pLV2-PRR15-3×FLAG-IRES-Puro pl

pLV2-PRR15-3×FLAG-IRES-Puro plasmid

PVT106619

pLV2-PRR15-3×FLAG-IRES-Puro plasmid Information

Plasmid name: pLV2-PRR15(human)-3×FLAG-IRES-Puro
Alias: NM_001329996.2
Gene lenght: 387
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-PRR15-3×FLAG-IRES-Puro plasmid Description

pLV2-PRR15-3×FLAG-IRES-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV2-PRR15-3×FLAG-IRES-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggcc 2821 gacagcggcg atgctggcag ctccggcccc tggtggaaat cgctcaccaa cagcagaaag 2881 aaaagcaagg aagccgcagt gggggtgccg cctcccgccc agcccgctcc cggggagccc 2941 acgccacctg cgccgcccag cccggactgg accagcagct cccgggagaa ccagcacccc 3001 aatctcctcg ggggcgccgg cgagcccccc aaaccagaca agttatacgg ggacaaatcc 3061 ggcagcagcc gccgcaattt gaagatctcg cgctccggcc gctttaagga gaagaggaaa 3121 gtgcgcgcca cgctgctccc ggaggcgggc aggtccccgg aggaggcagg ctttcctggt 3181 gacccccacg aggacaagca gctcgagGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 3241 CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGgatc ccgcccctct ccctcccccc 3301 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt 3361 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct 3421 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga 3481 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga 3541 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac 3601 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag 3661 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc 3721 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg 3781 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt 3841 gaaaaacacg atgataagct tgccacaacc cacaaggaga cgaccttcca tgaccgagta 3901 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc 3961 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga 4021 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 4081 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc 4141 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc 4201 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt 4261 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt 4321 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc 4381 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt 4441 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctaga cgcgtctgga 4501 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 4561 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 4621 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 4681 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 4741 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 4801 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 4861 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 4921 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 4981 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 5041 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta 5101 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac 5161 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac 5221 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 5281 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga 5341 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc 5401 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga 5461 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca 5521 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt 5581 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg 5641 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga 5701 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct 5761 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 5821 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 5881 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 5941 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 6001 ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg taatcatggt 6061 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 6121 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 6181 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 6241 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 6301 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 6361 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 6421 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 6481 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 6541 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 6601 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 6661 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 6721 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 6781 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 6841 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 6901 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 6961 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 7021 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 7081 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 7141 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 7201 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 7261 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 7321 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 7381 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 7441 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 7501 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 7561 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 7621 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 7681 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 7741 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 7801 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 7861 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 7921 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 7981 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 8041 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 8101 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 8161 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 8221 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 8281 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 8341 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc 8401 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa 8461 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact 8521 acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag 8581 t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.