pBiT2.1-KDM5A-TK-SmBiT plasmid

pBiT2.1-KDM5A-TK-SmBiT plasmid

PVT106434

pBiT2.1-KDM5A-TK-SmBiT plasmid Information

Plasmid name: pBiT2.1-KDM5A(human)-TK-SmBiT
Alias: NM_001042603.3;RBBP-2; RBBP2; RBP2
Gene lenght: 5070
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: TK
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: HSV TK-F1
Reverse primer:
Copy number: High
Induction:

pBiT2.1-KDM5A-TK-SmBiT plasmid Description

pBiT2.1-KDM5A-TK-SmBiT is a plasmid suitable for protein expression in mammalian cells.

pBiT2.1-KDM5A-TK-SmBiT plasmid Sequence

1 GGCCTAACTG GCCGGTACCT GAGTCTAAAT GAGTCTTCGG ACCTCGCGGG GGCCGCTTAA 61 GCGGTGGTTA GGGTTTGTCT GACGCGGGGG GAGGGGGAAG GAACGAAACA CTCTCATTCG 121 GAGGCGGCTC GGGGTTTGGT CTTGGTGGCC ACGGGCACGC AGAAGAGCGC CGCGATCCTC 181 TTAAGCACCC CCCCGCCCTC CGTGGAGGCG GGGGTTTGGT CGGCGGGTGG TAACTGGCGG 241 GCCGCTGACT CGGGCGGGTC GCGCGCCCCA GAGTGTGACC TTTTCGGTCT GCTCGCAGAC 301 CCCCGGGCGG CGCCGCCGCG GCGGCGACGG GCTCGCTGGG TCCTAGGCTC CATGGGGACC 361 GTATACGTGG ACAGGCTCTG GAGCATCCGC ACGACTGCGG TGATATTACC GGAGACCTTC 421 TGCGGGACGA GCCGGGTCAC GCGGCTGACG CGGAGCGTCC GTTGGGCGAC AAACACCAGG 481 ACGGGGCACA GGTACACTAT CTTGTCACCC GGAGGCGCGA GGGACTGCAG GAGCTTCAGG 541 GAGTGGCGCA GCTGCTTCAT CCCCGTGGCC CGTTGCTCGC GTTTGCTGGC GGTGTCCCCG 601 GAAGAAATAT ATTTGCATGT CTTTAGTTCT ATGATGACAC AAACCCCGCC CAGCGTCTTG 661 TCATTGGCGA AGTCGAACAC GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG 721 CATATTAAGG TGACGCGTGT GGCCTCGAAC ACCGAGCGAC CCTGCAGCGA CCCGCTTAAA 781 AGCTTGGCAA TCCGGTACTG TGGTAAAGCC ACCAGATCTG CTAGCGATCG CCTAAGTGGG 841 AGCTCAGGGG AATTCcgcca ccatggcggg cgtggggccg gggggctacg cggcggagtt 901 cgtgccaccg ccagagtgcc ccgtctttga gccgagttgg gaggagttca cagatccgct 961 cagctttatc ggccgcatcc ggcctttggc ggagaaaacc ggcatctgca aaattcggcc 1021 gcccaaggac tggcagcctc catttgcctg tgaagtaaaa agctttcgtt tcactccaag 1081 agtccagcgc ctgaatgaac ttgaggcaat gaccagagtg agattggatt tcttggatca 1141 actagcaaaa ttttgggaac ttcaaggatc tactctgaag atccctgtgg tagagagaaa 1201 aatcctggat ctgtatgctt tgagcaagat tgttgccagc aaaggaggtt ttgaaatggt 1261 caccaaagag aagaaatggt ctaaagtggg tagtcgcttg ggatatctgc caggaaaagg 1321 aactgggtct cttttgaagt cacattatga aagaattctc tacccatatg agcttttcca 1381 gtctggtgtg agccttatgg gtgtgcagat gcctaattta gatcttaaag aaaaagtgga 1441 gcctgaggtt ctcagcactg atacccaaac ttccccagag ccaggcacaa ggatgaacat 1501 tctgccgaag agaacaagac gtgtgaagac tcagtcagaa tctggagatg tgagtagaaa 1561 cacggaactg aagaaacttc agatttttgg ggctgggccc aaggttgtgg gcttggcaat 1621 gggaacaaaa gataaagaag atgaggtcac ccgaagacga aaagttacca acaggtcaga 1681 cgcatttaac atgcaaatga gacaacggaa aggcactctc tctgttaact ttgttgatct 1741 ctatgtttgt