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- LSM BIO
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- PVT10879
- 2026年03月16日
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- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pCMV (CAT)T7-SB100
- 库存：
10
- 供应商：
LSMBIO
- 规格：
质粒干粉
pCMV(CAT)T7-SB100
Catalog No. PVT10879
Packing 2ug
?
pCMV(CAT)T7-SB100 Information
Function Mammal Editing plasmids
Resistance /
Screen /
Strain /
Culture temperature
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
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文献和实验Transcriptional Activation Analysis by the Chloramphenicol Acetyl Transferase (CAT) Enzyme Assay
reporter genes can be used to measure transcription activity, such as chloramphenicol acetyl transferase (CAT), luciferase, (β-galactosidase, and human growth hormone (7 ). To examine the transcription enhancement activity of a putative promoter/enhancer
In vitro RNA synthesis from plasmid-borne sequences
ribonuclease inhibitor2μl 100mM DTT4μl 5 x T3/T7 RNA polymerase buffer4μl 2.5mM NTPsSterile, DEPC treated H2 O to a final volume of 19.5ul25U (0.5ul) T3/T7 RNA polymeraseThe reaction mixture is incubated at 37℃ for 60 minutes.5) DNA template is removed
Invitro RNA synthesis from plasmid-borne sequences
plasmid DNA 20U ribonuclease inhibitor 2μl 100mM DTT 4μl 5 x T3/T7 RNA polymerase buffer 4μl 2.5mM NTPs Sterile, DEPC treated H2 O to a final volume of 19.5ul 25U (0.5ul) T3/T7 RNA polymerase
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