pLV3-CMV-MCS-3×FLAG-Fluc-Hyg p

pLV3-CMV-MCS-3×FLAG-Fluc-Hyg plasmid

PVT96549

pLV3-CMV-MCS-3×FLAG-Fluc-Hyg plasmid Information

Plasmid name: pLV3-CMV-MCS-3×FLAG-Fluc-Hyg
Alias: -
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MCS-3×FLAG-Fluc-Hyg plasmid Description

pLV3-CMV-MCS-3×FLAG-Fluc-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MCS-3×FLAG-Fluc-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagaGC 2281 TAGCGTGATA TCCAGCTCGA GGGATCCGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 2341 CATGACATCG ACTACAAGGA TGACGATGAC AAGtagcggc cgcaaggatc tgcgatcgct 2401 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2461 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2521 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2581 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag 2641 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt 2701 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg 2761 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta 2821 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt 2881 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagacgcca 2941 ccatggagag cgacgagagc ggcctgatgg aagacgccaa aaacataaag aaaggcccgg 3001 cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga 3061 gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca 3121 cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc 3181 tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg 3241 tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac 3301 gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg 3361 ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca 3421 tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc 3481 tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa 3541 ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc 3601 atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca 3661 ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta 3721 cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc 3781 tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat 3841 tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa 3901 ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc 3961 atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta 4021 cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga 4081 aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg 4141 tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga 4201 ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact 4261 tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg 4321 ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc 4381 ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga 4441 cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt 4501 tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg 4561 caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgagat 4621 cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc tccaccggat 4681 ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta 4741 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca 4801 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg 4861 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc 4921 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg 4981 gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc 5041 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg 5101 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa 5161 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc 5221 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc 5281 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct 5341 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga 5401 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt 5461 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc 5521 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg 5581 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag 5641 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct 5701 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg 5761 aataggtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 5821 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 5881 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 5941 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 6001 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 6061 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 6121 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc 6181 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 6241 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 6301 ttccgcgtct ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc 6361 tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 6421 aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt 6481 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 6541 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 6601 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 6661 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa 6721 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata 6781 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 6841 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag 6901 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 6961 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt 7021 ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 7081 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 7141 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 7201 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 7261 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 7321 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 7381 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 7441 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 7501 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 7561 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 7621 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 7681 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 7741 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 7801 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 7861 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 7921 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 7981 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 8041 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 8101 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 8161 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 8221 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 8281 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 8341 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 8401 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 8461 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 8521 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 8581 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 8641 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 8701 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 8761 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 8821 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 8881 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 8941 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 9001 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 9061 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 9121 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 9181 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 9241 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 9301 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 9361 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 9421 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 9481 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 9541 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 9601 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 9661 ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.