pLV3-CMV-MED18-3×FLAG-CopGFP-P

pLV3-CMV-MED18-3×FLAG-CopGFP-Puro plasmid

PVT88222

pLV3-CMV-MED18-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-MED18(human)-3×FLAG-CopGFP-Puro
Alias: NM_017638.3;p28b; SRB5
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MED18-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-MED18-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MED18-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg aggcacctcc agtcaccatg 2341 atgcctgtca ctgggggcac cattaacatg atggagtacc tgttgcaggg aagtgtttta 2401 gatcacagtt tggaaagcct catccaccgc cttcgtggtt tgtgtgacaa catggaacct 2461 gagactttcc ttgaccatga gatggtattc ctccttaagg gccagcaagc cagcccattt 2521 gttctcaggg cccgacgctc tatggacagg gcaggggcac cctggcatct gcgctacctg 2581 ggacagccag aaatgggaga caagaaccgc catgccctgg tgcgaaactg cgtggacatt 2641 gccacatctg agaacctcac cgacttcttg atggaaatgg gcttccgcat ggaccatgag 2701 tttgttgcta agggacattt gttccgtaag ggcatcatga agattatggt gtacaagatt 2761 ttccgcatcc tggtgccagg gaacacagac agcactgagg ccttgtcact ctcctatctc 2821 gtggaattaa gtgtggtagc acccgctggg caggacatgg tctctgatga catgaagaac 2881 ttcgcagaac agctaaaacc tctggttcac ctagagaaaa tagaccccaa gaggctcatg 2941 ggatccGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 3001 GACGATGACA AGtagcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca 3061 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 3121 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 3181 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 3241 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 3301 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 3361 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 3421 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 3481 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 3541 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc 3601 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc 3661 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag 3721 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc 3781 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac 3841 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg 3901 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc 3961 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg 4021 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc 4081 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc 4141 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag 4201 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc 4261 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc 4321 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag 4381 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 4441 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 4501 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 4561 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 4621 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 4681 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 4741 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 4801 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 4861 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 4921 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 4981 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 5041 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 5101 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 5161 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 5221 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 5281 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 5341 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaATC ATCGTCCTTT 5401 CCTtggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 5461 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 5521 cttccgcgtc tTcgccttcg ccctcagacg agtcggatct ccctttggGc cgcctccccg 5581 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 5641 agaaaagggg ggactggaag ggctaattca ctcccaacga aGataagatc tgctttttgc 5701 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 5761 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 5821 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 5881 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 5941 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 6001 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 6061 caaactcatc aatgtatctt atcatgtctg gctctagcta TCCCGCCCCT AACTCCGCCC 6121 Atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 6181 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 6241 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt 6301 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 6361 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 6421 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 6481 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 6541 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 6601 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 6661 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 6721 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 6781 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 6841 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 6901 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 6961 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 7021 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 7081 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 7141 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7201 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 7261 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 7321 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 7381 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 7441 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 7501 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 7561 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 7621 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 7681 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 7741 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 7801 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 7861 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 7921 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 7981 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 8041 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 8101 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 8161 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 8221 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 8281 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 8341 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 8401 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 8461 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 8521 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 8581 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 8641 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 8701 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 8761 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 8821 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 8881 acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.