTCT GCGCTGCAGC GAGTGCTCTA CCGCCACATG CAGGCCAAGG GCGTGCTGCT 7801 GACTGATGGC TCCGAGAAGG ACAAGAAGGG CAAAGGCGGC ACCAAGACCC TGATGAACAC 7861 CATCATGCAG CTGCGGAAGA TCTGCAACCA CCCCTACATG TTCCAGCACA TCGAGGAGTC 7921 CTTTTCCGAG CACTTGGGGT TCACTGGCGG CATTGTCCAA GGGCTGGACC TGTACCGAGC 7981 CTCGGGTAAA TTTGAGCTTC TTGATAGAAT TCTTCCCAAA CTCCGAGCAA CCAACCACAA 8041 AGTGCTGCTG TTCTGCCAAA TGACCTCCCT CATGACCATC ATGGAAGATT ACTTTGCGTA 8101 TCGCGGCTTT AAATACCTCA GGCTTGATGG AACCACGAAG GCGGAGGACC GGGGCATGCT 8161 GCTGAAAACC TTCAACGAGC CCGGCTCTGA GTACTTCATC TTCCTGCTCA GCACCCGGGC 8221 TGGGGGGCTC GGCCTGAACC TCCAGTCGGC AGACACTGTG ATCATTTTTG ACAGCGACTG 8281 GAATCCTCAC CAGGACCTGC AAGCGCAGGA CCGAGCCCAC CGCATCGGGC AGCAGAACGA 8341 GGTGCGTGTG CTCCGCCTCT GCACCGTCAA CAGCGTGGAG GAGAAGATCC TAGCTGCAGC 8401 CAAGTACAAG CTCAACGTGG ACCAGAAGGT GATCCAGGCC GGCATGTTCG ACCAGAAGTC 8461 CTCCAGCCAT GAGCGGCGCG CCTTCCTGCA GGCCATCCTG GAGCACGAGG AGCAGGATGA 8521 GAGCAGACAC TGCAGCACGG GCAGCGGCAG TGCCAGCTTC GCCCACACTG CCCCTCCGCC 8581 AGCGGGCGTC AACCCCGACT TGGAGGAGCC ACCTCTAAAG GAGGAAGACG AGGTGCCCGA 8641 CGACGAGACC GTCAACCAGA TGATCGCCCG GCACGAGGAG GAGTTTGATC TGTTCATGCG 8701 CATGGACCTG GACCGCAGGC GCGAGGAGGC CCGCAACCCC AAGCGGAAGC CGCGCCTCAT 8761 GGAGGAGGAC GAGCTCCCCT CGTGGATCAT CAAGGACGAC GCGGAGGTGG AGCGGCTGAC 8821 CTGTGAGGAG GAGGAGGAGA AGATGTTCGG CCGTGGCTCC CGCCACCGCA AGGAGGTGGA 8881 CTACAGCGAC TCACTGACGG AGAAGCAGTG GCTCAAGGCC ATCGAGGAGG GCACGCTGGA 8941 GGAGATCGAA GAGGAGGTCC GGCAGAAGAA ATCATCACGG AAGCGCAAGC GAGACAGCGA 9001 CGCCGGCTCC TCCACCCCGA CCACCAGCAC CCGCAGCCGC GACAAGGACG ACGAGAGCAA 9061 GAAGCAGAAG AAGCGCGGGC GGCCGCCTGC CGAGAAACTC TCCCCTAACC CACCCAACCT 9121 CACCAAGAAG ATGAAGAAGA TTGTGGATGC CGTGATCAAG TACAAGGACA GCAGCAGTGG 9181 ACGTCAGCTC AGCGAGGTCT TCATCCAGCT GCCCTCGCGA AAGGAGCTGC CCGAGTACTA 9241 CGAGCTCATC CGCAAGCCCG TGGACTTCAA GAAGATAAAG GAGCGCATTC GCAACCACAA 9301 GTACCGCAGC CTCAACGACC TAGAGAAGGA CGTCATGCTC CTGTGCCAGA ACGCACAGAC 9361 CTTCAACCTG GAGGGCTCCC TGATCTATGA AGACTCCATC GTCTTGCAGT CGGTCTTCAC 9421 CAGCGTGCGG CAGAAAATCG AGAAGGAGGA TGACAGTGAA GGCGAGGAGA GTGAGGAGGA 9481 GGAAGAGGGC GAGGAGGAAG GCTCCGAATC CGAATCTCGG TCCGTCAAAG TGAAGATCAA 9541 GCTTGGCCGG AAGGAGAAGG CACAGGACCG GCTGAAGGGC GGCCGGCGGC GGCCGAGCCG 9601 AGGGTCCCGA GCCAAGCCGG TCGTGAGTGA CGATGACAGT GAGGAGGAAC AAGAGGAGGA 9661 CCGCTCAGGA AGTGGCAGCG AAGAAGACga tatcacgcgt atgtgtacaG GCGGCGGCGG 9721 CAGCGGCGGC GGCGGCAGCG GCGGCGGCGG CAGCgctagc aaagacaata ctgtgcctct 9781 gaagctgatc gctctcctgg ctaatggcga gttccatagt ggcgaacagc tgggagaaac 9841 cctgggcatg tccagggccg ctatcaacaa gcacattcag actctgcgcg actggggcgt 9901 ggacgtgttc accgtgcccg gaaagggcta ctctctgccc gagcctatcc cgctgctgaa 9961 cgctaaacag attctgggac agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga 10021 ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg atgcttgcat 10081 tgcagaatat cagcaggcag ggagaggaag cagagggagg aaatggttct ctccttttgg 10141 agctaacctg tacctgagta tgttttggcg cctgaagcgg ggaccagcag caatcggcct 10201 gggcccggtc atcggaattg tcatggcaga agcgctgcga aagctgggag cagacaaggt 10261 gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag gcatcctggt 10321 ggagctggcc ggaataacag gcgatgctgc acagatcgtc attggcgccg ggattaacgt 10381 ggctatgagg cgcgtggagg aaagcgtggt caatcagggc tggatcacac tgcaggaagc 10441 agggattaac ctggacagga atactctggc cgctacgctg atccgagagc tgcgggcagc 10501 cctggaactg ttcgagcagg aaggcctggc tccatatctg ccacggtggg agaagctgga 10561 taacttcatc aatagacccg tgaagctgat cattggggac aaagagattt tcgggattag 10621 ccgggggatt gataaacagg gagccctgct gctggaacag gacggagtta tcaaaccctg 10681 gatgggcgga gaaatcagtc