pCMV-OTUD4-3×FLAG-SV40-Puro pl

pCMV-OTUD4-3×FLAG-SV40-Puro plasmid

PVT102968

pCMV-OTUD4-3×FLAG-SV40-Puro plasmid Information

Plasmid name: pCMV-OTUD4(human)-3×FLAG-SV40-Puro
Alias: NM_001366057.1;DUBA6; HIN1; HSHIN1
Gene lenght: 3342
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-OTUD4-3×FLAG-SV40-Puro plasmid Description

pCMV-OTUD4-3×FLAG-SV40-Puro is a plasmid suitable for protein expression in mammalian cells.

pCMV-OTUD4-3×FLAG-SV40-Puro plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 AAGCTTTCTA GAGAATTCGC CACCatggag gctgccgtcg gcgtccccga cggcggggac 961 cagggcggcg cggggccccg cgaggacgcg acgcccatgg acgcctatct gcggaaactg 1021 ggcttgtatc ggaaactggt cgccaaggac gggtcgtgcc tgttccgggc cgtggcggag 1081 caggtattgc actctcagtc tcgccatgtt gaagtcagaa tggcctgtat tcactatctt 1141 cgagagaaca gagagaaatt tgaagcgttt atagaaggat catttgaaga atatttaaag 1201 cgtttggaaa atccacagga atgggtagga caagtggaaa taagtgccct ttctcttatg 1261 tacaggaaag attttataat ttatcgggaa ccaaatgttt ctccttcaca agtaacagaa 1321 aataattttc ctgaaaaggt gttactgtgt ttttcaaatg gaaatcatta tgatattgtg 1381 tatcccataa agtataaaga aagctctgct atgtgtcagt ctctccttta tgaattgctg 1441 tatgagaagg tatttaaaac tgatgttagt aaaattgtga tggaactaga cacgttggaa 1501 gtagctgatg aagataacag tgaaatatca gattcagagg atgacagttg caagagtaag 1561 actgctgctg ctgctgctga tgtgaatgga tttaaacctt tgtcaggcaa tgagcagctg 1621 aagaacaatg ggaactctac tagcctgcct ttgtctagaa aggttcttaa gtcactcaat 1681 cctgcagtct atagaaatgt ggaatatgaa atttggctga agtctaaaca agctcagcaa 1741 aaacgtgatt attccattgc tgctggctta caatatgaag ttggagacaa atgtcaagtt 1801 aggttggatc acaatggaaa atttttgaat gcagatgttc aaggaattca ttctgagaat 1861 ggaccagttt tggttgaaga actgggaaag aagcacacat caaagaacct caaggcacct 1921 cccccagaaa gctggaacac agtgtcaggg aagaagatga aaaaaccttc cacttctgga 1981 caaaatttcc attctgatgt ggattacaga gggccaaaga atccaagcaa gccaataaaa 2041 gccccatcag cactacctcc tcgactgcag catccttcag gagtaagaca acatgcgttc 2101 tctagtcatt cttcagggtc acagtctcag aaattctcca gtgagcacaa aaatcttagc 2161 cggacacctt cacagatcat aagaaaacct gatcgtgaaa gagttgagga ttttgatcac 2221 acaagtcgag aatctaacta tttcggcctt tccccagaag agcgcagaga gaagcaagct 2281 atagaagaat cccgtttact ctatgagatt cagaacagag atgaacaggc tttcccagcc 2341 ctttccagct catcagtcaa tcagtcagct tctcagagta gcaatccatg tgtccagaga 2401 aaatcatcac atgtaggtga tagaaaagga agcaggcgga gaatggatac agaagaacga 2461 aaagacaaag actctattca tggacatagt cagttggata aaagacccga accaagcaca 2521 ttggagaata ttactgatga taaatatgca acagtttcat caccatcaaa gtcaaagaag 2581 ttagagtgcc cttctcctgc ggaacaaaag ccagcagaac atgtgtcttt gtcaaatcca 2641 gctccccttc tagtttctcc agaggtacat ctaactcctg cggtgccttc tttaccagcc 2701 actgtgccag cctggccaag tgaacctaca acttttggac caacaggtgt ccctgctcca 2761 attcccgttt tgtcagtgac acagactttg accactggac ctgattcagc tgtatcccaa 2821 gctcatttaa caccctctcc agttcctgtg tcaatacagg cagttaacca gcccttgatg 2881 cctttgcctc agacattgag cctttatcaa gacccactct atcctgggtt tccttgtaat 2941 gaaaagggag atcgagccat tgtaccacct tattcactgt gtcagactgg ggaggaccta 3001 cctaaagata agaatattct tcgattcttc ttcaatcttg gtgtgaaggc atacagttgt 3061 cctatgtggg ccccacattc ttacctgtac cctctgcacc aggcctacct ggcagcctgc 3121 aggatgtacc caaaggtccc tgtccctgtt tatcctcata atccctggtt ccaagaggct 3181 cctgctgctc agaatgaaag tgattgtacc tgtactgatg cccactttcc tatgcagact 3241 gaggccagtg ttaatggtca aatgccacag ccagagattg gaccgccgac attttcttca 3301 cctctggtta tccctccatc tcaggtgtct gaaagtcatg gacaattgtc ttaccaggct 3361 gatcttgaat ctgagacccc tgggcagctt ctgcatgctg attatgaaga gtcactaagt 3421 ggcaagaata tgttccccca gccatctttt ggacccaatc cattcttagg cccagttcct 3481 attgcacctc ctttctttcc tcatgtttgg tatgggtacc cttttcaggg attcatagaa 3541 aatccagtaa tgaggcagaa tattgtcctg ccctctgatg aaaaaggaga attggatctg 3601 tctctggaaa atctggatct gtctaaagat tgtggttcag tttcaacagt agatgagttt 3661 ccagaagcca ggggtgaaca tgtacattct ctccctgaag caagtgtgag cagtaagccg 3721 gacgaaggcc ggacagagca atcttcccag acacgaaagg cagatacggc attggcttcc 3781 atccctcctg tagcagaggg aaaggctcat cctcccactc agattctaaa cagagagaga 3841 gaaactgtgc ctgttgaact tgaacctaaa aggaccattc aaagcctgaa agaaaaaaca 3901 gaaaaagtaa aagatcctaa gactgctgct gatgtggtca gccctggggc caactctgtt 3961 gatagcagag tgcaaagacc aaaagaagag agttcagaag atgaaaatga agtgtctaat 4021 attttgagaa gtggtagatc caagcagttc tataatcaaa cttatggaag caggaagtac 4081 aaaagtgatt ggggctattc tggtaggggt ggatatcaac atgtgagaag tgaggagtcc 4141 tggaaaggac agccaagcag aagtcgggat gaaggttatc agtaccatcg