pCMV(CAT)T7-Fluc-SB100 plasmid

pCMV(CAT)T7-Fluc-SB100 plasmid

PVT102997

pCMV(CAT)T7-Fluc-SB100 plasmid Information

Plasmid name: pCMV(CAT)T7-Fluc-SB100
Alias:
Gene lenght:
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Chl
Screening marker:
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: SV40 intron-F1
Reverse primer:
Copy number: High
Induction:

pCMV(CAT)T7-Fluc-SB100 plasmid Description

pCMV(CAT)T7-Fluc-SB100 is a plasmid suitable for protein expression in mammalian cells.

pCMV(CAT)T7-Fluc-SB100 plasmid Sequence

1 GAATTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC 61 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG 121 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG 181 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA 241 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC 301 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 361 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 421 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA 481 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA 541 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC 601 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TCGAGGAACT GAAAAACCAG 661 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG 721 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA 781 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GAATTAATAC GACTCACTAT 841 AGGGACTAGT gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta 901 tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct 961 ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga 1021 gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa 1081 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc 1141 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct 1201 caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa 1261 aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa 1321 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg 1381 ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat 1441 catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc 1501 ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac 1561 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata 1621 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag 1681 gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt 1741 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg 1801 tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg 1861 tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg 1921 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga 1981 tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc 2041 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga 2101 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt 2161 tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga 2221 atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga 2281 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga 2341 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg 2401 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat 2461 cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgggtg ggggcggttc 2521 aggtggcggt ggatccATGG GAAAATCAAA AGAAATCAGC CAAGACCTCA GAAAAAGAAT 2581 TGTAGACCTC CACAAGTCTG GTTCATCCTT GGGAGCAATT TCCAAACGCC TGGCGGTACC 2641 ACGTTCATCT GTACAAACAA TAGTACGCAA GTATAAACAC CATGGGACCA CGCAGCCGTC 2701 ATACCGCTCA GGAAGGAGAC GCGTTCTGTC TCCTAGAGAT GAACGTACTT TGGTGCGAAA 2761 AGTGCAAATC AATCCCAGAA CAACAGCAAA GGACCTTGTG AAGATGCTGG AGGAAACAGG 2821 TACAAAAGTA TCTATATCCA CAGTAAAACG AGTCCTATAT CGACATAACC TGAAAGGCCA 2881 CTCAGCAAGG AAGAAGCCAC TGCTCCAAAA CCGACATAAG AAAGCCAGAC TACGGTTTGC 2941 AACTGCACAT GGGGACAAAG ATCGTACTTT TTGGAGAAAT GTCCTCTGGT CTGATGAAAC 3001 AAAAATAGAA CTGTTTGGCC ATAATGACCA TCGTTATGTT TGGAGGAAGA AGGGGGAGGC 3061 TTGCAAGCCG AAGAACACCA TCCCAACCGT GAAGCACGGG GGTGGCAGCA TCATGTTGTG 3121 GGGGTGCTTT GCTGCAGGAG GGACTGGTGC ACTTCACAAA ATAGATGGCA TCATGGACGC 3181 GGTGCAGTAT GTGGATATAT TGAAGCAACA TCTCAAGACA TCAGTCAGGA AGTTAAAGCT 3241 TGGTCGCAAA TGGGTCTTCC AACACGACAA TGACCCCAAG CATACTTCCA AAGTTGTGGC 3301 AAAATGGCTT AAGGACAACA AAGTCAAGGT ATTGGAGTGG CCATCACAAA GCCCTGACCT 3361 CAATCCTATA GAAAATTTGT GGGCAGAACT GAAAAAGCGT GTGCGAGCAA GGAGGCCTAC 3421 AAACCTGACT CAGTTACACC AGCTCTGTCA GGAGGAATGG GCCAAAATTC ACCCAAATTA 3481 TTGTGGGAAG CTTGTGGAAG GCTACCCGAA ACGTTTGACC CAAGTTAAAC AATTTAAAGG 3541 CAATGCTACC AAATACTAGG GGCCCTAACC GCGGGGATCC AGACATGATA AGATACATTG 3601 ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT 3661 GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA 3721 ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TCGGATCCTC 3781 TAGAGTCGAC CTGCAGGCAT GCAAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 3841 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 3901 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 3961 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 4021 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 4081 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 4141 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 4201 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 4261 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 4321 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 4381 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 4441 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 4501 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 4561 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 4621 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT 4681 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 4741 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 4801 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 4861 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 4921 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 4981 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 5041 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 5101 CCAGTGCTGC AATGATACCG CTGGATCGAT CCACCATACC CACGCCGAAA CAAGCGCCCT 5161 GCACCATTAT GTTCCGGATC TGCATCGCAG GATGCTGCTG GCTACCCTGT GGAACACCTA 5221 CATCTGTATT AACGAAGCGC TAACCGTTTT TATCAGGCTC TGGGAGGCAG AATAAATGAT 5281 CATATCGTCA ATTATTACCT CCACGGGGAG AGCCTGAGCA AACTGGCCTC AGGCATTTGA 5341 GAAGCACACG GTCACACTGC TTCCGGTAGT CAATAAACCG GTAAACCAGC AATAGACATA 5401 AGCGGCTATT TAACGACCCT GCCCTGAACC GACGACCGGG TCGAATTTGC TTTCGAATTT 5461 CTGCCATTCA TCCGCTTATT ATCACTTATT CAGGCGTAGC ACCAGGCGTT TAAGGGCACC 5521 AATAACTGCC TTAAAAAAAT TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT 5581 CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAGACGGC ATGATGAACC TGAATCGCCA 5641 GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA 5701 AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG 5761 CTGAGACGAA AAACATATTC TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT 5821 AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC 5881 TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC 5941 TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG GAATTCCGGA TGAGCATTCA 6001 TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG 6061 TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG 6121 ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC 6181 CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAT AACTCAAAAA 6241 ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT GGAACCTCTT ACGTGCCGAT 6301 CAACGTCTCA TTTTCGCCAA AAGTTGGCCC AGGGCTTCCC GGTATCAACA GGGACACCAG 6361 GATTTATTTA TTCTGCGAAG TGATCTTCCG TCACAGGTAT TTATTCGGCG CAAAGTGCGT 6421 CGGGTCACTA CGTGA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.