atgttttgtg gtcggggaaa caatgaagat aaattgcttt tgtgtgatgg 1801 atgtgatgac agctatcata cattttgtct aattcctcca ctacctgatg tgcccaaagg 1861 agactggagg tgtcctaaat gtgtcgccga ggaatgtagc aaacctcgag aagcctttgg 1921 atttgaacaa gctgtacgag agtatacact tcagagcttt ggagagatgg cagataattt 1981 taagtctgat tattttaata tgccagtcca tatggttccc acagaactag tagaaaagga 2041 attttggcgg ctggtaagca gcattgaaga agatgttatt gtggaatatg gagcagatat 2101 ctcctcaaaa gactttggaa gtggatttcc ggtgaaggat gggcggagaa agattctgcc 2161 agaagaagag gaatatgcac tttctggttg gaatttgaat aacatgcctg tcctggaaca 2221 gtctgttctt gcacatatta atgtggacat ctctggtatg aaagtgccgt ggctctatgt 2281 gggaatgtgc ttctcttctt tttgctggca cattgaggat cactggagtt attccatcaa 2341 ctacttgcac tggggggagc caaagacatg gtatggtgtg ccatctcatg ctgcagagca 2401 actggaggag gtgatgagag agctggcccc cgagttattt gaatcccagc ctgatcttct 2461 gcatcagtta gttaccatca tgaaccccaa cgtgctaatg gagcatggtg tgcctgtgta 2521 caggaccaat cagtgtgctg gcgagtttgt tgtgacattt cctcgtgcct atcactctgg 2581 atttaaccag ggctacaact ttgctgaagc tgtgaacttc tgtactgctg actggttgcc 2641 cattggacgt caatgtgtaa atcattaccg acgcctaagg cgccactgtg tcttttcaca 2701 cgaggaacta attttcaaga tggcagcaga tccagaatgc ttagatgtgg ggctggctgc 2761 catggtctgc aaagaattga ctctcatgac tgaagaagaa acacgattaa gagagtctgt 2821 tgtacagatg ggtgtcctga tgtcagaaga agaagtgttt gaacttgttc ctgatgatga 2881 gcggcagtgt tcagcatgca gaaccacatg ttttctctct gctctcacat gttcctgtaa 2941 tcctgagcgg cttgtatgtc tctaccatcc aactgatctg tgcccctgcc ccatgcagaa 3001 gaaatgtctt agatatcgct acccattaga agacctccct tctctgctat atggtgtaaa 3061 agtcagggca cagtcctatg acacttgggt cagtcgtgtt acagaagcat tgtctgctaa 3121 cttcaaccac aaaaaagatt tgattgaatt gcgagtaatg ctggaagatg ctgaggatag 3181 gaaataccca gagaatgatc tctttcgaaa actcagggat gctgtaaaag aagctgagac 3241 ctgtgcttct gtggctcagc tgcttctgag caaaaagcag aaacacagac agagcccaga 3301 tagtgggagg actcggacca aactgacagt ggaagaattg aaggcctttg tccaacaact 3361 ttttagtctt ccgtgtgtca tcagccaagc tcggcaagta aagaatctgc tagatgatgt 3421 ggaagagttt catgaacgtg ctcaggaggc catgatggat gaaaccccag attcttccaa 3481 actccagatg ttgatagata tgggctctag tctctatgtg gaactccctg aattaccacg 3541 actgaagcaa gagctacaac aggctcggtg gttggacgaa gtaagactga ccttatcaga 3601 tccgcaacaa gtcactttgg atgtcatgaa gaagctgata gactctgggg tagggttggc 3661 accccaccat gctgtggaga aagcaatggc tgaactacag gagctcctta cagtctctga 3721 acgatgggaa gaaaaggcta aggtctgcct acaggcaaga ccgaggcaca gtgtggcaag 3781 tttagaaagc attgtgaatg aagccaagaa cattccagcc tttctaccca atgtgttgtc 3841 cttgaaagaa gccttacaaa aggctcgaga atggaccgct aaagtggaag ctattcagag 3901 tggcagcaat tacgcttatt tggagcagct tgagagcttg tctgcgaaag gacgccctat 3961 tcctgtgcgt