pLV2-Vsx2-promoter-EGFP-IRES-P

pLV2-Vsx2-promoter-EGFP-IRES-Puro plasmid

PVT96442

pLV2-Vsx2-promoter-EGFP-IRES-Puro plasmid Information

Plasmid name: pLV2-Vsx2(mouse)-promoter-EGFP-IRES-Puro
Alias: -
Gene lenght: 2116bp
Host: Mammalian cells,lentivirus
Use(s):
Promoter detection
Fragment type: Promoter
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: Vsx2
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CPPT-F
Reverse primer:
Copy number: High
Induction:

pLV2-Vsx2-promoter-EGFP-IRES-Puro plasmid Description

pLV2-Vsx2-promoter-EGFP-IRES-Puro This is a plasmid suitable for promoter detection in mammalian cells, and can also be used for lentivirus packaging.

pLV2-Vsx2-promoter-EGFP-IRES-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg gaattcCAGT TCCTGTTTTG 2221 ATCTTGTTTT GTTTTCGAGA ACAGGGTCTT AGGTAGTCCA GGCTGGCCTT GAACTCACTA 2281 TGTACCTACA GATGGTCTTG AACTCTGCTT CTCCAGCCTC TGCCTCCCAG GCGCTCAGAT 2341 TACAATGTGT GCTGCCAAAC CCAGCTCAAC AAAGCTTAAG TTCCTTCCTG GTGTCTCTTA 2401 CCTGTCAATC TGTCCCTGTA GAGGCCACCG AGAATACCAG ACACCATGGT AGCCAACCGA 2461 GGAGAGCTAG AAGGTTCCCC TTGCTTTGAA GGTTTGCAGC TGACAAGCCA AGATGCTCCA 2521 AATGAAATCT CCCGGCAGTC TCATTTCCCA TTCTGCTCAA AGTGACTAGC CCTGTCCTCA 2581 CCTTCTTCCT GAAGGGCAAA GAACCATCCA TTAAAACTCA TCTTTTCTGG TGATGGTGGC 2641 GCAGGCCTTT AATCCCAGCA CTAAGGAGGC AGAGGTAGGT GGATCTCTGT GAGTTCAAGT 2701 CCAGACTGGC CTATAGACTG AGTTTCAGAC AGCCAAAGCT ACACAGAGAC ACCCTGCCTC 2761 AAAAACAACA AAAACAAAAA CCCTAAACCA ACCAACCAAC AACAAAAACA AAAAACAAAA 2821 CAAAAGCAAT CCCCCTGCCT CATCATATGT TCTATATAGT GGATAGAGAA GCCCCCCAAT 2881 TAATAAAGTG ATCTCCAGTT CTGGGAGGGC AACAAGGAGC TAAACTGAAG GTCTAGAATG 2941 TGTATGGGAC ACATGTTCTC CTTGGCTCCT GACACTTAGA CCACACTCCA GTCTGAGATA 3001 TGAAGCAAGG ATATTTGAAA CACCAGTCCC CACGCCTCCA ACTCCAGGTG ACTCTCCCAG 3061 CCCGCCTGAG CAGGATGCTT CTCACCACAC TTCAGCTGTC CCCACTGAGT GCCCTTCTGA 3121 AGCCTCTGGA GAAGCAGGCA GTCAAGGGCC CCCAGCAGCT GGGAAAAGCG GCTCCTTTTT 3181 AGTTACTCCC CGCTCCCTTC TTCTGCATGG GGCGATCACA GCTTCACACC CTGCCTCTCT 3241 GGTTTACTGC AGAACCTCAG GCTTTTGGCT CGAAGCTGCG AGGTACTTGA TCCTTACTGA 3301 CTGTTGGACA GAATAAATAC AATCTTCGAG GGAAGCACTC CACGCTCATT CTCTGAGGAC 3361 GTTTAGAAAG ACAGCTGGAT AGTCAAGTCG GCCCCCAAAT GAAATCCCAG CCCTCGGGAG 3421 GACAGTACAG GAGAATGTTG AGTTTGAGAA CAACCCAGAC CATATAGAGG TCCTGTCTTA 3481 AACCACAAGG AAAAAAGAGG TGGCGTGGTG GGGGCGGGGT GGTGGTGGTG GAGTAAGGAG 3541 GACTGGATAA GGATAGGAGG GGAGGAAGGC CCCATATTTG TTGTCAACGA AAACCCTCTC 3601 TGTTTCTCTT TCCCACGAGG GCTCGATCCT AACCCTGATC CCTTTAACGT GGGCCAGTAT 3661 TTATTGAACA GCTCTTCTGA AATGAACATA ATGTCCCGTG CTGGGGAGAT AACTCTACCC 3721 ACACATCTCC CTCTGCACAG CTAAGCACCT TTCTGTATTC TCATGCCCAG CGCTCGGTCC 3781 AGGGCCCTCC AGCCTTCCTT GTCCCGTGTC TGAGTCTATT TGAGGCTGCT TAGTTAAGGG 3841 AGGGGGAGAG GTCTCCTAGC CTTTGCGTTC AGACCATCCC CACACCCCCA ACCCTACCCC 3901 AGGGATCTGT CGCAGCTAAC CTAGGCACTG GTCCCAGCCA GGTTATACAG CTCAAAACCG 3961 CTATCCACGG TATCTCCTGG AATAACCCCA AACCTGGCTG TTTCACCTCT GTCCCTGCAA 4021 GGGCACCCTG CAGAGCCCAC CACACTTGGG CCTGCTGCGC CCTGGCCCCT CGATACCGGT 4081 GGCGGCGGGA GCTGCCAGCT TTTTGGAATT CCTAATCGCT CCTGGCCCTG GTGATTGGCT 4141 GTTCAGCTCT GACCCCACTT GGGCTGCCGC CTGGTTGGAT AAAAAGGGAA TCTCCTTGGG 4201 GCTCCAGAGC ATTAGACACC GGAGGGGGCA CCTGGGACCA ACTTCACGAA GCGGGGAGCC 4261 CGGCGGGGGG GCTTGGGGGA GCAAAAGACC CGCGGCCTCA GCCCCTCCAA AGAACCGGGA 4321 GActcgagGG CGGTGGAGGA AGCggcggag gtggatctat ggtgagcaag ggcgaggagc 4381 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 4441 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 4501 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 4561 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 4621 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 4681 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 4741 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 4801 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 4861 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 4921 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 4981 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 5041 ccgggatcac tctcggcatg gacgagctgt acaagtagac tagttctaga gcggccgcgg 5101 atcccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg 5161 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag 5221 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc 5281 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg 5341 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag 5401 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca 5461 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt 5521 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc 5581 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa 5641 ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca acccacaagg 5701 agacgacctt ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 5761 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 5821 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 5881 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 5941 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 6001 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 6061 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 6121 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 6181 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 6241 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 6301 cccggtgcct agacgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt 6361 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 6421 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 6481 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 6541 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 6601 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 6661 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 6721 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 6781 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 6841 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg 6901 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc 6961 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag 7021 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca 7081 gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc 7141 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat 7201 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac 7261 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc 7321 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg 7381 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc 7441 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg 7501 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct 7561 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 7621 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 7681 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 7741 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 7801 gaaatgaata tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag 7861 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 7921 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 7981 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 8041 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 8101 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 8161 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 8221 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 8281 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 8341 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 8401 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 8461 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 8521 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 8581 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 8641 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 8701 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 8761 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 8821 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 8881 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 8941 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 9001 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 9061 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 9121 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 9181 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 9241 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 9301 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 9361 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 9421 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 9481 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 9541 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 9601 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 9661 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 9721 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 9781 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 9841 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 9901 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 9961 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 10021 aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg 10081 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 10141 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa 10201 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt 10261 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa 10321 attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt gtatttgtta 10381 aatatgtact acaaacttag tagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.