pBV220-PD-L1-6×His plasmid

pBV220-PD-L1-6×His plasmid

PVT98636

pBV220-PD-L1-6×His plasmid Information

Plasmid name: pBV220-PD-L1(mouse)-6×His
Alias: -
Gene lenght: 390bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: PL,PR
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pBV220-PD-L1(mouse)：CACTGACCACCATGAAGGTGAC
Reverse primer:
Copy number: High
Induction:

pBV220-PD-L1-6×His plasmid Description

pBV220-PD-L1-6×His is a plasmid suitable for protein expression in Escherichia coli.

pBV220-PD-L1-6×His plasmid Sequence

1 AGGGATAAAT ATCTAACACC GTGCGTGTTG ACTATTTTAC CTCTGGCGGT GATAATGGTT 61 GCATGTACTA AGGAGGTTGT ATGGAACAAC GCATAACCCT GAAAGATTAT GCAATGCGCT 121 TTGGGCAAAC CAAGACAGCT AAAGATCTCT CACCTACCAA ACAATGCCCC CCTGCAAAAA 181 ATAAATTCAT ATAAAAAACA TACAGATAAC CATCTGCGGT GATAAATTAT CTCTGGCGGT 241 GTTGACATAA ATACCACTGG CGGTGATACT GAGCACATCA GCAGGACGCA CTGACCACCA 301 TGAAGGTGAC GCTCTTAAAA ATTAAGCCCT GAAGAAGGGC AGCATTCAAA GCAGAAGGCT 361 TTGGGGTGTG TGATACGAAA CGAAGCATTG GTTAAAAATT AAGGAGGGAA TTCATGGCGC 421 AGGTGCAGCT GGTGGAAACC GGCGGCGGCC TGGTGCAGCC GGGCGGCAGC CTGCGTCTGA 481 GCTGCACCGC GAGCGGCTTT ACCTTTTCAA TGCACGCGAT GACCTGGTAT CGTCAGGCGC 541 CGGGCAAACA GCGCGAACTG GTGGCGGTGA TTACCTCGCA TGGCGATCGC GCGAACTATA 601 CCGATAGCGT GCGCGGCCGC TTTACCATTA GCCGTGATAA TACCAAAAAT ATGGTTTACC 661 TGCAGATGAA TAGCCTGAAA CCGGAAGATA CCGCGGTGTA TTATTGCAAT GTTCCGCGCT 721 ACGATAGCTG GGGTCAGGGC ACCCAGGTTA CCGTGAGCAG CGGCGGCCTG CCGGAAACCG 781 GCGGCCATCA TCATCATCAT CACGGATCCG TCGACCATCA TCACCATCAC CATTGACTGC 841 AGCCAAGCTT GGCTGTTTTG GCGGATGAGA GAAGATTTTC AGCCTGATAC AGATTAAATC 901 AGAACGCAGA AGCGGTCTGA TAAAACAGAA TTTGCCTGGC GGCAGTAGCG CGGTGGTCCC 961 ACCTGACCCC ATGCCGAACT CAGAAGTGAA ACGCCGTAGC GCCGATGGTA GTGTGGGGTC 1021 TCCCCATGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG 1081 ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC 1141 CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG AGGGTGGCGG GCAGGACGCC 1201 CGCCATAAAC TGCCAGGCAT CAAATTAAGC AGAAGGCCAT CCTGACGGAT GGCCTTTTTG 1261 CGTTTCTACA AACTCTTTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG 1321 AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA 1381 CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC 1441 CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC 1501 ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT 1561 CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC 1621 GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA 1681 CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC 1741 ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG 1801 GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA 1861 CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG 1921 GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA 1981 TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG 2041 GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT 2101 GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT 2161 CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG 2221 CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT 2281 TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT 2341 TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT 2401 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA 2461 GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC 2521 AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC 2581 AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT 2641 GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG 2701 GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC 2761 TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG 2821 AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG 2881 CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT 2941 GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC 3001 GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG 3061 TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC 3121 CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCTTATCT 3181 TTCCCTTTAT TTTTGCTGCG GTAAGTCGCA TAAAAACCAT TCTTCATAAT TCAATCCATT 3241 TACTATGTTA TGTTCTGAGG GGAGTGAAAA TTCCCCTAAT TCGATGAAGA TTCTTGCTCA 3301 ATTGTTATCA GCTATGCGCC GACCAGAACA CCTTGCCGAT CAGCCAAACG TCTCTTCAGG 3361 CCACTGACTA GCGATAACTT TCCCCACAAC GGAACAACTC TCATTGCATG GGATCATTGG 3421 GTACTGTGGG TTTAGTGGTT GTAAAAACAC CTGACCGCTA TCCCTGATCA GTTTCTTGAA 3481 GGTAAACTCA TCACCCCCAA GTCTGGCTAT GCAGAAATCA CCTGGCTCAA CAGCCTGCTC 3541 AGGGTCAACG AGAATTAACA TTCCGTCAGG AAAGCTTGGC TTGGAGCCTG TTGGTGCGGT 3601 CATGGAATTA CCTTCAACCT CAAGCCAGAA TGCAGAATCA CTGGCTTTTT TGGTTGTGCT 3661 TACCCATCTC TCCGCATCAC CTTTGGTAAA GGTTCTAAGC TTAGGTGAGA ACATCCCTGC 3721 CTGAACATGA GAAAAAACAG GGTACTCATA CTCACTTCTA AGTGACGGCT GCATACTAAC 3781 CGCTTCATAC ATCTCGTAGA TTTCTCTGGC GATTGAAGGG CTAAATTCTT CAACGCTAAC 3841 TTTGAGAATT TTTGTAAGCA ATGCGGCGTT ATAAGCATTT AATGCATTGA TGCCATTAAA 3901 TAAAGCACCA ACGCCTGACT GCCCCATCCC CATCTTGTCT GCGACAGATT CCTGGGATAA 3961 GCCAAGTTCA TTTTTCTTTT TTTCATAAAT TGCTTTAAGG CGACGTGCGT CCTCAAGCTG 4021 CTCTTGTGTT AATGGTTTCT TTTTTGTGCT CATACGTTAA ATCTATCACC GCA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.