pTLCV2-U6-PNPT1-sgRNA2-Cas9-EG

pTLCV2-U6-PNPT1-sgRNA2-Cas9-EGFP-EF1a-Puro plasmid

PVT102666

pTLCV2-U6-PNPT1-sgRNA2-Cas9-EGFP-EF1a-Puro plasmid Information

Plasmid name: pTLCV2-U6-PNPT1(human)-sgRNA2-Cas9-EGFP-EF1a-Puro
Alias: GTACGAATAGGAATAATTGA
Gene lenght:
Host: Mammalian cells
Use(s): Gene editing
Fragment type: CRISPR
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pTLCV2-U6-PNPT1-sgRNA2-Cas9-EGFP-EF1a-Puro plasmid Description

pTLCV2-U6-PNPT1-sgRNA2-Cas9-EGFP-EF1a-Puro is a plasmid suitable for gene editing in mammalian cells.

pTLCV2-U6-PNPT1-sgRNA2-Cas9-EGFP-EF1a-Puro plasmid Sequence

1 ATGTCGAGTT TACTCCCTAT CAGTGATAGA GAACGATGTC GAGTTTACTC CCTATCAGTG 61 ATAGAGAACG TATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGTTTAC 121 TCCCTATCAG TGATAGAGAA CGTATGTCGA GTTTATCCCT ATCAGTGATA GAGAACGTAT 181 GTCGAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGTCG AGGTAGGCGT GTACGGTGGG 241 AGGCCTATAT AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA ATACCGGTTC 301 TAGAGCGCTG CCACCATGGA CAAGAAGTAC AGCATCGGCC TGGACATCGG CACCAACTCT 361 GTGGGCTGGG CCGTGATCAC CGACGAGTAC AAGGTGCCCA GCAAGAAATT CAAGGTGCTG 421 GGCAACACCG ACCGGCACAG CATCAAGAAG AACCTGATCG GAGCCCTGCT GTTCGACAGC 481 GGCGAAACAG CCGAGGCCAC CCGGCTGAAG AGAACCGCCA GAAGAAGATA CACCAGACGG 541 AAGAACCGGA TCTGCTATCT GCAAGAGATC TTCAGCAACG AGATGGCCAA GGTGGACGAC 601 AGCTTCTTCC ACAGACTGGA AGAGTCCTTC CTGGTGGAAG AGGATAAGAA GCACGAGCGG 661 CACCCCATCT TCGGCAACAT CGTGGACGAG GTGGCCTACC ACGAGAAGTA CCCCACCATC 721 TACCACCTGA GAAAGAAACT GGTGGACAGC ACCGACAAGG CCGACCTGCG GCTGATCTAT 781 CTGGCCCTGG CCCACATGAT CAAGTTCCGG GGCCACTTCC TGATCGAGGG CGACCTGAAC 841 CCCGACAACA GCGACGTGGA CAAGCTGTTC ATCCAGCTGG TGCAGACCTA CAACCAGCTG 901 TTCGAGGAAA ACCCCATCAA CGCCAGCGGC GTGGACGCCA AGGCCATCCT GTCTGCCAGA 961 CTGAGCAAGA GCAGACGGCT GGAAAATCTG ATCGCCCAGC TGCCCGGCGA GAAGAAGAAT 1021 GGCCTGTTCG GAAACCTGAT TGCCCTGAGC CTGGGCCTGA CCCCCAACTT CAAGAGCAAC 1081 TTCGACCTGG CCGAGGATGC CAAACTGCAG CTGAGCAAGG ACACCTACGA CGACGACCTG 1141 GACAACCTGC TGGCCCAGAT CGGCGACCAG TACGCCGACC TGTTTCTGGC CGCCAAGAAC 1201 CTGTCCGACG CCATCCTGCT GAGCGACATC CTGAGAGTGA ACACCGAGAT CACCAAGGCC 1261 CCCCTGAGCG CCTCTATGAT CAAGAGATAC GACGAGCACC ACCAGGACCT GACCCTGCTG 1321 AAAGCTCTCG TGCGGCAGCA GCTGCCTGAG AAGTACAAAG AGATTTTCTT CGACCAGAGC 1381 AAGAACGGCT ACGCCGGCTA CATTGACGGC GGAGCCAGCC AGGAAGAGTT CTACAAGTTC 1441 ATCAAGCCCA TCCTGGAAAA GATGGACGGC ACCGAGGAAC TGCTCGTGAA GCTGAACAGA 1501 GAGGACCTGC TGCGGAAGCA GCGGACCTTC GACAACGGCA GCATCCCCCA CCAGATCCAC 1561 CTGGGAGAGC TGCACGCCAT TCTGCGGCGG CAGGAAGATT TTTACCCATT CCTGAAGGAC 1621 AACCGGGAAA AGATCGAGAA GATCCTGACC TTCCGCATCC CCTACTACGT GGGCCCTCTG 1681 GCCAGGGGAA ACAGCAGATT CGCCTGGATG ACCAGAAAGA GCGAGGAAAC CATCACCCCC 1741 TGGAACTTCG AGGAAGTGGT GGACAAGGGC GCTTCCGCCC AGAGCTTCAT CGAGCGGATG 1801 ACCAACTTCG ATAAGAACCT GCCCAACGAG AAGGTGCTGC CCAAGCACAG CCTGCTGTAC 1861 GAGTACTTCA CCGTGTATAA CGAGCTGACC AAAGTGAAAT ACGTGACCGA GGGAATGAGA 1921 AAGCCCGCCT TCCTGAGCGG CGAGCAGAAA AAGGCCATCG TGGACCTGCT GTTCAAGACC 1981 AACCGGAAAG TGACCGTGAA GCAGCTGAAA GAGGACTACT TCAAGAAAAT CGAGTGCTTC 2041 GACTCCGTGG AAATCTCCGG CGTGGAAGAT CGGTTCAACG CCTCCCTGGG CACATACCAC 2101 GATCTGCTGA AAATTATCAA GGACAAGGAC TTCCTGGACA ATGAGGAAAA CGAGGACATT 2161 CTGGAAGATA TCGTGCTGAC CCTGACACTG TTTGAGGACA GAGAGATGAT CGAGGAACGG 2221 CTGAAAACCT ATGCCCACCT GTTCGACGAC AAAGTGATGA AGCAGCTGAA GCGGCGGAGA 2281 TACACCGGCT GGGGCAGGCT GAGCCGGAAG CTGATCAACG GCATCCGGGA CAAGCAGTCC 2341 GGCAAGACAA TCCTGGATTT CCTGAAGTCC GACGGCTTCG CCAACAGAAA CTTCATGCAG 2401 CTGATCCACG ACGACAGCCT GACCTTTAAA GAGGACATCC AGAAAGCCCA GGTGTCCGGC 2461 CAGGGCGATA GCCTGCACGA GCACATTGCC AATCTGGCCG GCAGCCCCGC CATTAAGAAG 2521 GGCATCCTGC AGACAGTGAA GGTGGTGGAC GAGCTCGTGA AAGTGATGGG CCGGCACAAG 2581 CCCGAGAACA TCGTGATCGA AATGGCCAGA GAGAACCAGA CCACCCAGAA GGGACAGAAG 2641 AACAGCCGCG AGAGAATGAA GCGGATCGAA GAGGGCATCA AAGAGCTGGG CAGCCAGATC 2701 CTGAAAGAAC ACCCCGTGGA AAACACCCAG CTGCAGAACG AGAAGCTGTA CCTGTACTAC 2761 CTGCAGAATG GGCGGGATAT GTACGTGGAC CAGGAACTGG ACATCAACCG GCTGTCCGAC 2821 TACGATGTGG ACCATATCGT GCCTCAGAGC TTTCTGAAGG ACGACTCCAT CGACAACAAG 2881 GTGCTGACCA GAAGCGACAA GAACCGGGGC AAGAGCGACA ACGTGCCCTC CGAAGAGGTC 2941 GTGAAGAAGA TGAAGAACTA CTGGCGGCAG CTGCTGAACG CCAAGCTGAT TACCCAGAGA 3001 AAGTTCGACA ATCTGACCAA GGCCGAGAGA GGCGGCCTGA GCGAACTGGA TAAGGCCGGC 3061 TTCATCAAGA GACAGCTGGT GGAAACCCGG CAGATCACAA