tgcggtctgc cgaaaagctg cagCCCAAGA AGAAGAGGAA 10741 GGTGGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA 10801 CGATGACAAG TAAgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 10861 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 10921 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 10981 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 11041 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 11101 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 11161 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 11221 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 11281 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 11341 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 11401 ctccccgcct gGAATTCTAC CGGGTAGGGG AGGCGCTTTT CCCAAGGCAG TCTGGAGCAT 11461 GCGCTTTAGC AGCCCCGCTG GGCACTTGGC GCTACACAAG TGGCCTCTGG CCTCGCACAC 11521 ATTCCACATC CACCGGTAGG CGCCAACCGG CTCCGTTCTT TGGTGGCCCC TTCGCGCCAC 11581 CTTCTACTCC TCCCCTAGTC AGGAAGTTCC CCCCCGCCCC GCAGCTCGCG TCGTGCAGGA 11641 CGTGACAAAT GGAAGTAGCA CGTCTCACTA GTCTCGTGCA GATGGACAGC ACCGCTGAGC 11701 AATGGAAGCG GGTAGGCCTT TGGGGCAGCG GCCAATAGCA GCTTTGCTCC TTCGCTTTCT 11761 GGGCTCAGAG GCTGGGAAGG GGTGGGTCCG GGGGCGGGCT CAGGGGCGGG CTCAGGGGCG 11821 GGGCGGGCGC CCGAAGGTCC TCCGGAGGCC CGGCATTCTG CACGCTTCAA AAGCGCACGT 11881 CTGCCGCGCT GTTCTCCTCT TCCTCATCTC CGGGCCTTTC GACCTGCAGC CCAAGCTTAC 11941 CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT 12001 ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA 12061 CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA 12121 CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA 12181 GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG 12241 TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA 12301 GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG 12361 CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT 12421 GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC 12481 CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG 12541 ACGCCCGCCC CACGACCCGC AGCGCCCGAC CGAAAGGAGC GCACGACCCC ATGCATCTCG 12601 AGGGCCCGGT ACctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 12661 taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt 12721 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctgcc 12781 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 12841 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 12901 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 12961 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 13021 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 13081 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc 13141 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 13201 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 13261 aggcttttgc gtcgagacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca 13321 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 13381 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 13441 ccttcccaac agttgcgcag cctgaatggc gaatggcgcg acgcgccctg tagcggcgca 13501 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 13561 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 13621 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 13681 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 13741 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 13801 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 13861 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 13921 ttaacgttta caatttcc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.