pLV2-Shox2-promoter-EGFP-IRES-

pLV2-Shox2-promoter-EGFP-IRES-Puro plasmid

PVT97103

pLV2-Shox2-promoter-EGFP-IRES-Puro plasmid Information

Plasmid name: pLV2-Shox2(mouse)-promoter-EGFP-IRES-Puro
Alias: -
Gene lenght: 2215bp
Host: Mammalian cells,lentivirus
Use(s):
Promoter detection
Fragment type: Promoter
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: Shox2
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CPPT-F
Reverse primer:
Copy number: High
Induction:

pLV2-Shox2-promoter-EGFP-IRES-Puro plasmid Description

pLV2-Shox2-promoter-EGFP-IRES-Puro This is a plasmid suitable for promoter detection in mammalian cells, and can also be used for lentivirus packaging.

pLV2-Shox2-promoter-EGFP-IRES-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg gaattcgaag gataatgtca 2221 aagaaaaagt aaagcaacat ttctaataag ctttattttc atgtccttta aatgtgcaat 2281 gtagttagta ttacaagaag ttatggaacg tcttaaatta cacttaaatt ctcgactgag 2341 aatacattat gtaccacaat tctgtgttct ctttaccaat gttttaggtt gtttgggtag 2401 aacttctgaa attattggct aagtgtgttt tgttgttcta atcaataagt agaagatata 2461 catattaatg gcctgtaact gctttcattt ttactgagta ctggatccaa gagaacgaaa 2521 acattatctt ttaacatcaa acaaaactca ttaatattca gtatactgaa aatgcccccc 2581 caaactgcca cttaaagagg tatatttgtt tacatattca agtaaatgta acatttcata 2641 tgtaaacatt cttttgtgtg agcaaataag tatttgcata gctacaaatc acacagcaag 2701 ccgtggctta gaggagacag tcatgtcccc cccccccata taattaagag tttttataaa 2761 ataattgtca aatatacagt ttttaagtta taaatcagaa agagagttgt tttcccccct 2821 ccaaagagtg agtctttttt tcacagtcca cggtaaacaa aaattagtcg agcgacaagc 2881 aacatcggta attttttccg cagaactcaa aaagtagtca tcttttgttt ttcagaatta 2941 ggaaatcgaa ctagaatgcc ccccaagatg ctaaataatt aagggcaggg tgcagggagt 3001 tgttaggaaa aggagtaaga acccttagac ctcatagaga aaatcgaaat ggaactgcgc 3061 ctgaccgggc tgtaagaaaa taccttaaga ccgaatctgg cagagaacag ctgctcagga 3121 aacaagttaa atcgaccaac cccttctagc gggatgtgtc tttatgggag tcagctttga 3181 ctccaaagta gtagagtgaa ttacagggac cagtactcag tgaggctggc tgcgcgttcg 3241 gaagatccta aaaggaggtg aagccttccg aggtgctgca atgaagcccc attcgcagca 3301 ggcgcgtgaa gaccggatgc accgctgtgc agctgcagcg gcttggagca agaaagggtg 3361 caggaacgga atactggaat ggttgctagg cctattaacc atagagagcc cgatactggg 3421 ccagagacca ggggtcatca ttgtagggtc gagcagttgg gatgcccacc cagagcgagg 3481 ctgtagggtc gggtactctc tgaggatacc cactgactct gattgcaatg cacttgtcag 3541 tttcctcttg gccccgcctc cgagaagtct atccagttcc ccagtgtttt actaagtcgt 3601 gacaccaacg caaaatccac gcttaattaa attaattagg gattcttgtc aatcctggat 3661 taatggccaa gagcaccagc ggccgaggct atcagaaaac ccgaccgctg aggacctgcc 3721 tcctctcccc gccccctctg gtcccctgct tgccctccct ctccagcccg gtggactcca 3781 gcctggatcc tccatcctca atattcatat tcatattctc tctctctctc tctctctctc 3841 tctctctctc tctctctctc tctctctctc tctctctctc tctcgtcccc actccctctc 3901 ctccccaccc caggtagtgg cgctcctgct acccgcgaag gggttcacta ggagttgcga 3961 ggggaggtaa tttactcaga acaagaggcc cgcctttagg aagtataaat agtgagactt 4021 caagagctgc gtcgctatca gatctgaact ctccgcagga agaattgtca aagatcttaa 4081 cattgaacac gcgcactccg gcagggaagc atcagttccc atttccctct ggcggccccc 4141 gccccttccc tcctcccccc tctccccctt taccccctcc ttcctcctcc tcctcctcgt 4201 cctcctcctc ctcttacttc tttttctcct tctacttctc ctcttctttc ttctcctctt 4261 tttcttcttc ctcctcctcc ctctcctccc ccatccccct gccccattga tgtgttatta 4321 ttgggggggc tggagcagta aaaaaagaag gaggaaaaaa agagcggggc tcggcaggga 4381 gagcttgagc gcgaggttga ccggcggcgg cagcggccgc gctcgagGGC GGTGGAGGAA 4441 GCggcggagg tggatctatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 4501 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 4561 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 4621 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct 4681 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 4741 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 4801 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 4861 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg 4921 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg 4981 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 5041 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga 5101 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 5161 acgagctgta caagtagact agttctagag cggccgcgga tcccgcccct ctccctcccc 5221 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat 5281 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt 5341 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt 5401 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc 5461 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc 5521 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat 5581 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc 5641 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg 5701 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct 5761 ttgaaaaaca cgatgataag cttgccacaa cccacaagga gacgaccttc catgaccgag 5821 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc 5881 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc 5941 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 6001 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 6061 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 6121 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 6181 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 6241 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 6301 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 6361 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta gacgcgtctg 6421 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 6481 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 6541 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 6601 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 6661 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 6721 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 6781 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 6841 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 6901 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 6961 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 7021 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 7081 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 7141 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 7201 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt 7261 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg 7321 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca 7381 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg 7441 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca 7501 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct 7561 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg 7621 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct 7681 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 7741 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 7801 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 7861 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 7921 gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg 7981 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 8041 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 8101 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 8161 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 8221 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 8281 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 8341 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 8401 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 8461 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 8521 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 8581 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 8641 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 8701 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 8761 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 8821 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 8881 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 8941 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 9001 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 9061 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 9121 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 9181 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 9241 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 9301 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 9361 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 9421 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 9481 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 9541 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 9601 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 9661 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 9721 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 9781 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 9841 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 9901 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 9961 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 10021 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 10081 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga 10141 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 10201 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 10261 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc 10321 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct 10381 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta 10441 ctacaaactt agtagttttt aaagaaattg tatttgttaa atatgtacta caaacttagt 10501 agt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.