pMA5-HpaII-mCherry(opt) plasmi

pMA5-HpaII-mCherry(opt) plasmid

PVT97002

pMA5-HpaII-mCherry(opt) plasmid Information

Plasmid name: pMA5-HpaII-mCherry(opt)
Alias: -
Gene lenght:
Host: Bacillus subtilis
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Kan
Fluorescent labeling: Red
Promoter: HpaII
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: HpaII-F
Reverse primer:
Copy number: High
Induction:

pMA5-HpaII-mCherry(opt) plasmid Description

pMA5-HpaII-mCherry(opt) A plasmid suitable for protein expression in Bacillus subtilis.

pMA5-HpaII-mCherry(opt) plasmid Sequence

1 catatgagtt atgcagtttg tagaatgcaa aaagtgaaat cagggggatc cATGGTCAGC 61 AAAGGAGAAG AAGATAACAT GGCAATCATC AAAGAATTTA TGCGGTTTAA AGTTCATATG 121 GAAGGCAGCG TGAATGGCCA TGAATTTGAA ATTGAAGGCG AAGGCGAAGG AAGACCATAT 181 GAAGGCACAC AGACAGCTAA ACTGAAAGTG ACAAAAGGCG GACCGCTGCC GTTTGCATGG 241 GATATTCTTA GCCCGCAGTT TATGTATGGA AGCAAAGCGT ATGTCAAACA TCCGGCGGAT 301 ATCCCGGATT ATCTGAAACT GAGCTTTCCT GAAGGCTTTA AATGGGAAAG AGTCATGAAC 361 TTTGAAGATG GCGGAGTTGT TACAGTTACA CAGGACTCAT CATTACAAGA TGGCGAATTT 421 ATTTATAAAG TAAAACTGCG CGGAACAAAT TTTCCGAGCG ATGGCCCGGT GATGCAAAAA 481 AAAACGATGG GCTGGGAAGC TTCTTCAGAA AGAATGTATC CGGAAGATGG AGCCCTGAAA 541 GGAGAAATTA AACAACGCTT AAAACTGAAA GATGGCGGCC ATTATGATGC AGAAGTGAAA 601 ACGACATATA AAGCGAAAAA ACCGGTCCAA TTACCTGGAG CGTATAATGT CAACATCAAA 661 TTAGATATTA CATCACATAA CGAAGATTAT ACAATTGTGG AACAGTATGA ACGCGCAGAA 721 GGTAGACATA GCACCGGGGG TATGGATGAA CTGTATAAAT AGgctagctt ggtacgtacc 781 agatctgaga tcacgcgttc tagaggtcga aattcacctc gaaagcaagc tgataaaccg 841 atacaattaa aggctccttt tggagccttt ttttttggag attttcaacg tgaaaaaatt 901 attattcgca attccaagct aattcacctc gaaagcaagc tgataaaccg atacaattaa 961 aggctccttt tggagccttt ttttttggag attttcaacg tgaaaaaatt attattcgca 1021 attccaagct ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 1081 tcccggagac ggtcacagct tgtctgtaag cggatgcaga tcacgcgccc tgtagcggcg 1141 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 1201 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 1261 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 1321 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 1381 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 1441 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 1501 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 1561 tattaacgct tacaatttGA TCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT 1621 CACTCAAAGC GGTAATACGG TTATCCCAGA ATCCGGGGAT AACGCAGGAA AGACaggtgg 1681 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 1741 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 1801 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 1861 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 1921 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 1981 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 2041 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 2101 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 2161 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 2221 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 2281 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 2341 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 2401 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 2461 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 2521 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 2581 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 2641 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 2701 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 2761 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 2821 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 2881 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 2941 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 3001 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 3061 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 3121 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 3181 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 3241 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 3301 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 3361 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 3421 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 3481 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 3541 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 3601 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 3661 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 3721 ttagctcact cattaggcac cccaggcttt acactttatg cttccggcat attctcaata 3781 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg 3841 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt 3901 