AGCACGTGGC ACAGATCCTG 3121 GACTCCCGGA TGAACACTAA GTACGACGAG AATGACAAGC TGATCCGGGA AGTGAAAGTG 3181 ATCACCCTGA AGTCCAAGCT GGTGTCCGAT TTCCGGAAGG ATTTCCAGTT TTACAAAGTG 3241 CGCGAGATCA ACAACTACCA CCACGCCCAC GACGCCTACC TGAACGCCGT CGTGGGAACC 3301 GCCCTGATCA AAAAGTACCC TAAGCTGGAA AGCGAGTTCG TGTACGGCGA CTACAAGGTG 3361 TACGACGTGC GGAAGATGAT CGCCAAGAGC GAGCAGGAAA TCGGCAAGGC TACCGCCAAG 3421 TACTTCTTCT ACAGCAACAT CATGAACTTT TTCAAGACCG AGATTACCCT GGCCAACGGC 3481 GAGATCCGGA AGCGGCCTCT GATCGAGACA AACGGCGAAA CCGGGGAGAT CGTGTGGGAT 3541 AAGGGCCGGG ATTTTGCCAC CGTGCGGAAA GTGCTGAGCA TGCCCCAAGT GAATATCGTG 3601 AAAAAGACCG AGGTGCAGAC AGGCGGCTTC AGCAAAGAGT CTATCCTGCC CAAGAGGAAC 3661 AGCGATAAGC TGATCGCCAG AAAGAAGGAC TGGGACCCTA AGAAGTACGG CGGCTTCGAC 3721 AGCCCCACCG TGGCCTATTC TGTGCTGGTG GTGGCCAAAG TGGAAAAGGG CAAGTCCAAG 3781 AAACTGAAGA GTGTGAAAGA GCTGCTGGGG ATCACCATCA TGGAAAGAAG CAGCTTCGAG 3841 AAGAATCCCA TCGACTTTCT GGAAGCCAAG GGCTACAAAG AAGTGAAAAA GGACCTGATC 3901 ATCAAGCTGC CTAAGTACTC CCTGTTCGAG CTGGAAAACG GCCGGAAGAG AATGCTGGCC 3961 TCTGCCGGCG AACTGCAGAA GGGAAACGAA CTGGCCCTGC CCTCCAAATA TGTGAACTTC 4021 CTGTACCTGG CCAGCCACTA TGAGAAGCTG AAGGGCTCCC CCGAGGATAA TGAGCAGAAA 4081 CAGCTGTTTG TGGAACAGCA CAAGCACTAC CTGGACGAGA TCATCGAGCA GATCAGCGAG 4141 TTCTCCAAGA GAGTGATCCT GGCCGACGCT AATCTGGACA AAGTGCTGTC CGCCTACAAC 4201 AAGCACCGGG ATAAGCCCAT CAGAGAGCAG GCCGAGAATA TCATCCACCT GTTTACCCTG 4261 ACCAATCTGG GAGCCCCTGC CGCCTTCAAG TACTTTGACA CCACCATCGA CCGGAAGAGG 4321 TACACCAGCA CCAAAGAGGT GCTGGACGCC ACCCTGATCC ACCAGAGCAT CACCGGCCTG 4381 TACGAGACAC GGATCGACCT GTCTCAGCTG GGAGGCGACA AGCGACCTGC CGCCACAAAG 4441 AAGGCTGGAC AGGCTAAGAA GAAGAAAGAT TACAAAGACG ATGACGATAA GGGATCCGGA 4501 GAGGGCAGAG GAAGTCTGCT AACATGCGGT GACGTCGAGG AGAATCCTGG CCCAGTGAGC 4561 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA 4621 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG 4681 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC 4741 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC 4801 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC 4861 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC 4921 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG 4981 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG 5041 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC 5101 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC 5161 ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG 5221 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTA AGCTAGCATC 5281 CGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT CGGCAATTGA 5341 TCCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT GTACTGGCTC 5401 CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC CGTGAACGTT 5461 CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGACTGCGA TCGCAATGTA CAGTATGACC 5521 GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC 5581 CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC 5641 ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC 5701 AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC 5761 GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG 5821 CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG 5881 TGGTTCCTGG CCACCGTCGG AGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC 5941 GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC 6001 TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC 6061 GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CGGTTCCGGC 6121 GCAACAAACT TCTCTCTGCT GAAACAAGCC GGAGATGTCG AAGAGAATCC TGGACCGATG 6181 TCTAGACTGG ACAAGAGCAA AGTCATAAAC GGAGCTCTGG AATTACTCAA TGGTGTCGGT 6241 ATCGAAGGCC TGACGACAAG GAAACTCGCT CAAAAGCTGG GAGTTGAGCA GCCTACCCTG 6301 TACTGGCACG TGAAGAACAA GCGGGCCCTG CTCGATGCCC TGCCAATCGA GATGCTGGAC 6361 AGGCATCATA CCCACTTCTG CCCCCTGGAA GGCGAGTCAT GGCAAGACTT TCTGCGGAAC 6421 AACGCCAAGT CATACCGCTG TGCTCTCCTC TCACATCGCG ACGGGGCTAA AGTGCATCTC 6481 GGCACCCGCC CAACAGAGAA ACAGTACGAA ACCCTGGAAA ATCAGCTCGC GTTCCTGTGT 6541 CAGCAAGGCT TCTCCCTGGA GAACGCACTG TACGCTCTGT CCGCCGTGGG CCACTTTACA 6601 CTGGGCTGCG TATTGGAGGA ACAGGAGCAT CAAGTAGCAA AAGAGGAAAG AGAGACACCT 6661 ACCACCGATT CTATGCCCCC ACTTCTGAGA CAAGCAATTG AGCTGTTCGA CCGGCAGGGA 6721 GCCGAACCTG CCTTCCTTTT CGGCCTGGAA CTAATCATAT GTGGCCTGGA GAAACAGCTA 6781 AAGTGCGAAA GCGGCGGGCC GACCGACGCC CTTGACGATT TTGACTTAGA CATGCTCCCA 6841 GCCGATGCCC TTGACGACTT