cttgaagcac tgccgcaagt ggaatcacag gtagcagcag cacgggcatg 4021 gagagaacgg actgggcgga cgtttcttaa gaagaattct agccatacat tgttacaggt 4081 gctgagcccc cggaccgaca ttggtgtata tgggagtggc aaaaatagga ggaaaaaagt 4141 aaaagaacta atagaaaaag aaaaagaaaa ggatctggac ctggagcctc tgagtgatct 4201 ggaggaagga ttggaggaaa ccagagatac agccatggtg gtggcagttt tcaaagaacg 4261 ggagcaaaaa gagattgaag ccatgcattc tctcagagca gccaacctag ccaagatgac 4321 aatggtggac cgcatagaag aagtaaaatt ttgcatttgc cgcaagacag ccagtgggtt 4381 tatgctacag tgtgagctct gcaaagactg gttccataac agctgtgttc ctcttcctaa 4441 atcaagttcc caaaaaaaag gatccagctg gcaagctaaa gaagtaaaat tcctttgccc 4501 tctttgtatg cggtctcgaa ggcccaggct agagactatt ctgtcactcc tggtatccct 4561 tcagaagttg cccgtacggt tgcctgaagg agaggccctg cagtgtttga cagaacgtgc 4621 tatgagttgg caagatagag cgcggcaggc tctagccaca gatgaactat cctctgccct 4681 ggccaaacta tctgtgttga gccagcgtat ggtggaacag gcggctcgag aaaaaactga 4741 aaagatcatc agtgcagaac tccaaaaagc agctgccaat ccagacttac agggacactt 4801 acctagtttc cagcagtctg cttttaaccg ggtggtgagc agtgtgtcat cttctcctcg 4861 acaaacaatg gactatgatg atgaagaaac agactctgat gaagacattc gagagacata 4921 tggctacgac atgaaggaca cagccagtgt gaagtcctct agtagtcttg aacccaatct 4981 tttttgtgat gaagagattc ccatcaaatc cgaggaggtg gtgacccaca tgtggacagc 5041 accttcattt tgtgcagagc atgcttattc ttctgcttct aagagttgtt ctcaaggttc 5101 tagcacccca aggaaacaac ctcggaagag ccctttggtg ccccgaagtt tggaacctcc 5161 agtgctggag ttgtcacctg gagctaaggc acaactggaa gaacttatga tggttggaga 5221 tctcctggaa gtatctctgg acgagactca acacatatgg cggattttgc aggccacaca 5281 cccaccctct gaagacagat tcttgcatat catggaggat gacagcatgg aagagaaacc 5341 actaaaagtg aaaggaaagg actcttcaga gaagaaacgg aaacggaagc tagaaaaggt 5401 agagcaactt tttggagaag gaaaacagaa gtccaaggag ttaaagaaaa tggacaaacc 5461 tagaaagaag aaattaaaat taggtgcaga caaatcaaag gagctgaata aactggccaa 5521 gaaactagca aaagaagaag agagaaagaa aaagaaggag aaggctgctg cagccaaagt 5581 tgaacttgtg aaagagagca ctgaaaagaa aagagagaaa aaggtgctgg acatcccctc 5641 aaagtatgac tggtcaggag cagaggagtc tgatgatgag aatgctgtgt gcgcagcaca 5701 gaactgccaa aggccctgca aggacaaggt agactgggta caatgtgatg gtggctgtga 5761 tgagtggttt catcaagttt gtgtgggtgt atctccagaa atggctgaaa atgaagatta 5821 catctgtata aactgtgcaa agaagcaggg gccagttagc ccaggtccag caccacctcc 5881 ttccttcata atgagctaca aactaccaat ggaggatctt aaagagacca gtCTCGAGGG 5941 TGGTGGCGGG AGCGGAGGTG GAGGGTCGTC AGGTGTGACC GGCTACCGGC TGTTCGAGGA 6001 GATTCTGTAA TCTAGAGTCG GGGCGGCCGG CCGCTTCGAG CAGACATGAT AAGATACATT 6061 GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT 6121 TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC 6181 AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG 