pLV3-U6-TBX1-shRNA3-Blast plas

pLV3-U6-TBX1-shRNA3-Blast plasmid

PVT89342

pLV3-U6-TBX1-shRNA3-Blast plasmid Information

Plasmid name: pLV3-U6-TBX1(human)-shRNA3-Blast
Alias: TGGATCCCATGGCCGACTATA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-TBX1-shRNA3-Blast plasmid Description

pLV3-U6-TBX1-shRNA3-Blast This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-TBX1-shRNA3-Blast plasmid Sequence

1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccGC CACCATGGCC 541 AAGCCTTTGT CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC 601 ATCCCCATCT CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC 661 TTCACTGGTG TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG 721 GGCACTGCTG CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG 781 AACAGGGGCA TCTTGAGCCC CTGCGGACGG TGCCGACAGG TGCTTCTCGA TCTGCATCCT 841 GGGATCAAAG CCATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA 901 TTGCTGCCCT CTGGTTATGT GTGGGAGGGC TAAGTCGACg gtacctttaa gaccaatgac 961 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 1021 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga 1081 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 1141 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 1201 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 1261 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 1321 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 1381 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 1441 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 1501 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 1561 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg 1621 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac 1681 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 1741 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 1801 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 1861 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 1921 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 1981 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 2041 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 2101 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 2161 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 2221 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg 2281 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 2341 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 2401 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 2461 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 2521 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 2581 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 2641 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 2701 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 2761 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 2821 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 2881 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 2941 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 3001 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 3061 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 3121 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 3181 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 3241 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 3301 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 3361 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 3421 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 3481 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 3541 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 3601 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 3661 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 3721 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 3781 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 3841 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 3901 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 3961 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 4021 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 4081 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 4141 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 4201 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 4261 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 4321 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 4381 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 4441 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct 4501 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa 4561 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta 4621 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc 4681 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag 4741 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 4801 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 4861 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact 4921 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc 4981 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag 5041 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc 5101 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag 5161 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag 5221 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac 5281 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa 5341 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg 5401 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga 5461 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag 5521 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt 5581 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag 5641 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag 5701 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc 5761 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga 5821 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa 5881 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc 5941 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta 6001 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg 6061 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct 6121 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac 6181 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga 6241 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatc acgagactag 6301 cctcgagcgg ccgccccctt caccgagggc ctatttccca tgattccttc atatttgcat 6361 atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata 6421 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa 6481 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg 6541 gctttatata tcttgtggaa aggacgaaac ACCGGTGGAT CCCATGGCCG ACTATActcg 6601 agTATAGTCG GCCATGGGAT CCAttttttG AATTCtcgac ctcgagacaa atggcagtat 6661 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag 6721 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc 6781 aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.