pNIBAN1-Fluc plasmid

pNIBAN1-Fluc plasmid

PVT96993

pNIBAN1-Fluc plasmid Information

Plasmid name: pNIBAN1(human)-Fluc
Alias: -
Gene lenght: 1200bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: NIBAN1
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: RVP3
Reverse primer:
Copy number: High
Induction:

pNIBAN1-Fluc plasmid Description

pNIBAN1-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pNIBAN1-Fluc plasmid Sequence

1 ggtaccTGAA TAAGGTGAGG AATGATATGG GATGTCAGTA GAGGGGATGA GAATTGGTTG 61 GCTATTGGAC ATATTTAAAA GCTGAATTGA CAGGATTTAC TGACAGATTG GAATGTAGAG 121 TGTGAGAGAT TGGGTGTGAG TGTGATGAAA TAATTGTGTT AAAGTGCAAG TTAGCTAATA 181 GGGAAGGCGG GGGTGTGTGG GAAAGCCTTC AAAGTCCTCC TCAGGCCTGG TTCTAGTGTA 241 TACAGACCAT TTGACCTTGA GGTTAAGTCA ATAGGAGTCG CAAAATAACT GACTACTAAG 301 AATAGGATGA AATCAGCGGT GACCCCTCTG CCCAAGTGGG TCTTGTTCCG AGAGGCTGCC 361 AAGCTAAATA GTGGTTGTGG GAAAAGAACA AATATCTTTT CTAATGATTG ATTTCCAGTT 421 AAGGAGAGGA ATGGAAAGTA AAGGGGTAAG GACAGACGTT CATCAAGTCT TAGGAAGTTT 481 CAGGGCTGGG AGTATATTGC AGGACCAAGC CTTAGTTTTT CCCTGTCTTT CCATTTTTTT 541 TTCTCTTTCC TTTTTTCCTC ATTTCCCCAC TCCCTCCTTG GTCCAGGTTC TCGCCTGCAG 601 AAAGGTATAG TTTACCTCTA GTTCTCAAAC TTCAGCGGGC ATCAGAATTC CCTGGAGGGT 661 TTGTGCAAAC ACAGATTGCT GGGCTCCACT CCCACAGATT CTAAGTGTGT GTGTGTCTGT 721 GTGTGTGTAG AGGGGTGGTG GACGAAAGCG CTGAGATTTT GTATTTCTAA CAGGCTCCCA 781 GGTAATGCTT GGGAAGAACT GTTGCTTCTT GGACAGCAAC ACTTACAAGG CAACGTGATC 841 TTGAGCTCCA CCTCGAAGGA TGTGAGCTGG GAAAGTTCCA GCTCACAGAG ATTTAGTCAC 901 TTGAGAAATG GTCCACTTCC TCCAAGGTGT TTCTTGGATG CCCCAAAGGG ATGAAAACAA 961 GAAGTGACCG TGGGCGAGGA GGAGGAGCTG GCCGACCTCC TCGGTTGCCA GCCCGTCGGA 1021 GCTCACCAGC TAGGCGAGGG CGGGAAGACA GCCCCCGGGC CGGCCCACGG ATCATCAGAA 1081 GGCGCGGACC TGGAGGAGGC GCCCCAGAAG GCGACGCCTC TTGCCTCCTG TCTCTCGCCT 1141 CTCGAAGGAA GTTTGCTCTT AATTTCAGAG CCGGGTTCGC CGTCGGATCA ACCTCCAGGA 1201 GCTAGCaagc ttggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca 1261 taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc 1321 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata 1381 tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta 1441 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc 1501 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg 1561 acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt 1621 tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc 1681 aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt 1741 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg 1801 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag 1861 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt 1921 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt 1981 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata 2041 gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc 2101 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat 2161 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg 2221 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat 2281 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc 2341 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa 2401 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag 2461 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg 2521 acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct 2581 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg 2641 caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt 2701 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa 2761 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta 2821 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa 2881 agatcgccgt gtaattctag agtcggggcg gccggccgct tcgagcagac atgataagat 2941 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg 3001 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca 3061 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa 3121 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg 3181 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca 3241 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc 3301 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 3361 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 3421 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 3481 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 3541 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 3601 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 3661 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 3721 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 3781 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 3841 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 3901 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 3961 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 4021 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 4081 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 4141 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 4201 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 4261 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 4321 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 4381 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 4441 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 4501 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 4561 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 4621 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 4681 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 4741 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 4801 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 4861 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 4921 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 4981 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 5041 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 5101 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 5161 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 5221 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 5281 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 5341 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 5401 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 5461 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 5521 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 5581 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 5641 atttaacgcg aattttaaca aaatattaac gtttacaatt tcccattcgc cattcaggct 5701 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct 5761 accatgataa gtaagtaata ttaaggtacg ggaggtactt ggagcggccg caataaaata 5821 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac 5881 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca 5941 agtgcaggtg ccagaacatt tctctatcga ta

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.