pCMV-EGFR-L858R-ECFP-Neo plasm

pCMV-EGFR-L858R-ECFP-Neo plasmid

PVT94696

pCMV-EGFR-L858R-ECFP-Neo plasmid Information

Plasmid name: pCMV-EGFR(human)-L858R-ECFP-Neo
Alias: NM_005228.5;ERBB; ERBB1; ERRP; HER1; mENA; NISBD2;NNCIS; PIG61;
Gene lenght: 3630bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-EGFR-L858R-ECFP-Neo plasmid Description

pCMV-EGFR-L858R-ECFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFR-L858R-ECFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagcc accatgcgac cctccgggac ggccggggca gcgctcctgg 661 cgctgctggc tgcgctctgc ccggcgagtc gggctctgga ggaaaagaaa gtttgccaag 721 gcacgagtaa caagctcacg cagttgggca cttttgaaga tcattttctc agcctccaga 781 ggatgttcaa taactgtgag gtggtccttg ggaatttgga aattacctat gtgcagagga 841 attatgatct ttccttctta aagaccatcc aggaggtggc tggttatgtc ctcattgccc 901 tcaacacagt ggagcgaatt cctttggaaa acctgcagat catcagagga aatatgtact 961 acgaaaattc ctatgcctta gcagtcttat ctaactatga tgcaaataaa accggactga 1021 aggagctgcc catgagaaat ttacaggaaa tcctgcatgg cgccgtgcgg ttcagcaaca 1081 accctgccct gtgcaacgtg gagagcatcc agtggcggga catagtcagc agtgactttc 1141 tcagcaacat gtcgatggac ttccagaacc acctgggcag ctgccaaaag tgtgatccaa 1201 gctgtcccaa tgggagctgc tggggtgcag gagaggagaa ctgccagaaa ctgaccaaaa 1261 tcatctgtgc ccagcagtgc tccgggcgct gccgtggcaa gtcccccagt gactgctgcc 1321 acaaccagtg tgctgcaggc tgcacaggcc cccgggagag cgactgcctg gtctgccgca 1381 aattccgaga cgaagccacg tgcaaggaca cctgcccccc actcatgctc tacaacccca 1441 ccacgtacca gatggatgtg aaccccgagg gcaaatacag ctttggtgcc acctgcgtga 1501 agaagtgtcc ccgtaattat gtggtgacag atcacggctc gtgcgtccga gcctgtgggg 1561 ccgacagcta tgagatggag gaagacggcg tccgcaagtg taagaagtgc gaagggcctt 1621 gccgcaaagt gtgtaacgga ataggtattg gtgaatttaa agactcactc tccataaatg 1681 ctacgaatat taaacacttc aaaaactgca cctccatcag tggcgatctc cacatcctgc 1741 cggtggcatt taggggtgac tccttcacac atactcctcc tctggatcca caggaactgg 1801 atattctgaa aaccgtaaag gaaatcacag ggtttttgct gattcaggct tggcctgaaa 1861 acaggacgga cctccatgcc tttgagaacc tagaaatcat acgcggcagg accaagcaac 1921 atggtcagtt ttctcttgca gtcgtcagcc tgaacataac atccttggga ttacgctccc 1981 tcaaggagat aagtgatgga gatgtgataa tttcaggaaa caaaaatttg tgctatgcaa 2041 atacaataaa ctggaaaaaa ctgtttggga cctccggtca gaaaaccaaa attataagca 2101 acagaggtga aaacagctgc aaggccacag gccaggtctg ccatgccttg tgctcccccg 2161 agggctgctg gggcccggag cccagggact gcgtctcttg ccggaatgtc agccgaggca 2221 gggaatgcgt ggacaagtgc aaccttctgg agggtgagcc aagggagttt gtggagaact 2281 ctgagtgcat acagtgccac ccagagtgcc tgcctcaggc catgaacatc acctgcacag 2341 gacggggacc agacaactgt atccagtgtg cccactacat tgacggcccc cactgcgtca 2401 agacctgccc ggcaggagtc atgggagaaa acaacaccct ggtctggaag tacgcagacg 2461 ccggccatgt gtgccacctg tgccatccaa actgcaccta cggatgcact gggccaggtc 2521 ttgaaggctg tccaacgaat gggcctaaga tcccgtccat cgccactggg atggtggggg 2581 ccctcctctt gctgctggtg gtggccctgg ggatcggcct cttcatgcga aggcgccaca 2641 tcgttcggaa gcgcacgctg cggaggctgc tgcaggagag ggagcttgtg gagcctctta 2701 cacccagtgg agaagctccc aaccaagctc tcttgaggat cttgaaggaa actgaattca 2761 aaaagatcaa agtgctgggc tccggtgcgt tcggcacggt gtataaggga ctctggatcc 2821 cagaaggtga gaaagttaaa attcccgtcg ctatcaagga attaagagaa gcaacatctc 2881 cgaaagccaa caaggaaatc ctcgatgaag cctacgtgat ggccagcgtg gacaaccccc 2941 acgtgtgccg cctgctgggc atctgcctca cctccaccgt gcagctcatc acgcagctca 3001 tgcccttcgg ctgcctcctg gactatgtcc gggaacacaa agacaatatt ggctcccagt 3061 acctgctcaa ctggtgtgtg cagatcgcaa agggcatgaa ctacttggag gaccgtcgct 3121 tggtgcaccg cgacctggca gccaggaacg tactggtgaa aacaccgcag catgtcaaga 3181 tcacagattt tgggAGGgcc aaactgctgg gtgcggaaga gaaagaatac catgcagaag 3241 gaggcaaagt gcctatcaag tggatggcat tggaatcaat tttacacaga atctataccc 3301 accagagtga tgtctggagc tacggggtga ctgtttggga gttgatgacc tttggatcca 3361 agccatatga cggaatccct gccagcgaga tctcctccat cctggagaaa ggagaacgcc 3421 tccctcagcc acccatatgt accatcgatg tctacatgat catggtcaag tgctggatga 3481 tagacgcaga tagtcgccca aagttccgtg agttgatcat cgaattctcc aaaatggccc 3541 gagaccccca gcgctacctt gtcattcagg gggatgaaag aatgcatttg ccaagtccta 3601 cagactccaa cttctaccgt gccctgatgg atgaagaaga catggacgac gtggtggatg 3661 ccgacgagta cctcatccca cagcagggct tcttcagcag cccctccacg tcacggactc 3721 ccctcctgag ctctctgagt gcaaccagca acaattccac cgtggcttgc attgatagaa 3781 atgggctgca aagctgtccc atcaaggaag acagcttctt gcagcgatac agctcagacc 3841 ccacaggcgc cttgactgag gacagcatag acgacacctt cctcccagtg cctgaataca 3901 taaaccagtc cgttcccaaa aggcccgctg gctctgtgca gaatcctgtc tatcacaatc 