pIRF3-EGFP plasmid

pIRF3-EGFP plasmid

PVT106596

pIRF3-EGFP plasmid Information

Plasmid name: pIRF3-EGFP
Alias:
Gene lenght:
Host: Mammalian cells
Use(s): Transcription factor reports
Fragment type: Element
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: IRF3
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pIRF3-EGFP plasmid Description

pIRF3-EGFP is a plasmid suitable for transcription factor reporting in mammalian cells.

pIRF3-EGFP plasmid Sequence

1 TCGAAGCTTG AAAACTGAAA GAGAGAAGTG AAAGTGGGGA AAACTGAAAG GGAGAAGTGA 61 AAGTGGGGAA AACTGAAAGG GAGAAGTGAA AGTGGGGAAA ACTGAAAGGG AGAAGTGAAA 121 GTGGTCGACT CTAGAGGATC CGGTTCGCAT ATTAAGGTGA CGCGTGTGGC CTCGAACACC 181 GAGCGACCCT GCAGCGACCC GCTTAACAGC GTCAACAGCG TGCCGCAGAT CTCGAGATCT 241 GAGCTTGGCA TTCCGGTACT GTTGGTAAAg ccaccatggt gagcaagggc gaggagctgt 301 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 361 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 421 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 481 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 541 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 601 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 661 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 721 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 781 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 841 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 901 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 961 ggatcactct cggcatggac gagctgtaca agtagtctag agtcggggcg gccggccgct 1021 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 1081 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 1141 ctgcaataaa caagttAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA 1201 GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGATCCCCG 1261 GGTACCGAGC TCGAATTCGT AATCATGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG 1321 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA 1381 TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC 1441 CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT 1501 GGgcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 1561 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 1621 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 1681 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 1741 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 1801 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 1861 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 1921 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 1981 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 2041 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 2101 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 2161 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 2221 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 2281 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 2341 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 2401 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 2461 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 2521 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 2581 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 2641 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 2701 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 2761 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 2821 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 2881 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 2941 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 3001 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 3061 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 3121 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 3181 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 3241 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 3301 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 3361 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 3421 ccccgaaaag tgccacctgA ACTTGTTTAT TGCAGCTTAT AATGGTTACC AAATAAAGCA 3481 ATAGCATCCC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 3541 CCAAACTCAT CAATGTATCT TATCATGTCT GGATCAGCCA TATCACATTT GTAGAGGTTT 3601 TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA 3661 TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 3721 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 3781 ATGTATCTTA TCATGTCTGG ATC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.