TGACCTTGAT ATGCTGCCTG CTGACGCTCT TGACGATTTT 6901 GACCTTGACA TGCTCCCCGG GTAACTCGAG CGCGTTAAGT CGACAATCAA CCTCTGGATT 6961 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG 7021 GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT 7081 CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC 7141 AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA 7201 CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC 7261 TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT 7321 CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT 7381 GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC 7441 CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA 7501 CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCGTCGACT TTAAGACCAA TGACTTACAA 7561 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA 7621 CTCCCAACGA AGACAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT TAGACCAGAT 7681 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT 7741 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC 7801 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGG GCCCGTTTAA ACCCGCTGAT 7861 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT 7921 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT 7981 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG 8041 GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG 8101 AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT 8161 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG 8221 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC 8281 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC 8341 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT 8401 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA 8461 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG 8521 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA 8581 TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 8641 CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG 8701 AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC 8761 CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT 8821 TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG 8881 AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT 8941 CGGATCTGAT CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA 9001 TACGACAAGG TGAGGAACTA AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC 9061 GCGCGCGACG TCGCCGGAGC GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC 9121 TTCGTGGAGG ACGACTTCGC CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG 9181 GTCCAGGACC AGGTGGTGCC GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC 9241 GAGCTGTACG CCGAGTGGTC GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG 9301 GCCATGACCG AGATCGGCGA GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC 9361 GGCAACTGCG TGCACTTCGT GGCCGAGGAG CAGGACTGAC ACGTGCTACG AGATTTCGAT 9421 TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG 9481 ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT 9541 GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT 9601 TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT 9661 ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA 9721 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC 9781 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC 9841 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC 9901 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT 9961 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA 10021 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA 10081 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT 10141 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 10201 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC 10261 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT 10321 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC 10381 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG 10441 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 10501 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC 10561 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 10621 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA 10681 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 10741 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 10801 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT 10861 