pmiR362-Fluc plasmid

pmiR362-Fluc plasmid

PVT97200

pmiR362-Fluc plasmid Information

Plasmid name: pmiR362(human)-Fluc
Alias: -
Gene lenght: 2000bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: miR362
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Fluc
Reverse primer:
Copy number: High
Induction:

pmiR362-Fluc plasmid Description

pmiR362-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pmiR362-Fluc plasmid Sequence

1 agcggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa 61 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa 121 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctatcgata ggtaccGAGC 181 TTTTTGAATG CCTGTTGGAT TTGGGGATCC AATAGTGGCA AGAGCATCAG GGACCCTGGT 241 TTTACTGTGG GGTGAGCTTG TGCCAGAGAA CACAGTTCAT CATTCCCATT CAGCCTGCCT 301 TCATGTGTTC AAGGGGCTGG AAAAGGGGAG CTTTTTCTTT CCTAACTCCC ATGCTATCCT 361 TGGGGTACAG GTGCCTGAGC CTTCTCATTT GCTCCTTCTC TTTAATCCTT GATACTGGTA 421 CTAGTGCTCT CCACATGCAA TGCACCTTGG CAGGGATTCA GATGGGGTGA GCCTTAGCTG 481 CAGATGGAAG GACAGAGCCC TTGTACATCG CCTACCTCTC CTGCTGCCTG CCAGTATTAT 541 TTACTCCTGT AGGGAGCCAG AAGAGAACAG TACAGCCACA GTCCCCTGAG TGTACTGCCT 601 AGCTAGCACA TATAGGAGGA TAAAGCTCAT TTGTACTCAC TGTGCAGGTG TGCACACATG 661 CTGGGGATAC TGGAGAATGA ATTTACTTTA GTTCCCACCC CCCAAGCACC CCACCTTGTC 721 CCCCAGGATG GTCAGGCATC TAGGGATGTA GACCTGTATA CACGGAGTCC CACTGATCAG 781 GTAAGCAGTG GGTGGTTCCT TCCTGCCCTA ACTCTACTCT CTTTGCCCTC CCAGAAAGGG 841 CAGTGAGCTG TCTCGGCATT CTTCCCTTAG GTAGCTGTTG AATGAAGATG CACTTCCTTG 901 TTAATGTTCT CTCTCACAAT CACAGATACA CAAATACTCG TATATGCACA TGTGCACAAG 961 CAAGGCATAC AGGAGAATGA GCATGCAAGG GAGGCCCACA TGTGGCTGGG TGCCCAGCAT 1021 ATGCAGACAT GTGAGCATAC AGGGACAGGA GAGACAGGAG CCTTTTTGAT TATACTCACA 1081 GGGCCAGATG CCCCCATCTT TACTCATTTT CTGTCTCTCT CTCTCTCTCT TTCTCTCTCT 1141 CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTGTCACACA CACACACACA CACACACACA 1201 CACACACAGA GAGAGTGGAA CTTCTGATGT TTTCCTTCAT ATCCCTTATG TGAAGGGGCG 1261 TGAACTTTGT TGACCTAGGG AGATTGAAGG AGAACCTCAT CAACTCACAC GTGAGGAATT 1321 TAAGAGGATG AATCTGTGTA TCCCCTTGTG CAGGCACTCA CACTCACAGA TACTCGGACC 1381 TCTTCTCTCT CACCTGTCCC TTGCATGCAG GAGTTTCACC ATCCCCCTTA AAAATGCACA 1441 TTCATTCAAA CACATATATA CATAAAAATA GAGAGTAAGA GGTAAAAATA TTGACGGCTG 1501 TGTGCCAGGT ACTATACTTT ATTTCATTTT CTTGACACCA TTTAAGAGGT AGGCTTTAAA 1561 TTGTCCTCAT TCTTCAAAAG TGTTTAACTT GACCTTGGGA AAATCAGGTC GATTCTCTAG 1621 GCTAATATGT GGTAGAGCGT GCATTTGAAC CAAGGTTCGT AGATACCAAA GTGCCTGGTT 1681 TTTCCACTAC ATTAGTGATT CTCCCAGTGT AGTCCCTGGA CCAGTAGCAT CAGTATCACC 1741 TGAGAACTTG TTAGAAATGC AAATTCTTGG GTCTCACCCC AGACTCACCA AGTCAGAAAC 1801 CTCTGTAACC TGTATATTAC CCAGGTCTTC AGATGATTCT GGTGCATGTT AGAGAACAAC 1861 TGTGCTAAAC TGTACTGCCT TCTTGTTGCA TAGTCACACA AAGCATTTGC GGGATCATGA 1921 GCTTTGGCGT TATATTCATT CTCTCTTTCC TCCCTTTCAC ACTCCTCTCT CCTCCCAGTC 1981 TCTTTATAGG TTTATACTTT TTTGTTGTTA CTATCTGAGA GAACAGACAA AATGTGTGTT 2041 TAACTAGACA TCTTTAATGC AAAGCTCAAG AACTGTTCTC TTTACTTCTA TATCATGACA 2101 AGTAAGTACC TTGTGTGAAG AAGAATGAGC CCTCTCACTG AACATTCACA GGGCTTTGTG 2161 TTCTGCTCCC CCTCTCaagc ttggcattcc ggtactgttg gtaaagccac catggaagac 2221 gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga 2281 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca 2341 gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg 2401 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa 2461 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg 2521 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct 2581 accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc 2641 ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg 2701 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca 2761 gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt 2821 ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga 2881 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc 2941 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc 3001 ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa 3061 agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa 3121 tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc 3181 ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact 3241 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt 3301 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc 3361 gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac 3421 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata 3481 gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag 3541 tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac 3601 atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc 3661 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc 3721 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg 3781 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag 3841 aagggcggaa agatcgccgt gtaattctag agtcggggcg gccggccgct tcgagcagac 3901 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc 3961 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa 4021 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag 4081 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc 4141 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat 4201 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc 4261 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 4321 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 4381 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 4441 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 4501 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 4561 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 4621 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc 4681 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 4741 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 4801 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 4861 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 4921 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 4981 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 5041 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 5101 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 5161 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 5221 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 5281 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 5341 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 5401 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 5461 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 5521 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 5581 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 5641 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 5701 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 5761 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 5821 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 5881 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 5941 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 6001 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 6061 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 6121 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 6181 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 6241 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 6301 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 6361 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 6421 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 6481 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 6541 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 6601 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcccattcgc 6661 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 6721 agcccaagct accatgataa gtaagtaata ttaaggtacg ggaggtactt gg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.