pCMV-ITGB5-3×Myc-Neo plasmid

pCMV-ITGB5-3×Myc-Neo plasmid

PVT110391

pCMV-ITGB5-3×Myc-Neo plasmid Information

Plasmid name: pCMV-ITGB5(human)-3×Myc-Neo
Alias: NM_002213.5
Gene lenght: 2397
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-ITGB5-3×Myc-Neo plasmid Description

pCMV-ITGB5-3×Myc-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-ITGB5-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgcc gcgggccccg 721 gcgccgctgt acgcctgcct cctggggctc tgcgcgctcc tgccccggct cgcaggtctc 781 aacatatgca ctagtggaag tgccacctca tgtgaagaat gtctgctaat ccacccaaaa 841 tgtgcctggt gctccaaaga ggacttcgga agcccacggt ccatcacctc tcggtgtgat 901 ctgagggcaa accttgtcaa aaatggctgt ggaggtgaga tagagagccc agccagcagc 961 ttccatgtcc tgaggagcct gcccctcagc agcaagggtt cgggctctgc aggctgggac 1021 gtcattcaga tgacaccaca ggagattgcc gtgaacctcc ggcccggtga caagaccacc 1081 ttccagctac aggttcgcca ggtggaggac tatcctgtgg acctgtacta cctgatggac 1141 ctctccctgt ccatgaagga tgacttggac aatatccgga gcctgggcac caaactcgcg 1201 gaggagatga ggaagctcac cagcaacttc cggttgggat ttgggtcttt tgttgataag 1261 gacatctctc ctttctccta cacggcaccg aggtaccaga ccaatccgtg cattggttac 1321 aagttgtttc caaattgcgt cccctccttt gggttccgcc atctgctgcc tctcacagac 1381 agagtggaca gcttcaatga ggaagttcgg aaacagaggg tgtcccggaa ccgagatgcc 1441 cctgaggggg gctttgatgc agtactccag gcagccgtct gcaaggagaa gattggctgg 1501 cgaaaggatg cactgcattt gctggtgttc acaacagatg atgtgcccca catcgcattg 1561 gatggaaaat tgggaggcct ggtgcagcca cacgatggcc agtgccacct gaacgaggcc 1621 aacgagtaca ctgcatccaa ccagatggac tatccatccc ttgccttgct tggagagaaa 1681 ttggcagaga acaacatcaa cctcatcttt gcagtgacaa aaaaccatta tatgctgtac 1741 aagaatttta cagccctgat acctggaaca acggtggaga ttttagatgg agactccaaa 1801 aatattattc aactgattat taatgcatac aatagtatcc ggtctaaagt ggagttgtca 1861 gtctgggatc agcctgagga tcttaatctc ttctttactg ctacctgcca agatggggta 1921 tcctatcctg gtcagaggaa gtgtgagggt ctgaagattg gggacacggc atcttttgaa 1981 gtatcattgg aggcccgaag ctgtcccagc agacacacgg agcatgtgtt tgccctgcgg 2041 ccggtgggat tccgggacag cctggaggtg ggggtcacct acaactgcac gtgcggctgc 2101 agcgtggggc tggaacccaa cagcgccagg tgcaacggga gcgggaccta tgtctgcggc 2161 ctgtgtgagt gcagccccgg ctacctgggc accaggtgcg agtgccagga tggggagaac 2221 cagagcgtgt accagaacct gtgccgggag gcagagggca agccactgtg cagcgggcgt 2281 ggggactgca gctgcaacca gtgctcctgc ttcgagagcg agtttggcaa gatctatggg 2341 cctttctgtg agtgcgacaa cttctcctgt gccaggaaca agggagtcct ctgctcaggc 2401 catggcgagt gtcactgcgg ggaatgcaag tgccatgcag gttacatcgg ggacaactgt 2461 aactgctcga cagacatcag cacatgccgg ggcagagatg gccagatctg cagcgagcgt 2521 gggcactgtc tctgtgggca gtgccaatgc acggagccgg gggcctttgg ggagatgtgt 2581 gagaagtgcc ccacctgccc ggatgcatgc agcaccaaga gagattgcgt cgagtgcctg 2641 ctgctccact ctgggaaacc tgacaaccag acctgccaca gcctatgcag ggatgaggtg 2701 atcacatggg tggacaccat cgtgaaagat gaccaggagg ctgtgctatg tttctacaaa 2761 accgccaagg actgcgtcat gatgttcacc tatgtggagc tccccagtgg gaagtccaac 2821 ctgaccgtcc tcagggagcc agagtgtgga aacaccccca acgccatgac catcctcctg 2881 gctgtggtcg gtagcatcct ccttgttggg cttgcactcc tggctatctg gaagctgctt 2941 gtcaccatcc acgaccggag ggagtttgca aagtttcaga gcgagcgatc cagggcccgc 3001 tatgaaatgg cttcaaatcc attatacaga aagcctatct ccacgcacac tgtggacttc 3061 accttcaaca agttcaacaa atcctacaat ggcactgtgg acCTCGAGGA ACAAAAACTA 3121 ATATCAGAGG AAGATTTGGA ACAGAAACTA ATCAGTGAAG AAGATTTAGA GCAAAAGTTA 3181 ATTTCCGAGG AGGACTTATA GTGAGGGCCC GATATCTCGA CAATCAACCT CTGGATTACA 3241 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 3301 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 3361 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 3421 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 3481 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 3541 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 3601 TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA 3661 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 3721 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA 3781 GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAAACGG GGGAGGCTAA CTGAAACACG 3841 GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC 3901 GCACGGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG 3961 TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC 4021 CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC 4081 CTGCCATAGC AGATCTGCGC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG 4141 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 4201 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 4261 CCCGTCAAGC TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 4321 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 4381 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 4441 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA 4501 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT 4561 GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 4621 GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC 4681 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC 4741 TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT 4801 AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA 4861 GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC 4921 CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG 4981 ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA 5041 ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT 5101 TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG 5161 GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA 5221 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA 5281 CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT 5341 TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC 5401 TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC 5461 GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT 5521 GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT 5581 CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG 5641 TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT 5701 CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC 5761 GGGACTCTGG GGTTCGCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 5821 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 5881 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 5941 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 6001 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 6061 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 6121 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 6181 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 6241 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 6301 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 6361 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 6421 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 6481 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 6541 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 6601 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 6661 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC 6721 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 6781 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 6841 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 6901 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA 6961 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 7021 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 7081 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 7141 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 7201 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 7261 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 7321 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 7381 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 7441 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 7501 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 7561 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 7621 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 7681 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 7741 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 7801 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 7861 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 7921 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 7981 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 8041 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 8101 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 8161 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 8221 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC 8281 CGATCCCCTA TGGTCGACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA 8341 TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC 8401 AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.