6241 TAAAACCTCT ACAAATGTGG TAAAATCGAT AAGGATCCGT CGACCGATGC CCTTGAGAGC 6301 CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG CCGCACTTAT 6361 GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT TCCGCTTCCT 6421 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 6481 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 6541 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 6601 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 6661 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 6721 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 6781 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 6841 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 6901 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 6961 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 7021 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 7081 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT 7141 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 7201 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 7261 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 7321 GTATATATGA GTAAACTTGG TCTGACAGCG GCCGCAAATG CTAAACCACT GCAGTGGTTA 7381 CCAGTGCTTG ATCAGTGAGG CACCGATCTC AGCGATCTGC CTATTTCGTT CGTCCATAGT 7441 GGCCTGACTC CCCGTCGTGT AGATCACTAC GATTCGTGAG GGCTTACCAT CAGGCCCCAG 7501 CGCAGCAATG ATGCCGCGAG AGCCGCGTTC ACCGGCCCCC GATTTGTCAG CAATGAACCA 7561 GCCAGCAGGG AGGGCCGAGC GAAGAAGTGG TCCTGCTACT TTGTCCGCCT CCATCCAGTC 7621 TATGAGCTGC TGTCGTGATG CTAGAGTAAG AAGTTCGCCA GTGAGTAGTT TCCGAAGAGT 7681 TGTGGCCATT GCTACTGGCA TCGTGGTATC ACGCTCGTCG TTCGGTATGG CTTCGTTCAA 7741 CTCTGGTTCC CAGCGGTCAA GCCGGGTCAC ATGATCACCC ATATTATGAA GAAATGCAGT 7801 CAGCTCCTTA GGGCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCGGTGT TGTCGCTCAT 7861 GGTAATGGCA GCACTACACA ATTCTCTTAC CGTCATGCCA TCCGTAAGAT GCTTTTCCGT 7921 GACCGGCGAG TACTCAACCA AGTCGTTTTG TGAGTAGTGT ATACGGCGAC CAAGCTGCTC 7981 TTGCCCGGCG TCTATACGGG ACAACACCGC GCCACATAGC AGTACTTTGA AAGTGCTCAT 8041 CATCGGGAAT CGTTCTTCGG GGCGGAAAGA CTCAAGGATC TTGCCGCTAT TGAGATCCAG 8101 TTCGATATAG CCCACTCTTG CACCCAGTTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 8161 TTCGGGGTGT GCAAAAACAG GCAAGCAAAA TGCCGCAAAG AAGGGAATGA GTGCGACACG 8221 AAAATGTTGG ATGCTCATAC TCGTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 8281 CTAGTACGTC TCTCAAGGAT AAGTAAGTAA TATTAAGGTA CGGGAGGTAT TGGACAGGCC 8341 GCAATAAAAT ATCTTTATTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GAATCGATAG 8401 TACTAACATA CGCTCTCCAT CAAAACAAAA CGAAACAAAA CAAACTAGCA AAATAGGCTG 8461 TCCCCAGTGC AAGTGCAGGT GCCAGAACAT TTCTCT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.