pLV3-CMV-SLFN12-3×FLAG-EGFP-EF

pLV3-CMV-SLFN12-3×FLAG-EGFP-EF1a-Puro plasmid

PVT106926

pLV3-CMV-SLFN12-3×FLAG-EGFP-EF1a-Puro plasmid Information

Plasmid name: pLV3-CMV-SLFN12(human)-3×FLAG-EGFP-EF1a-Puro
Alias: NM_018042.5;SLFN3
Gene lenght: 1734
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SLFN12-3×FLAG-EGFP-EF1a-Puro plasmid Description

pLV3-CMV-SLFN12-3×FLAG-EGFP-EF1a-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-SLFN12-3×FLAG-EGFP-EF1a-Puro plasmid Sequence

1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 181 tgggaggttt atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat 241 ccacgctgtt ttgacctcca tagaagattc tagagctagc gaattcgcca ccatgaacat 301 cagtgttgat ttggaaacga attatgccga gttggttcta gatgtgggaa gagtcactct 361 tggagagaac agtaggaaaa aaatgaagga ttgtaaactg agaaaaaagc agaatgaaag 421 tgtctcacga gctatgtgtg ctctgctcaa ttctggaggg ggagtgatca aggctgaaat 481 tgagaatgaa gactatagtt atacaaaaga tggaatagga ctagatttgg aaaattcttt 541 tagtaacatt ctgttatttg ttcctgagta cttagacttc atgcagaatg gtaactactt 601 tctgattttt gtgaagtcat ggagcttgaa cacctctggt ctgcggatta ccaccttgag 661 ctccaatttg tacaaaagag atataacatc tgcaaaagtc atgaatgcca ctgctgcact 721 ggagttcctc aaagacatga aaaagactag agggagattg tatttaagac cagaattgct 781 ggcaaagagg ccctgtgttg atatacaaga agaaaataac atgaaggcct tggccggggt 841 tttttttgat agaacagaac ttgatcggaa agaaaaattg acctttactg aatccacaca 901 tgttgaaatt aaaaacttct cgacagaaaa gttgttacaa cgaattaaag agattctccc 961 tcaatatgtt tctgcatttg caaatactga tggaggatat ttgttcattg gtttaaatga 1021 agataaagaa ataattggct ttaaagcaga gatgagtgac ctcgatgact tagaaagaga 1081 aatcgaaaag tccattagga agatgcctgt gcatcacttc tgtatggaga agaagaagat 1141 aaattattca tgcaaattcc ttggagtata tgataaagga agtctttgtg gatatgtctg 1201 tgcactcaga gtggagcgct tctgctgtgc agtgtttgct aaagagcctg attcctggca 1261 tgtgaaagat aaccgtgtga tgcagttgac caggaaggaa tggatccagt tcatggtgga 1321 ggctgaacca aaattttcca gttcatatga agaggtgatc tctcaaataa atacgtcatt 1381 acctgctccc cacagttggc ctcttttgga atggcaacgg cagagacatc actgtccagg 1441 gctatcagga aggataacgt atactccaga aaacctttgc agaaaactgt tcttacaaca 1501 tgaaggactt aagcaattaa tatgtgaaga aatggactct gtcagaaagg gctcactgat 1561 cttctctagg agctggtctg tggatctggg cttgcaagag aaccacaaag tcctctgtga 1621 tgctcttctg atttcccagg acagtcctcc agtcctatac accttccaca tggtacagga 1681 tgaggagttt aaaggctatt ctacacaaac tgccctaacc ttaaagcaga agctggcaaa 1741 aattggtggt tacactaaaa aagtgtgtgt catgacaaag atcttctact tgagccctga 1801 aggcatgaca agctgccagt atgatttaag gtcgcaagta atttaccctg aatcctacta 1861 ttttacaaga aggaaatact tgctgaaagc cctttttaaa gccttaaaga gactcaagtc 1921 tctgagagac cagttttcct ttgcagaaaa tctataccag ataatcggta tagattgctt 1981 tcagaagaat gataaaaaga tgtttaaatc ttgtcgaagg ctcaccCTCG AGGACTACAA 2041 AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGgg 2101 gcgcgccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 2161 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 2221 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 2281 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 2341 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 2401 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 2461 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 2521 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 2581 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 2641 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 2701 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 2761 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 2821 caagtaagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca 2881 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga 2941 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 3001 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 3061 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc 3121 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct 3181 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg 3241 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc 3301 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat 3361 ccaagctgtg accggcgcct acgctagatg accgagtaca agcccacggt gcgcctcgcc 3421 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 3481 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa 3541 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 3601 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 3661 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 3721 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 3781 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 3841 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 3901 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 3961 tgcatgaccc gcaagcccgg tgcctgagtc gacaatcaac ctctggatta caaaatttgt 4021 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 4081 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 4141 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 4201 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 4261 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 4321 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 4381 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc 4441 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 4501 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 4561 tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 4621 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 4681 aagataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 4741 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 4801 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 4861 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 4921 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 4981 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 5041 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 5101 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 5161 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 5221 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt 5281 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 5341 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 5401 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 5461 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 5521 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 5581 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 5641 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 5701 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 5761 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 5821 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 5881 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 5941 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 6001 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 6061 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 6121 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 6181 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 6241 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 6301 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 6361 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 6421 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 6481 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 6541 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 6601 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 6661 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 6721 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 6781 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 6841 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 6901 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 6961 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 7021 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 7081 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 7141 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 7201 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 7261 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 7321 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 7381 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 7441 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 7501 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 7561 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 7621 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 7681 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 7741 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 7801 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 7861 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctgacgcg 7921 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg 7981 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc 8041 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc 8101 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt 8161 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 8221 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 8281 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 8341 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc 8401 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac 8461 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat 8521 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa 8581 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga 8641 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc 8701 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat 8761 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 8821 gaccaccgca cagcaagcgg ccactgatct tcagacctgg aggaggagat atgagggaca 8881 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac 8941 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt 9001 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga 9061 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg 9121 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg 9181 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt 9241 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat 9301 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt 9361 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac 9421 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt 9481 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag 9541 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc 9601 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg 9661 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa 9721 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa 9781 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat 9841 cgatactagt attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc 9901 tacgtattag tcatcgctat taccatg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.