pLV3-EF1a-Slc25a3-PGK-CopGFP-P

pLV3-EF1a-Slc25a3-PGK-CopGFP-Puro plasmid

PVT96881

pLV3-EF1a-Slc25a3-PGK-CopGFP-Puro plasmid Information

Plasmid name: pLV3-EF1a-Slc25a3(mouse)-PGK-CopGFP-Puro
Alias: NM_133668.5;5730556H19Rik; Phc; PTP;
Gene lenght: 1074bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F-72
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-Slc25a3-PGK-CopGFP-Puro plasmid Description

pLV3-EF1a-Slc25a3-PGK-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-Slc25a3-PGK-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cGCCACCatg ttctcgtccg tagcgcacct 2521 ggcgcgggcg aaccccttca acgcgccaca cctgcagctg gtgcacgatg gcctgtcggg 2581 tccccgcagt cccccagctc cgccccggcg ttcccgccac ctggccgccg ccgccgtgga 2641 agagtacagt tgtgaatttg gctccatgaa gtattatgca ctgtgtggct ttggtggggt 2701 cttaagttgt gggctgacac acactgctgt tgttcccctg gacttagtaa agtgccgcat 2761 gcaggtggac cctcagaagt acaaaggcat atttaatgga ttctccatta cactgaaaga 2821 agatggcgtt cgtggtttgg ctaaaggatg ggccccaact ttgattggct attccatgca 2881 agggctctgc aaattcggct tttatgaagt cttcaaagcc ttatatagca acatacttgg 2941 tgaggaaaac acctacctgt ggcgcacatc actgtattta gcttcttctg ccagtgctga 3001 attcttcgct gacattgccc tggctcctat ggaagctgct aaagttcgaa ttcaaaccca 3061 gcctggttat gccaacacct tgagggaagc tgttcccaaa atgtataaag aggaaggctt 3121 aaatgcgttc tacaagggcg ttgctcctct gtggatgaga cagatcccgt acaccatgat 3181 gaagttcgcc tgctttgaac gtactgttga agctttgtac aaatttgtgg ttcctaagcc 3241 ccgaagtgaa tgtacaaagg cagagcagct ggttgtgaca tttgtggcag gttacatagc 3301 tggagtcttc tgtgcgatcg tctcccaccc tgctgactct gtggtctctg tgctgaataa 3361 agagaaaggc agcaccgcgt ctcaggtcct gcagaggctg ggcttcagag gtgtgtggaa 3421 ggggctcttc gcccgaatca tcatgattgg cactctgact gcactacagt ggttcatcta 3481 cgactctgtg aaggtctact tcaggctccc tcgccctcct ccccctgaga tgccagagtc 3541 tctgaagaag aagcttgggt taactgagTA gggatccgcg gccgctgggt aggggaggcg 3601 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac 3661 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg 3721 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc 3781 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc 3841 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa 3901 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc 3961 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca 4021 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc 4081 ctttcgacct gcagcccaag cttaccatgg agagcgacga gagcggcctg cccgccatgg 4141 agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg 4201 gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc 4261 tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca 4321 cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca 4381 acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct 4441 acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca 4501 gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca 4561 tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct 4621 actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc 4681 tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg 4741 agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc 4801 gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc 4861 gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga 4921 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca 4981 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc 5041 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg 5101 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg 5161 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc 5221 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg 5281 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg 5341 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga 5401 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg 5461 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtcg 5521 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 5581 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 5641 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 5701 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 5761 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 5821 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 5881 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc 5941 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 6001 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 6061 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct 6121 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 6181 ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg 6241 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 6301 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 6361 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 6421 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 6481 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 6541 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 6601 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc 6661 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 6721 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag 6781 acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 6841 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 6901 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 6961 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 7021 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 7081 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 7141 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 7201 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 7261 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 7321 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 7381 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 7441 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 7501 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 7561 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7621 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 7681 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 7741 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 7801 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 7861 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 7921 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 7981 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 8041 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 8101 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 8161 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 8221 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 8281 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 8341 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 8401 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 8461 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 8521 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 8581 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 8641 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 8701 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 8761 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 8821 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 8881 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 8941 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 9001 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac 9061 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 9121 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat 9181 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 9241 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 9301 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 9361 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 9421 ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.