pLV3-CMV-MCS-3×FLAG-mCherry-Hy

pLV3-CMV-MCS-3×FLAG-mCherry-Hyg plasmid

PVT90444

pLV3-CMV-MCS-3×FLAG-mCherry-Hyg plasmid Information

Plasmid name: pLV3-CMV-MCS-3×FLAG-mCherry-Hyg
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MCS-3×FLAG-mCherry-Hyg plasmid Description

pLV3-CMV-MCS-3×FLAG-mCherry-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MCS-3×FLAG-mCherry-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcA AGCTTggatc cGAATTCCTC GAGatgGACT ACAAAGACCA TGACGGTGAT 2341 TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGTAGgcggc cgcGaaggat 2401 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa 2461 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg 2521 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat 2581 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct 2641 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca 2701 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc 2761 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg 2821 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta 2881 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac 2941 gctagacgcc accATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT 3001 CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG 3061 CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG 3121 TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC 3181 CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT 3241 CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC 3301 CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC 3361 CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA 3421 CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG 3481 CCACTACGAC GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG 3541 CGCCTACAAC GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT 3601 GGAACAGTAC GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA 3661 GGTCGAGGGA AGCGGAGCTA CTAACTTCAG CCTGCTGAAG CAGGCTGGAG ACGTGGAGGA 3721 GAACCCTGGA CCTGTCGACa tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt 3781 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc 3841 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc 3901 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat 3961 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg 4021 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc 4081 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg 4141 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat 4201 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt 4261 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct 4321 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt 4381 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt 4441 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc 4501 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact 4561 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga 4621 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc 4681 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag 4741 cactcgtccg agggcaaagg aatagTCTAG Aaatcaacct ctggattaca aaatttgtga 4801 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 4861 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 4921 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 4981 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 5041 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 5101 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 5161 ggggaaATCA TCGTCCTTTC CTtggctgct cgcctgtgtt gccacctgga ttctgcgcgg 5221 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 5281 gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc 5341 cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag 5401 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 5461 gataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 5521 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 5581 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 5641 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt 5701 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata 5761 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 5821 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat 5881 cccgccccta actccgccAT CCCGCCCTAA CTCCGCcagt tccgcccatt ctccgcccca 5941 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 6001 ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc 6061 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 6121 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 6181 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 6241 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 6301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 6361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 6421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 6481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 6541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 6601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 6661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 6721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 6781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 6841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 6901 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 6961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 7021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 7081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 7141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 7201 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 7261 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 7321 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 7381 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 7441 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 7501 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 7561 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 7621 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 7681 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 7741 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 7801 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 7861 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 7921 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 7981 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 8041 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 8101 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 8161 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 8221 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 8281 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 8341 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 8401 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 8461 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 8521 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 8581 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 8641 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.