pLV3-CMV-WDR43-3×FLAG-CopGFP-P

pLV3-CMV-WDR43-3×FLAG-CopGFP-Puro plasmid

PVT97101

pLV3-CMV-WDR43-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-WDR43(human)-3×FLAG-CopGFP-Puro
Alias: NM_015131.3;NET12; UTP5
Gene lenght: 2034bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-WDR43-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-WDR43-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-WDR43-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg cggcgggcgg cggcggtagc 2341 tgcgaccccc tggcccctgc tggggtccct tgcgccttct ccccgcacag ccaggcctac 2401 ttcgctttgg cctctaccga cggtcactta cgagtatggg agacggccaa caaccggctg 2461 caccaggagt acgtgccttc cgcgcacctc agtggtacct gcacctgtct ggcctgggcg 2521 ccagcgcggc tgcaggccaa ggaaagtccc cagaggaaaa aaaggaaatc agaagctgta 2581 ggaatgagta accagactga cttattggct cttggcacag cagttggtag cattttatta 2641 tacagcacag taaaaggaga gttacacagt aaattaataa gtggtggaca tgacaacaga 2701 gtcaactgca tacagtggca tcaagacagt ggctgtttat atagttgttc agatgataaa 2761 catattgtgg aatggaacgt acagacatgc aaagtaaagt gcaaatggaa aggcgacaat 2821 agcagtgtca gttccctatg tatcagccca gatggaaaga tgttgctttc agctggtcga 2881 acaatcaaac tatgggtttt ggagaccaaa gaagtctaca ggcatttcac aggacatgca 2941 acgccagttt cgtcactgat gttcactacc atcagacctc ctaatgagag ccagcccttt 3001 gatggaatta caggtcttta tttcttatct ggagcagtac atgaccggtt acttaatgtc 3061 tggcaggtcc gatcagaaaa caaagaaaag agtgcagtga tgtcatttac agttaccgat 3121 gaacctgtct atattgactt aactttgtca gaaaacaaag aagagcctgt caagttggct 3181 gttgtttgca gagatggtca agtccatctt tttgaacaca tattaaatgg gtactgcaaa 3241 aagcctttga cttcaaactg cacaattcag atagcaacac ctgggaaagg caagaagtca 3301 acaccaaaac ccatccctat tctagctgct ggtttttgct cagacaaaat gtcattgttg 3361 cttgtatatg gcagttggtt tcagcctact attgagcgag tggctttaaa ctccagagaa 3421 cctcatatgt gtttagtaag agatatttca aactgctggg cccccaaagt agaaacagct 3481 ataacaaagg tgaggacacc agtgatgaat tctgaagcaa aagttctggt gcctgggatt 3541 cctggtcatc atgcagctat caagcccgct cctccacaaa ccgagcaagt agagagcaag 3601 aggaagtcag ggggaaatga ggttagcatt gaagaacgtc tgggagcaat ggatatagac 3661 acacacaaaa aaggaaagga agacctccag acgaatagct ttccagttct tcttacccag 3721 ggcttagaaa gtaacgattt tgaaatgcta aataaagtac ttcaaactag gaatgtaaac 3781 cttataaaga agactgtatt aaggatgccc ctgcatacta ttattccgtt gttacaagag 3841 cttacaaaga ggttacaagg acatcctaat agtgctgtgc taatggttca gtggctaaaa 3901 tgtgtgttaa cagttcatgc atcatacctg tccacgttgc ctgacctggt accccagctg 3961 gggacactct accagttaat ggaaagcaga gtcaaaactt ttcagaaact ttcacacctt 4021 catggaaagc ttattcttct aattacacaa gtaacagcat cagagaagac aaagggagca 4081 acttcccctg gacagaaggc aaagttggtg tatgaagaag agtcttctga agaggagtct 4141 gatgatgaaa tagcagataa ggattctgaa gataattggg atgaagatga ggaggagagt 4201 gaaagtgaaa aagatgagga cgttgaagag gaagatgagg atgccgaagg aaaagatgaa 4261 gaaaatggcg aggacagaga tacagcaagt gaaaaagaat taaatggaga ttctgattta 4321 gatcctgaaa atgaaagtga agaagaagga tccGACTACA AAGACCATGA CGGTGATTAT 4381 AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGt agcggccgcG aaggatctgc 4441 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 4501 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa 4561 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 4621 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag 4681 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca 4741 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc 4801 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc 4861 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc 4921 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta 4981 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc 5041 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag 5101 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac 5161 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac 5221 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag 5281 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg 5341 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac 5401 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg 5461 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg 5521 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc 5581 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag 5641 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat 5701 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt 5761 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg 5821 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 5881 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 5941 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 6001 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 6061 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 6121 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 6181 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 6241 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 6301 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 6361 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa 6421 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 6481 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 6541 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 6601 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 6661 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 6721 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 6781 gtgttgtcgg ggaaATCATC GTCCTTTCCT tggctgctcg cctgtgttgc cacctggatt 6841 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 6901 cgcggcctgc tgccggctct gcggcctctt ccgcgtctTc gccttcgccc tcagacgagt 6961 cggatctccc tttggGccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc 7021 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 7081 ccaacgaaGa taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 7141 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 7201 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 7261 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 7321 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 7381 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 7441 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct 7501 ctagctaTCC CGCCCCTAAC TCCGCCCAtc ccgcccctaa ctccgcccag ttccgcccat 7561 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 7621 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac 7681 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 7741 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 7801 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 7861 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 7921 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 7981 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 8041 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 8101 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 8161 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 8221 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 8281 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 8341 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 8401 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 8461 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 8521 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 8581 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 8641 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 8701 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 8761 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 8821 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 8881 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 8941 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 9001 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 9061 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 9121 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 9181 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 9241 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 9301 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 9361 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 9421 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 9481 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 9541 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 9601 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 9661 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 9721 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 9781 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 9841 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 9901 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 9961 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 10021 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 10081 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 10141 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 10201 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 10261 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 10321 g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.