pLV3-CMV-Met-3×FLAG-Puro plasm

pLV3-CMV-Met-3×FLAG-Puro plasmid

PVT106484

pLV3-CMV-Met-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-Met(mouse)-3×FLAG-Puro
Alias: NM_008591.3;c-Met; HGF; HGFR; Par4
Gene lenght: 4137
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Met-3×FLAG-Puro plasmid Description

pLV3-CMV-Met-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-Met-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgaaggct cccaccgtgc tggcacctgg cattctggtg 2341 ctgctgttgt ccttggtgca gaggagccat ggggagtgca aggaggccct agtgaagtct 2401 gagatgaacg tgaacatgaa gtatcagctc cccaacttca cggcagaaac ccccatccag 2461 aatgtcgtcc tacacggcca tcatatttat ctcggagcca caaactacat ttatgtttta 2521 aatgacaaag accttcagaa ggtatccgaa ttcaagaccg ggcccgtgtt ggaacaccca 2581 gattgtttac cttgtcggga ctgcagcagc aaagccaatt catcaggagg ggtttggaaa 2641 gacaacatca acatggctct gcttgttgac acatactatg atgatcaact cattagctgt 2701 ggcagtgtca acagagggac ttgccagcgg catgtccttc ctcctgacaa ttctgctgac 2761 atccagtctg aggtccactg catgttctcc ccagaagagg agtcagggca gtgtcctgac 2821 tgtgtagtga gtgccctcgg agccaaagtc ctcctgtcgg aaaaggaccg gttcatcaat 2881 ttctttgtgg ggaatacgat caattcctcc tatcctcctg gttattcact gcattcgata 2941 tcggtgagac ggctgaagga aacccaagat ggttttaagt ttttgacaga ccagtcctat 3001 attgatgtct taccagaatt ccaagattcc taccccataa agtacataca tgccttcgaa 3061 agcaaccatt ttatttactt tctgactgtc caaaaggaaa ctctagatgc tcagactttt 3121 catacaagaa taatcaggtt ctgttccgta gactctgggt tgcactccta catggaaatg 3181 cccctggaat gcatcctgac agaaaaaaga aggaagagat ccacaaggga agaagtgttt 3241 aatatcctcc aagccgcgta tgtcagtaaa ccaggggcca atcttgctaa gcaaatagga 3301 gctagccctt ctgatgacat tctcttcggg gtgtttgcac aaagcaagcc agattctgct 3361 gaacctgtga atcgatcagc agtctgtgca ttccccatca aatatgtcaa tgacttcttc 3421 aacaagattg tcaacaaaaa caacgtgaga tgtctccagc atttttacgg acccaaccat 3481 gagcactgtt tcaataggac cctgctgaga aactcttccg gctgtgaagc gcgcagtgac 3541 gagtatcgga cagagtttac cacggctttg cagcgcgtcg acttattcat gggccggctt 3601 aaccaagtgc tcctgacatc catctccacc ttcatcaaag gtgacctcac cattgctaat 3661 ctagggacgt cagaaggtcg cttcatgcag gtggtgctct ctcgaacagc acacctcact 3721 cctcatgtga acttcctcct ggactcccat cctgtatctc cagaagttat tgttgagcat 3781 ccatcaaatc aaaatggcta tacattggtt gtcacaggaa agaagatcac caagattcca 3841 ttgaatggcc tgggctgtgg acatttccaa tcctgcagtc agtgcctctc tgccccttac 3901 tttatacagt gtggctggtg ccacaatcaa tgtgtgcgtt ttgatgaatg ccccagcggt 3961 acatggactc aagagatctg tctgccggcg gtttataagg tgttccccac cagcgcgccc 4021 cttgaaggag gaacagtgtt gaccatatgt ggctgggact ttggattcag gaagaataat 4081 aaatttgatt taaggaaaac caaagttctg cttggcaacg agagctgtac cttgacctta 4141 agcgagagca cgacaaatac gttgaaatgc acagttggtc ccgcgatgag tgagcacttc 4201 aatgtgtctg taattatctc aaacagtcga gagacgacgc aatacagtgc attctcctat 4261 gtagatcctg taataacaag catttctccg aggtacggcc ctcaggctgg aggcacctta 4321 ctcactctta ctgggaaata cctcaacagt ggcaattcta gacacatttc aattggaggg 4381 aaaacatgta ctttaaaaag tgtatcagat agtattcttg aatgctacac cccagcccaa 4441 actacctctg atgagtttcc tgtgaaattg aagattgact tggctaaccg agagaccagc 4501 agcttcagtt accgggaaga ccccgttgtc tatgaaatcc acccgaccaa atcttttatt 4561 agtggtggaa gcacaataac gggtattggg aagaccctga actcggttag cctcccaaag 4621 ctggtaatag atgtgcatga agtgggtgtg aactacacag tggcatgtca gcatcgctca 4681 aattcagaga tcatctgctg cactactcct tcactgaaac agctgggcct gcaactcccc 4741 ctgaagacca aagccttctt cctgttagac gggattcttt ccaaacactt tgatctcact 4801 tatgtgcata atcctgtgtt tgagcctttt gaaaagccag taatgatctc aataggcaat 4861 gaaaatgtag tggaaattaa gggaaacaat attgaccctg aagcagttaa aggtgaagtg 4921 ttaaaagttg gaaatcagag ctgcgagagt ctccactggc actctggagc tgtgttgtgt 4981 acagtcccca gtgacctgct caaactgaac agcgagctaa atatagagtg gaagcaagca 5041 gtctcttcaa ctgttcttgg aaaagtgatc gttcaaccgg atcagaattt tgcaggattg 5101 atcattggtg cggtctcaat atcagtagta gttttgttat tatccgggct cttcctgtgg 5161 atgagaaaga gaaagcataa agatctgggc agtgaattag ttcgctatga cgcaagagta 5221 cacactcctc atttggatag gcttgtaagt gcccgaagtg taagtccaac tacagagatg 5281 gtttcaaatg agtctgtaga ctacagagct acttttccag aagaccagtt tcccaactcc 5341 tctcagaatg gagcatgcag acaagtgcaa taccctctga cagacctgtc ccctatcctg 5401 acaagtggag actctgatat atccagccca ttactacaaa atactgttca cattgacctc 5461 agtgctctaa atccagagct ggtccaagca gttcagcacg tagtgattgg acccagcagc 5521 ctgattgtgc atttcaatga agtcatagga agagggcatt ttggctgtgt ctatcatggg 5581 actttgctgg acaatgacgg aaagaaaatt cactgtgctg tgaaatcctt aaatagaatc 5641 acagatatag aagaggtctc ccagtttctg actgagggaa tcatcatgaa agacttcagc 5701 catcccaatg ttctctcact cttgggaatc tgcctgagga gtgaagggtc tcctctggtg 5761 gtcctgccct atatgaagca tggagatctg cgaaatttca ttcgaaacga gactcataat 5821 ccaactgtga aagatcttat aggatttggc cttcaagtag ccaaaggcat gaaatatctt 5881 gccagcaaaa agtttgtcca cagagactta gctgcaagaa actgcatgtt ggatgaaaaa 5941 ttcactgtca aggttgctga tttcggtctt gccagagaca tgtacgataa agagtactat 6001 agtgtccaca acaagacggg tgccaagcta ccagtaaagt ggatggcttt agagagtctg 6061 caaacgcaga agttcaccac caagtcagat gtgtggtcct ttggtgtgct cctctgggag 6121 ctcatgacga gaggagcccc tccttatccc gacgtgaaca catttgatat cactatctac 6181 ctgttgcaag gcagaagact cttgcaacca gaatactgtc cagacgcctt gtacgaagtg 6241 atgctaaaat gctggcaccc caaagcggaa atgcgcccgt ccttttccga actggtctcc 6301 aggatatcct caatcttctc cacgttcatt ggggaacact acgtccacgt gaacgctact 6361 tatgtgaatg taaaatgtgt tgctccatat ccttctctgt tgccatccca agacaacatt 6421 gatggcgagg ggaacacaGG ATCCGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 6481 GACATCGACT ACAAGGATGA