3961 agcctctgaa ccccgcgccc agcagagacc cacactacca ggacccccac agcactgcag 4021 tgggcaaccc cgagtatctc aacactgtcc agcccacctg tgtcaacagc acattcgaca 4081 gccctgccca ctgggcccag aaaggcagcc accaaattag cctggacaac cctgactacc 4141 agcaggactt ctttcccaag gaagccaagc caaatggcat ctttaagggc tccacagctg 4201 aaaatgcaga atacctaagg gtcgcgccac aaagcagtga atttattgga gcaggaggtg 4261 gaggttcaga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg 4321 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 4381 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 4441 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc gtgcagtgct 4501 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 4561 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 4621 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 4681 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc cacaacgtct 4741 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca 4801 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 4861 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 4921 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 4981 tcggcatgga cgagctgtac aagtagcggc cgcgactcta gatcataatc agccatacca 5041 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac 5101 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat 5161 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 5221 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta 5281 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 5341 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg 5401 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 5461 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc 5521 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 5581 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 5641 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 5701 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 5761 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 5821 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt 5881 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 5941 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 6001 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 6061 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 6121 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 6181 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt 6241 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 6301 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 6361 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac 6421 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 6481 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 6541 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 6601 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 6661 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 6721 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag 6781 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 6841 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 6901 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 6961 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 7021 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat 7081 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 7141 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 7201 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga 7261 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg 7321 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg 7381 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg 7441 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga 7501 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 7561 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 7621 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 7681 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 7741 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 7801 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 7861 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 7921 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 7981 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 8041 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 8101 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 8161 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 8221 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 8281 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.