pLV3-CMV-PTPA-3×FLAG-P2A-EGFP

pLV3-CMV-PTPA-3×FLAG-P2A-EGFP plasmid

PVT110332

pLV3-CMV-PTPA-3×FLAG-P2A-EGFP plasmid Information

Plasmid name: pLV3-CMV-PTPA(human)-3×FLAG-P2A-EGFP
Alias: NM_178001.3;PARK25; PP2A; PPP2R4; PR53
Gene lenght: 1074
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PTPA-3×FLAG-P2A-EGFP plasmid Description

pLV3-CMV-PTPA-3×FLAG-P2A-EGFP It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-PTPA-3×FLAG-P2A-EGFP plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 1981 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 2041 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 2101 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 2161 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 2221 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 2281 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 2341 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 2401 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat 2461 ccgctagcgc taccggactc agatctcgag gccaccatgg ctgagggcga gcggcagccg 2521 ccgccagatt cttcagagga ggcccctcca gccactcaga acttcatcat tccaaaaaag 2581 gagatccaca cagttccaga catgggcaaa tggaagcgtt ctcaggcata cgctgactac 2641 atcggattca tccttaccct caacgaaggt gtgaagggga agaagctgac cttcgagtac 2701 agagtctccg agatgtggaa tgaggttcat gaggaaaagg agcaggctgc aaagcagagt 2761 gtgtcctgcg atgaatgcat accattaccc cgcgccgggc actgtgcacc ttcggaggcc 2821 attgagaaac tagtcgctct tctcaacacg ctggacaggt ggattgatga gactcctcca 2881 gtggaccagc cctctcggtt tgggaataag gcatacagga cctggtatgc caaacttgat 2941 gaggaagcag aaaacttggt ggccacagtg gtccctaccc atctggcagc tgctgtgcct 3001 gaggtggctg tttacctaaa ggagtcagtg gggaactcca cgcgcattga ctacggcaca 3061 gggcatgagg cagccttcgc tgctttcctc tgctgtctct gcaagattgg ggtgctccgg 3121 gtggatgacc aaatagctat tgtcttcaag gtgttcaatc ggtaccttga ggttatgcgg 3181 aaactccaga aaacatacag gatggagcca gccggcagcc agggagtgtg gggtctggat 3241 gacttccagt ttctgccctt catctggggc agttcgcagc tgatagacca cccatacctg 3301 gagcccagac actttgtgga tgagaaggcc gtgaatgaga accacaagga ctacatgttc 3361 ctggagtgta tcctgtttat taccgagatg aagactggcc catttgcaga gcactccaac 3421 cagctgtgga acatcagcgc cgtcccttcc tggtccaaag tgaaccaggg tctcatccgc 3481 atgtataagg ccgagtgcct ggagaagttc cctgtgatcc agcacttcaa gttcgggagc 3541 ctgctgccca tccatcctgt cacgtcgggc gtcgacGACT ACAAAGACCA TGACGGTGAT 3601 TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGGGAAGCGG AGCCACTAAC 3661 TTCTCCCTGT TGAAACAAGC AGGGGATGTC GAAGAGAATC CCGGGCCAga attcGCCACC 3721 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 3781 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 3841 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 3901 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 3961 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 4021 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 4081 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 4141 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 4201 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 4261 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 4321 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 4381 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa 4441 GGATCCaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 4501 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 4561 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 4621 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 4681 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 4741 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 4801 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg 4861 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 4921 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 4981 cgtctccgcc ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgcctggta 5041 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 5101 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact 5161 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 5221 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 5281 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 5341 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 5401 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 5461 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 5521 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg 5581 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 5641 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca 5701 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 5761 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 5821 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 5881 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 5941 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 6001 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 6061 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 6121 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 6181 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 6241 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 6301 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 6361 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 6421 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 6481 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 6541 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 6601 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 6661 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 6721 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 6781 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 6841 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 6901 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 6961 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 7021 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 7081 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 7141 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 7201 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 7261 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 7321 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 7381 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 7441 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 7501 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 7561 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 7621 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 7681 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 7741 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 7801 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 7861 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 7921 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 7981 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 8041 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 8101 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 8161 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag 8221 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 8281 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 8341 gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.