pLV3-CMV-DTX4-3×FLAG-CopGFP-Pu

pLV3-CMV-DTX4-3×FLAG-CopGFP-Puro plasmid

PVT96956

pLV3-CMV-DTX4-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-DTX4(human)-3×FLAG-CopGFP-Puro
Alias: NM_015177.2;RNF155
Gene lenght: 1857bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-DTX4-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-DTX4-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-DTX4-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc tcctggcctc ggccgtggtg 2341 gtctgggaat ggctgaacga gcacggccgc tggcgtccct acagcccagc ggtgagccac 2401 cacatcgagg cggtggtccg cgccggcccc cgcgcggggg gcagcgtggt gctgggccag 2461 gtggacagcc gtctcgcgcc ctacatcatc gacctgcagt ccatgaacca gttccgccaa 2521 gacacgggaa ctctccgccc agttcgccgc aactactacg acccctcctc ggcccctggg 2581 aagggcgtgg tgtgggagtg ggagaacgac aatggctcct ggacgcccta cgacatggaa 2641 gtgggcatca ccatccagca tgcctatgag aagcagcacc cctggatcga cctcacttcc 2701 attggcttta gctacgtaat tgacttcaac accatgggcc agatcaaccg tcagacccag 2761 cgccaacgcc gcgtccgccg gcgcctcgac ctcatctacc ccatggtcac agggaccttg 2821 cctaaggctc agtcctggcc agtcagccct gggccagcca cctcgccccc catgtccccc 2881 tgctcctgtc cccagtgtgt cttggtgatg agtgttaagg cagccgtggt caatggcagc 2941 actgggcccc tacagctgcc agtgacccgc aagaacatgc cgcctcctgg agtggtcaag 3001 ctacccccac tgccaggctc tggggccaag ccactggaca gcacaggcac cattcgaggc 3061 ccactgaaga ccgccccatc gcaggtgatc cggagacaag cctccagcat gcccactggg 3121 acaaccatgg gctctcctgc cagtccccca ggacccaaca gcaagaccgg aagggtggcc 3181 ctggccacct tgaatcgtac caacctgcag cgactggcca ttgcccagtc ccgggtgctg 3241 atcgcctctg gggtccccac agtcccagtg aagaacctaa atgggtccag tcctgtcaac 3301 cctgccttgg caggaatcac tgggatcctc atgagtgcag cggggctgcc tgtgtgtctc 3361 accaggccac caaagctggt cctacaccca ccccccgtca gcaagagtga aataaaatcc 3421 atcccagggg tttccaacac aagccgcaag accaccaaaa aacaagccaa gaaaggtaaa 3481 accccagagg aagtgctaaa aaaatatcta cagaaagtcc ggcacccacc agatgaggac 3541 tgcaccatct gtatggaacg cctcacggcc ccctcaggct acaagggccc gcagcctacg 3601 gtaaaacctg acctggtagg gaagctgtcc agatgcggcc acgtctacca catctactgc 3661 ttggttgcca tgtacaacaa tgggaacaag gatggaagtt tgcagtgtcc aacctgcaag 3721 accatttatg gggtgaagac aggcacccaa cctccaggga agatggagta ccacctcatc 3781 ccccactcct tgcctggcca cccagactgc aaaaccatcc ggatcatcta cagcatcccc 3841 cccggcattc agggaccgga acacccgaat cctgggaaga gtttcagcgc ccgaggcttc 3901 ccacgacact gttaccttcc ggacagcgag aaagggagaa aagttctgaa gctgctgctc 3961 gtggcctggg atcgccgcct catttttgcc attggcacct ccagcaccac aggcgagtca 4021 gacaccgtca tctggaatga ggtccaccac aagacagagt ttggctctaa tctcactggc 4081 catggctacc cagatgccaa ttacctggat aatgtgctgg ctgaactggc tgcccagggc 4141 atctctgagg acagcactgc ccaggagaag gacggatccG ACTACAAAGA CCATGACGGT 4201 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGtagcg gccgcGaagg 4261 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 4321 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 4381 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 4441 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 4501 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 4561 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 4621 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 4681 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 4741 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 4801 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 4861 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 4921 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 4981 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 5041 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 5101 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 5161 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 5221 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 5281 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 5341 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 5401 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 5461 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 5521 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 5581 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag 5641 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 5701 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 5761 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 5821 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 5881 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 5941 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 6001 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 6061 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 6121 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 6181 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat 6241 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 6301 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 6361 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 6421 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 6481 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 6541 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 6601 tccgtggtgt tgtcggggaa ATCATCGTCC TTTCCTtggc tgctcgcctg tgttgccacc 6661 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 6721 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctTcgcct tcgccctcag 6781 acgagtcgga tctccctttg gGccgcctcc ccgcctggta cctttaagac caatgactta 6841 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 6901 tcactcccaa cgaaGataag atctgctttt tgcttgtact gggtctctct ggttagacca 6961 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 7021 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 7081 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 7141 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 7201 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 7261 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 7321 ctggctctag ctaTCCCGCC CCTAACTCCG CCCAtcccgc ccctaactcc gcccagttcc 7381 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 7441 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 7501 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 7561 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 7621 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 7681 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 7741 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 7801 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 7861 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 7921 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 7981 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 8041 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 8101 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 8161 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 8221 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 8281 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 8341 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 8401 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 8461 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 8521 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 8581 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 8641 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 8701 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 8761 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 8821 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 8881 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 8941 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 9001 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 9061 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 9121 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 9181 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 9241 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 9301 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 9361 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 9421 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 9481 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 9541 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 9601 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 9661 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 9721 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 9781 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 9841 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 9901 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 9961 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 10021 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 10081 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 10141 caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.