CGATGACAAG TAGCGGCCGC GAAGGATCTG CGATCGCTCC 6541 GGTGCCCGTC AGTGGGCAGA GCGCACATCG CCCACAGTCC CCGAGAAGTT GGGGGGAGGG 6601 GTCGGCAATT GAACGGGTGC CTAGAGAAGG TGGCGCGGGG TAAACTGGGA AAGTGATGTC 6661 GTGTACTGGC TCCGCCTTTT TCCCGAGGGT GGGGGAGAAC CGTATATAAG TGCAGTAGTC 6721 GCCGTGAACG TTCTTTTTCG CAACGGGTTT GCCGCCAGAA CACAGCTGAA GCTTCGAGGG 6781 GCTCGCATCT CTCCTTCACG CGCCCGCCGC CCTACCTGAG GCCGCCATCC ACGCCGGTTG 6841 AGTCGCGTTC TGCCGCCTCC CGCCTGTGGT GCCTCCTGAA CTGCGTCCGC CGTCTAGGTA 6901 AGTTTAAAGC TCAGGTCGAG ACCGGGCCTT TGTCCGGCGC TCCCTTGGAG CCTACCTAGA 6961 CTCAGCCGGC TCTCCACGCT TTGCCTGACC CTGCTTGCTC AACTCTACGT CTTTGTTTCG 7021 TTTTCTGTTC TGCGCCGTTA CAGATCCAAG CTGTGACCGG CGCCTACGCT AGATGACCGA 7081 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCCGGGCCG TACGCACCCT 7141 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGACCCGG ACCGCCACAT 7201 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA 7261 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA 7321 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT 7381 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG 7441 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT 7501 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC 7561 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA 7621 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GAGTCGACAA 7681 TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC 7741 TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT 7801 GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG 7861 GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG 7921 TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT 7981 TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT 8041 GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC 8101 CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA 8161 TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG 8221 CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GTACCTTTAA 8281 GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC 8341 TGGAAGGGCT AATTCACTCC CAACGAAAAT AAGATCTGCT TTTTGCTTGT ACTGGGTCTC 8401 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 8461 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 8521 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTAGTA 8581 GTTCATGTCA TCTTATTATT CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG 8641 AGAGGAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 8701 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 8761 TATCTTATCA TGTCTGGCTC TAGCTATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC 8821 TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC 8881 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGACTTTT GCAGAGACGG 8941 CCCAAATTCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT 9001 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG 9061 CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG 9121 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC 9181 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC 9241 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 9301 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 9361 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 9421 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 9481 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 9541 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 9601 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 9661 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 9721 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 9781 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 9841 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 9901 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 9961 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 10021 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 10081 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA 10141 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT 10201 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG 10261 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA 10321 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA 10381 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG 10441 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC 10501 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC 10561 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA 10621 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC 10681 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG 10741 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC 10801 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG 10861 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC 10921 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT 10981 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 11041 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 11101 AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG 11161 TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT 11221 GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 11281 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA 11341 GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG 11401 AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC 11461 GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT 11521 AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA 11581 GCTG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.