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA 10921 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC 10981 AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC 11041 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG 11101 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC 11161 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC 11221 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG 11281 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC 11341 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT 11401 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC 11461 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC 11521 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC 11581 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC 11641 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA 11701 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 11761 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 11821 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC 11881 CCCTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATCTGC 11941 TCCCTGCTTG TGTGTTGGAG GTCGCTGAGT AGTGCGCGAG CAAAATTTAA GCTACAACAA 12001 GGCAAGGCTT GACCGACAAT TGCATGAAGA ATCTGCTTAG GGTTAGGCGT TTTGCGCTGC 12061 TTCGCGATGT ACGGGCCAGA TATACGCGTT GACATTGATT ATTGACTAGT TATTAATAGT 12121 AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA 12181 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA 12241 CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT 12301 TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA 12361 TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG 12421 ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT 12481 TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC 12541 ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT 12601 GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT 12661 ATATAAGCAG CGCGTTTTGC CTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG 12721 GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG 12781 CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC 12841 TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG 12901 GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG 12961 GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA 13021 GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA 13081 TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA 13141 GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC 13201 AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT 13261 ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG 13321 AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGCGG 13381 CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT 13441 AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA 13501 GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA 13561 GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA 13621 TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT 13681 CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA 13741 AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC 13801 ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT 13861 CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC 13921 TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT 13981 AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA 14041 TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA 14101 GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG 14161 AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA 14221 TCCATTCGAT TAGTGAACGG ATCGGCACTG CGTGCGCCAA TTCTGCAGAC AAATGGCAGT 14281 ATTCATCCAC AATTTTAAAA GAAAAGGGGG GATTGGGGGG TACAGTGCAG GGGAAAGAAT 14341 AGTAGACATA ATAGCAACAG ACATACAAAC TAAAGAATTA CAAAAACAAA TTACAAAAAT 14401 TCAAAATTTT CGGGTTTATT ACAGGGACAG CAGAGATCCA GTTTGGTTAA TTAAGGTACC 14461 GAGGGCCTAT TTCCCATGAT TCCTTCATAT TTGCATATAC GATACAAGGC TGTTAGAGAG 14521 ATAATTAGAA TTAATTTGAC TGTAAACACA AAGATATTAG TACAAAATAC GTGACGTAGA 14581 AAGTAATAAT TTCTTGGGTA GTTTGCAGTT TTAAAATTAT GTTTTAAAAT GGACTATCAT 14641 ATGCTTACCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 14701 CGAAACACCG GTACGAATAG GAATAATTGA GTTTTAGAGC TAGAAATAGC AAGTTAAAAT 14761 AAGGCTAGTC CGTTATCAAC TTGAAAAAGT GGCACCGAGT CGGTGCTTTT TTGAATTCAC 14821 TTTGGCCGCG AATCGATATG TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.