pLV3-CMV-Xpo1-3×HA-CopGFP-Puro

pLV3-CMV-Xpo1-3×HA-CopGFP-Puro plasmid

PVT110025

pLV3-CMV-Xpo1-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Xpo1(mouse)-3×HA-CopGFP-Puro
Alias: NM_001412408.1;Crm1; Exp1
Gene lenght: 3213
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Xpo1-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-Xpo1-3×HA-CopGFP-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-Xpo1-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCATGC CAGCAATTAT GACAATGTTA 2341 GCAGACCATG CAGCTCGTCA GCTGCTTGAT TTCAGCCAAA AACTGGATAT CAACCTTTTA 2401 GACAACGTGG TGAATTGCTT ATACCATGGA GAAGGAGCCC AGCAAAGAAT GGCTCAAGAA 2461 GTACTGACAC ATTTAAAGGA GCATCCTGAT GCTTGGACAC GGGTCGACAC AATATTGGAG 2521 TTTTCGCAGA ACATGAACAC GAAATACTAT GGACTACAGA TTTTGGAAAA TGTGATCAAA 2581 ACAAGGTGGA AGATCCTTCC AAGGAACCAG TGTGAAGGAA TAAAAAAATA TGTTGTTGGT 2641 CTCATTATCA AGACATCCTC TGACCCAACT TGTGTAGAGA AAGAAAAGGT GTATATTGGG 2701 AAATTGAATA TGATTCTTGT TCAGATACTT AAGCAAGAGT GGCCTAAGCA TTGGCCAACT 2761 TTTATCAGTG ACATTGTTGG AGCGAGTAGG ACCAGTGAAA GTCTATGTCA AAATAATATG 2821 GTGATTCTTA AACTCTTGAG TGAAGAAGTG TTTGATTTCT CTAGTGGACA GATAACCCAA 2881 GTTAAAGCAA AGCATTTAAA AGACAGCATG TGCAATGAGT TTTCACAAAT ATTTCAGCTT 2941 TGTCAGTTTG TGATGGAAAA TTCCCAAAAT GCTCCCCTTG TTCATGCTAC ATTAGAAACA 3001 CTGCTGAGAT TTCTGAACTG GATTCCACTG GGATACATCT TTGAGACCAA GTTAATCAGC 3061 ACATTAATTT ACAAGTTCCT GAATGTTCCA ATGTTCCGAA ATGTGTCTCT AAAGTGCCTC 3121 ACTGAGATTG CTGGTGTGAG TGTAAGCCAA TATGAGGAAC AATTTGAAAC TCTGTTTACA 3181 CTTACAATGA TGCAGCTAAA ACAGATGCTT CCTTTAAACA CTAATATCCG ACTTGCATAC 3241 TCGAATGGGA AAGATGATGA ACAGAACTTT ATTCAAAATC TCAGCCTGTT TCTCTGTACC 3301 TTTCTAAAAG AGCACGGTCA ACTTCTAGAA AAACGACTGA ATCTCAGGGA AGCACTCATG 3361 GAGGCTCTTC ATTATATGTT GTTGGTGTCT GAAGTGGAAG AAACTGAAAT TTTTAAAATC 3421 TGCCTTGAGT ACTGGAATCA TTTGGCAGCT GAGCTCTACA GAGAGAGTCC ATTTTCTACA 3481 TCTGCTTCTC CCTTGTTATC TGGAAGTCAA CACTTTGATA TTCCTCCTAG GAGACAGCTG 3541 TACTTGACCG TGTTATCAAA GGTCCGTTTA TTGATGGTTA GTCGCATGGC TAAACCAGAG 3601 GAGGTACTGG TTGTAGAAAA TGATCAGGGA GAAGTTGTAA GAGAGTTCAT GAAGGATACT 3661 GATTCCATAA ATTTATATAA GAATATGAGA GAAACATTAG TTTATCTTAC TCATCTGGAT 3721 TATGTAGATA CGGAAATAAT AATGACTAAG AAACTTCAAA ATCAAGTGAA TGGTACAGAA 3781 TGGTCATGGA AGAATTTGAA TACGTTGTGT TGGGCAATAG GCTCCATCAG TGGCGCAATG 3841 CATGAAGAGG ATGAGAAGCG GTTTCTTGTT ACTGTTATAA AGGATCTTTT AGGATTATGT 3901 GAGCAGAAAA GAGGCAAAGA TAATAAAGCT ATTATTGCAT CGAATATCAT GTACATAGTA 3961 GGTCAATACC CAAGATTTTT GAGAGCACAC TGGAAATTTT TGAAGACAGT AGTAAACAAG 4021 CTATTCGAAT TCATGCATGA GACCCATGAT GGAGTCCAGG ATATGGCTTG TGACACATTT 4081 ATTAAAATAG CTCAAAAATG CCGTAGGCAT TTTGTTCAGG TTCAAGTTGG AGAAGTAATG 4141 CCGTTCATTG ATGAGATTTT AAACAACATA AATACTATAA TTTGTGATCT TCAGCCTCAG 4201 CAGGTTCATA CATTTTATGA AGCTGTGGGG TACATGATTG GTGCACAGAC AGACCAGACA 4261 GTTCAAGAGC ATTTGATAGA AAAATACATG CTACTCCCTA ATCAAGTGTG GGACAGTATA 4321 ATTCAGCAGG CTACCAAAAA TGTGGATATC CTGAAAGATC CTGAAACAGT CAAGCAGCTG 4381 GGTAGCATAT TAAAAACAAA TGTGAGAGCC TGCAAAGCTG TTGGTCATCC CTTTGTAATT 4441 CAGCTTGGCA GAATTTATTT AGACATGCTT AATGTATATA AGTGCCTCAG TGAAAATATT 4501 TCTGCAGCTA TTCAAGCTAA TGGTGAGATG GTTACAAAGC AACCATTGAT TAGAAGTATG 4561 CGGACAGTAA AAAGGGAAAC TTTAAAGTTA ATATCTGGTT GGGTGAGCCG TTCCAATGAT 4621 CCTCAGATGG TAGCTGAAAA CTTTGTTCCT CCTTTGTTGG ATGCAGTTCT TATTGATTAC 4681 CAGAGAAATG TACCAGCTGC TAGAGAACCA GAAGTGCTTA GTACTATGGC CATTATTGTC 4741 AACAAATTAG GTGGACATAT AACAGCTGAA ATACCTCAAA TATTCGATGC AGTTTTTGAG 4801 TGCACATTGA ATATGATAAA TAAGGATTTT GAAGAGTATC CAGAACACAG AACAAACTTT 4861 TTCTTATTAC TTCAAGCTGT CAATTCTCAC TGTTTCCCAG CATTCCTAGC TATTCCACCT 4921 GCACAGTTCA AGCTGGTTTT GGATTCTATT ATTTGGGCTT TTAAACATAC TATGAGGAAT 4981 GTTGCAGATA CAGGCTTACA GATACTTTTT ACACTTTTAC AAAATGTTGC ACAAGAGGAA 5041 GCTGCCGCAC AGAGTTTTTA TCAAACTTAT TTTTGTGATA TTCTTCAGCA TATTTTCTCT 5101 GTTGTAACAG ACACTTCACA TACTGCAGGT CTAACAATGC ATGCATCAAT TCTTGCATAT 5161 ATGTTTAATT TGGTTGAAGA AGGAAAAATA AGTACACCAC TAAATCCTGG AAATCCAGTT 5221 AACAACCAAA TGTTTATTCA AGACTATGTG GCTAATCTCC TTAAATCTGC ATTCCCTCAT 5281 CTACAAGATG CTCAAGTAAA GCTCTTTGTA ACAGGACTTT TCAGCTTAAA TCAGGATATT 5341 CCTGCTTTCA AAGAACACCT AAGGGATTTC TTAGTTCAAA TAAAGGAGTT TGCAGGTGAA 5401 GATACATCGG ATCTATTTTT GGAAGAAAGA GAAACAGCCC TTCGACAGGC TCAGGAGGAG 5461 AAACATAAAC TTCAAATGTC TGTACCTGGG ATTCTTAATC CACACGAAAT TCCAGAAGAA 5521 ATGTGTGATC TCGAGTACCC ATACGACGTA CCTGATTATG CAACTTATCC TTATGATGTA 5581 CCTGATTATG CTAGCTATCC TTATGATGTC CCAGACTACG CATAGggatc cCTGCGATAT 5641 CCAGCgcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca 5701 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga 5761 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 5821 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 5881 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc 5941 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt 6001 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc 6061 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg 6121 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc 6181 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc 6241 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg 6301 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa 6361 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac 6421 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc 6481 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc 6541 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc 6601 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg 6661 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac 6721 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc 6781 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc 6841 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc 6901 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc 6961 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc 7021 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 7081 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 7141 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 7201 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 7261 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 7321 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 7381 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 7441 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 7501 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 7561 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 7621 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 7681 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 7741 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 7801 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 7861 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 7921 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 7981 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg 8041 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 8101 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 8161 ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg cctggtacct 8221 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 8281 ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg 8341 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 8401 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 8461 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 8521 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 8581 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 8641 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 8701 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc 8761 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 8821 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag 8881 acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 8941 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 9001 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 9061 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 9121 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 9181 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 9241 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 9301 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 9361 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 9421 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 9481 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 9541 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 9601 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 9661 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 9721 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 9781 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 9841 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 9901 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 9961 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 10021 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 10081 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 10141 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 10201 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 10261 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 10321 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 10381 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 10441 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 10501 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 10561 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 10621 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 10681 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 10741 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 10801 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 10861 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 10921 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 10981 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 11041 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 11101 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac 11161 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 11221 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat 11281 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 11341 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 11401 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 11461 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 11521 ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.