pLV3-CMV-HOXA10-EGFP-EF1a-Fluc

pLV3-CMV-HOXA10-EGFP-EF1a-Fluc-Neo plasmid

PVT110275

pLV3-CMV-HOXA10-EGFP-EF1a-Fluc-Neo plasmid Information

Plasmid name: pLV3-CMV-HOXA10(human)-EGFP-EF1a-Fluc-Neo
Alias: NM_018951.4;HOX1; HOX1.8; HOX1H; PL
Gene lenght: 1230
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green,Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HOXA10-EGFP-EF1a-Fluc-Neo plasmid Description

pLV3-CMV-HOXA10-EGFP-EF1a-Fluc-Neo It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-HOXA10-EGFP-EF1a-Fluc-Neo plasmid Sequence

1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa 61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac 121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa 181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc 241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc 421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc 481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt 541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga 601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat 661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt 721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag 781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa 841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa 901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg 961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg 1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac 1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag 1861 cagagatcca ctttggcgcc ggctcgagTC GCTATTACCA TGGTGATGCG GTTTTGGCAG 1921 TACATCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT 1981 GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC 2041 AACTCCGCCC CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT TTATATAAGC 2101 AGAGCTCGTT TAGTGAACCG TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC 2161 CATAGAAGAT TCTAGAGCCA CCatgtcagc cagaaagggc tatctgctcc cttcgccaaa 2221 ttatcccaca acaatgtcat gctcggagag ccccgccgcg aactcttttt tggtcgactc 2281 gctcatcagc tcgggcagag gcgaggcagg cggcggtggt ggtggcgcgg ggggcggcgg 2341 cggtggcggt tactacgccc acggcggggt ctacctgccg cccgccgccg acctgcccta 2401 cgggctgcag agctgcgggc tcttccccac gctgggcggc aagcgcaatg aggcagcgtc 2461 gccgggcagc ggtggcggtg gcgggggtct aggtcccggg gcgcacggct acgggccctc 2521 gcccatagac ctgtggctag acgcgccccg gtcttgccgg atggagccgc ctgacgggcc 2581 gccgccgccg ccccagcagc agccgccgcc cccgccgcaa ccaccccagc cagcgccgca 2641 ggccacctcg tgctctttcg cgcagaacat caaagaagag agctcctact gcctctacga 2701 ctcggcggac aaatgcccca aagtctcggc caccgccgcc gaactggctc ccttcccgcg 2761 gggcccgccg cccgacggct gcgccctggg cacctccagc ggggtgccag tgcctggcta 2821 cttccgcctt tctcaggcct acggcaccgc caagggctat ggcagcggcg gcggcggcgc 2881 gcagcaactc ggggctggcc cgttccccgc gcagcccccg gggcgcggtt tcgatctccc 2941 gcccgcgcta gcctccggct cggccgatgc ggcccggaag gagcgagccc tcgattcgcc 3001 gccgcccccc acgctggctt gcggcagcgg cgggggctcg cagggcgacg aggaggcgca 3061 cgcgtcgtcc tcggccgcgg aggagctctc cccggcccct tccgagagca gcaaagcctc 3121 gccggagaag gattccctgg gcaattccaa aggtgaaaac gcagccaact ggctcacggc 3181 aaagagtggt cggaagaagc gctgccccta cacgaagcac cagacactgg agctggagaa 3241 ggagtttctg ttcaatatgt accttactcg agagcggcgc ctagagatta gccgcagcgt 3301 ccacctcacg gacagacaag tgaaaatctg gtttcagaac cgcaggatga aactgaagaa 3361 aatgaatcga gaaaaccgga tccgggagct cacagccaac tttaattttt ccggtggagg 3421 cggatcagga ggcggtggat ctGAATTCGC CACCatggtg agcaagggcg aggagctgtt 3481 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 3541 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 3601 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt 3661 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 3721 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 3781 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 3841 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 3901 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 3961 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 4021 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 4081 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 4141 gatcactctc ggcatggacg agctgtacaa gtagtggctc cggtgcccgt cagtgggcag 4201 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg 4261 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt 4321 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc 4381 gcaacgggtt tgccgccaga acacaggtgt cgtgacgcgg gatccgccac catggaagac 4441 gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga 4501 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca 4561 gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg 4621 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa 4681 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg 4741 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct 4801 accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc 4861 ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg 4921 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca 4981 gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt 5041 ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga 5101 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc 5161 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc 5221 ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa 5281 agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa 5341 tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc 5401 ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact 5461 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt 5521 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc 5581 gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac 5641 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata 5701 gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag 5761 tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac 5821 atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc 5881 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc 5941 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg 6001 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag 6061 aagggcggaa agatcgccgt gggcagcggc gccaccaact tcagcctgct gaagcaggcc 6121 ggcgacgtgg aggagaaccc cggccccatg accgagtaca agcccatgat tgaacaagat 6181 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 6241 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 6301 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 6361 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 6421 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 6481 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 6541 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 6601 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 6661 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 6721 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 6781 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 6841 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 6901 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 6961 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 7021 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 7081 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 7141 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 7201 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 7261 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 7321 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc 7381 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 7441 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 7501 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 7561 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 7621 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg 7681 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 7741 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 7801 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 7861 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa 7921 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat 7981 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 8041 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac 8101 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 8161 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 8221 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat 8281 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc 8341 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc 8401 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt 8461 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 8521 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 8581 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 8641 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 8701 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 8761 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 8821 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 8881 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc 8941 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 9001 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 9061 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 9121 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 9181 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 9241 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 9301 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 9361 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 9421 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 9481 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 9541 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 9601 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 9661 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 9721 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 9781 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 9841 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 9901 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 9961 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 10021 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 10081 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 10141 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 10201 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 10261 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 10321 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 10381 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 10441 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 10501 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 10561 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 10621 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 10681 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 10741 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 10801 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 10861 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 10921 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 10981 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 11041 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac 11101 aaaagctgga gctgcaagc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.