pLV3-CMV-MANF-3×FLAG-CopGFP-Pu

pLV3-CMV-MANF-3×FLAG-CopGFP-Puro plasmid

PVT99044

pLV3-CMV-MANF-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-MANF(human)-3×FLAG-CopGFP-Puro
Alias: NM_006010.6;ARMET; ARP
Gene lenght: 546bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MANF-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-MANF-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MANF-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga ggaggatgtg ggccacgcag 2341 gggctggcgg tggcgctggc tctgagcgtg ctgccgggca gccgggcgct gcggccgggc 2401 gactgcgaag tttgtatttc ttatctggga agattttacc aggacctcaa agacagagat 2461 gtcacattct caccagccac tattgaaaac gaacttataa agttctgccg ggaagcaaga 2521 ggcaaagaga atcggttgtg ctactatatc ggggccacag atgatgcagc caccaaaatc 2581 atcaatgagg tatcaaagcc tctggcccac cacatccctg tggagaagat ctgtgagaag 2641 cttaagaaga aggacagcca gatatgtgag cttaagtatg acaagcagat cgacctgagc 2701 acagtggacc tgaagaagct ccgagttaaa gagctgaaga agattctgga tgactggggg 2761 gagacatgca aaggctgtgc agaaaagtct gactacatcc ggaagataaa tgaactgatg 2821 cctaaatatg cccccaaggc agccagtgca cggaccgatt tgggatccGA CTACAAAGAC 2881 CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGtagcgg 2941 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 3001 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 3061 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 3121 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 3181 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 3241 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 3301 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 3361 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 3421 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 3481 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag 3541 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga 3601 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg 3661 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc 3721 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac 3781 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac 3841 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc 3901 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg 3961 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac 4021 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg 4081 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag 4141 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc 4201 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc 4261 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc 4321 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 4381 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 4441 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 4501 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 4561 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 4621 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 4681 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 4741 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 4801 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 4861 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa 4921 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 4981 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 5041 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 5101 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 5161 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 5221 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 5281 actgacaatt ccgtggtgtt gtcggggaaA TCATCGTCCT TTCCTtggct gctcgcctgt 5341 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 5401 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctTcgcctt 5461 cgccctcaga cgagtcggat ctccctttgg Gccgcctccc cgcctggtac ctttaagacc 5521 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 5581 agggctaatt cactcccaac gaaGataaga tctgcttttt gcttgtactg ggtctctctg 5641 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 5701 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 5761 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 5821 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 5881 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 5941 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 6001 ttatcatgtc tggctctagc taTCCCGCCC CTAACTCCGC CCAtcccgcc cctaactccg 6061 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 6121 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 6181 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta 6241 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 6301 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 6361 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 6421 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 6481 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 6541 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 6601 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 6661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 6721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 6781 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 6841 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 6901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 6961 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 7021 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 7081 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 7141 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 7201 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 7261 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 7321 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 7381 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 7441 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 7501 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 7561 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 7621 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 7681 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 7741 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 7801 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 7861 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 7921 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 7981 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 8041 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 8101 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 8161 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 8221 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 8281 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 8341 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 8401 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 8461 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 8521 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 8581 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 8641 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 8701 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 8761 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 8821 ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.