aaatgtcaga 4201 gggcgaccat ttaggggaga taggaggaga tcagggatgg gagatggcca taggggacag 4261 cacactCTCG AGACCGGTGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC 4321 GATTACAAGG ATGACGATGA CAAGTGACCG GTaAGTTTAA ACCGCTGATC AGCCTCGACT 4381 GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG 4441 GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG 4501 AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG 4561 GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA 4621 ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 4681 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 4741 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 4801 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 4861 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 4921 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 4981 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGG CCATTTCGGC CTATTGGTTA 5041 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT 5101 TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 5161 ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA 5221 GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC 5281 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG 5341 CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG 5401 GAGGCCTAGG CTTTTGCAAA AAGCTCCCgg gatgaccgag tacaagccca cggtgcgcct 5461 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta 5521 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca 5581 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 5641 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 5701 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 5761 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 5821 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 5881 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 5941 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 6001 ctggtgcatg acccgcaagc ccggtgcctg aTTCGAAATG ACCGACCAAG CGACGCCCAA 6061 CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT 6121 CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT 6181 CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC 6241 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT 6301 CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG 6361 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC 6421 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC 6481 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT 6541 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC 6601 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 6661 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 6721 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 6781 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 6841 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 6901 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 6961 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 7021 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 7081 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 7141 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 7201 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 7261 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA 7321 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 7381 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 7441 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 7501 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG 7561 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA 7621 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC 7681 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC 7741 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC 7801 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC 7861 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC 7921 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 7981 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC 8041 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG 8101 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG 8161 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT 8221 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC 8281 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA 8341 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA 8401 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 8461 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.