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct 3961 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag 4021 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc 4081 taaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta 4141 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct 4201 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca 4261 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg 4321 gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct 4381 tccgtcacag gtatttattc gaagacgaaa gggcatcgcg cgcggggaat tcccgggaga 4441 gctcgatatc gcatgcggta cctctagaag aagcttggag acaaggtaaa ggataaaaca 4501 gcacaattcc aagaaaaaca cgatttagaa cctaaaaaga acgaatttga actaactcat 4561 aaccgagagg taaaaaaaga acgaagtcga gatcagggaa tgagtttata aaataaaaaa 4621 agcacctgaa aaggtgtctt tttttgatgg ttttgaactt gttctttctt atcttgatac 4681 atatagaaat aacgtcattt ttattttagt tgctgaaagg tgcgttgaag tgttggtatg 4741 tatgtgtttt aaagtattga aaacccttaa aattggttgc acagaaaaac cccatctgtt 4801 aaagttataa gtgactaaac aaataactaa atagatgggg gtttctttta atattatgtg 4861 tcctaatagt agcatttatt cagatgaaaa atcaagggtt ttagtggaca agacaaaaag 4921 tggaaaagtg agaccatgga gagaaaagaa aatcgctaat gttgattact ttgaacttct 4981 gcatattctt gaatttaaaa aggctgaaag agtaaaagat tgtgctgaaa tattagagta 5041 taaacaaaat cgtgaaacag gcgaaagaaa gttgtatcga gtgtggtttt gtaaatccag 5101 gctttgtcca atgtgcaact ggaggagagc aatgaaacat ggcattcagt cacaaaaggt 5161 tgttgctgaa gttattaaac aaaagccaac agttcgttgg ttgtttctca cattaacagt 5221 taaaaatgtt tatgatggcg aagaattaaa taagagtttg tcagatatgg ctcaaggatt 5281 tcgccgaatg atgcaatata aaaaaattaa taaaaatctt gttggtttta tgcgtgcaac 5341 ggaagtgaca ataaataata aagataattc ttataatcag cacatgcatg tattggtatg 5401 tgtggaacca acttatttta agaatacaga aaactacgtg aatcaaaaac aatggattca 5461 attttggaaa aaggcaatga aattagacta tgatccaaat gtaaaagttc aaatgattcg 5521 accgaaaaat aaatataaat cggatataca atcggcaatt gacgaaactg caaaatatcc 5581 tgtaaaggat acggatttta tgaccgatga tgaagaaaag aatttgaaac gtttgtctga 5641 tttggaggaa ggtttacacc gtaaaaggtt aatctcctat ggtggtttgt taaaagaaat 5701 acataaaaaa ttaaaccttg atgacacaga agaaggcgat ttgattcata cagatgatga 5761 cgaaaaagcc gatgaagatg gattttctat tattgcaatg tggaattggg aacggaaaaa 5821 ttattttatt aaagagtagt tcaacaaacg ggccagtttg ttgaagatta gatgctataa 5881 ttgttattaa aaggattgaa ggatgcttag gaagacgagt tattaatagc tgaataagaa 5941 cggtgctctc caaatattct tatttagaaa agcaaatcta aaattatctg aaaagggaat 6001 gagaatagtg aatggaccaa taataatgac tagagaagaa agaatgaaga ttgttcatga 6061 aattaaggaa cgaatattgg ataaatatgg ggatgatgtt aaggctattg gtgtttatgg 6121 ctctcttggt cgtcagactg atgggcccta ttcggatatt gagatgatgt gtgtcatgtc 6181 aacagaggaa gcagagttca gccatgaatg gacaaccggt gagtggaagg tggaagtgaa 6241 ttttgatagc gaagagattc tactagatta tgcatctcag gtggaatcag attggccgct 6301 tacacatggt caatttttct ctattttgcc gatttatgat tcaggtggat acttagagaa 6361 agtgtatcaa actgctaaat cggtagaagc ccaaacgttc cacgatgcga tttgtgccct 6421 tatcgtagaa gagctgtttg aatatgcagg caaatggcgt aatattcgtg tgcaaggacc 6481 gacaacattt ctaccatcct tgactgtaca ggtagcaatg gcaggtgcca tgttgattgg 6541 tctgcatcat cgcatctgtt atacgacgag cgcttcggtc ttaactgaag cagttaagca 6601 atcagatctt ccttcaggtt atgaccatct gtgccagttc gtaatgtctg gtcaactttc 6661 cgactctgag aaacttctgg aatcgctaga gaatttctgg aatgggattc aggagtggac 6721 agaacgacac ggatatatag tggatgtgtc aaaacgcata ccattttgaa cgatgacctc 6781 taataattgt taatcatgtt ggttacgtat ttattaactt ctcctagtat tagtaattat 6841 catggctgtc atggcgcatt aacggaataa agggtgtgct taaatcgggc cattttgcgt 6901 aataagaaaa aggattaatt atgagcgaat tgaattaata ataaggtaat agatttacat 6961 tagaaaatga aaggggattt tatgcgtgag aatgttacag tctatcccgg cattgccagt 7021 cggggatatt aaaaagagta taggttttta ttgcgataaa ctaggtttca ctttggttca 7081 ccatgaagat ggattcgcag ttctaatgtg taatgaggtt cggattcatc tatgggaggc 7141 aagtgatgaa ggctggcgct ctcgtagtaa tgattcaccg gtttgtacag gtgcggagtc 7201 gtttattgct ggtactgcta gttgccgcat tgaagtagag ggaattgatg aattatatca 7261 acatattaag cctttgggca ttttgcaccc caatacatca ttaaaagatc agtggtggga 7321 tgaacgagac tttgcagtaa ttgatcccga caacaatttg attagctttt ttcaacaaat 7381 aaaaagctaa aatctattat taatctgttc agcaatcggg cgcgattgct gaataaaaga 7441 tacgagagac ctctcttgta tcttttttat tttgagtggt tttgtccgtt acactagaaa 7501 accgaaagac aataaaaatt ttattcttgc tgagtctggc tttcggtaag ctagacaaaa 7561 cggacaaaat aaaaattggc aagggtttaa aggtggagat tttttgagtg atcttctcaa 7621 aaaatactac ctgtcccttg ctgattttta aacgagcacg agagcaaaac ccccctttgc 7681 tgaggtggca gagggcaggt ttttttgttt cttttttctc gtaaaaaaaa gaaaggtctt 7741 aaaggtttta tggttttggt cggcactgcc gacagcctcg cagagcacac actttatgaa 7801 tataaagtat agtgtgttat actttacttg gaagtggttg ccggaaagag cgaaaatgcc 7861 tcacatttgt gccacctaaa aaggagcgat tta

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.