pCMV-EGFP-RPTOR-3UTR-6×His-Pur

pCMV-EGFP-RPTOR-3UTR-6×His-Puro plasmid

PVT96880

pCMV-EGFP-RPTOR-3UTR-6×His-Puro plasmid Information

Plasmid name: pCMV-EGFP-RPTOR(human)-3UTR-6×His-Puro
Alias: NM_001163034.2;KOG1; Mip1
Gene lenght: 3534bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-RPTOR-3UTR-6×His-Puro plasmid Description

pCMV-EGFP-RPTOR-3UTR-6×His-Puro is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-RPTOR-3UTR-6×His-Puro plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGCC ACCatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag AAGCTTggtg gaggcggatc aggaggcggt 1621 ggatctGAAT TCatggagtc cgaaatgctg caatcgcctc ttctgggcct gggggaggaa 1681 gatgaggctg atcttacaga ctggaaccta cctttggctt ttatgaaaaa gaggcactgt 1741 gagaaaattg aaggctccaa atccttagct cagagctgga ggatgaagga tcggatgaag 1801 acagtcagtg ttgccttagt tttgtgcctg aatgttggtg tggaccctcc cgatgtggtg 1861 aagaccacgc cctgtgcacg cttggaatgc tggatcgatc ctctgtcgat gggtcctcag 1921 aaagctctgg aaaccatcgg tgcaaattta cagaagcagt acgagaactg gcagccaagg 1981 gcccggtaca agcagagcct tgacccaact gtggatgaag tcaagaagct ctgcacgtcc 2041 ttacgtcgca acgccaagga ggagcgagtc ctctttcact acaatggcca cggggtgccc 2101 cggcccacag tcaacgggga ggtctgggtc ttcaacaaga actacacgca gtacatccct 2161 ctgtccatat atgacctgca gacgtggatg ggcagcccgt cgatcttcgt ctacgactgc 2221 tccaatgctg gcttgatcgt caagtccttc aagcagttcg cactacagcg ggagcaggag 2281 ctggaggtag ctgcaatcaa cccaaatcac cctcttgctc agatgccttt gcctccgtcg 2341 atgaaaaact gcatccagct ggcagcctgc gaggccaccg agctgctgcc catgatcccc 2401 gacctcccgg ctgacctatt cacctcctgc ctcaccaccc ccatcaagat cgccctgcgc 2461 tggttttgca tgcagaaatg tgtcagtctg gtgcctggcg tcacactgga tttgatagaa 2521 aagatccctg gccgcctgaa cgacaggagg acgcccctgg gtgaactgaa ctggatcttc 2581 acagccatca cagacaccat cgcgtggaac gtgctccccc gggatctctt ccaaaagctc 2641 ttcagacagg acttgctggt ggctagtctg tttcgaaatt ttttattggc ggaaaggatt 2701 atgaggtcgt ataactgcac tcccgtcagc agcccgcgtc tgccgcccac gtacatgcac 2761 gccatgtggc aagcctggga cctggctgtt gacatctgtc tgtctcagct gccgacgatc 2821 atcgaggaag gcactgcgtt tcggcacagc ccgttcttcg ccgagcagct gaccgcattc 2881 caggtgtggc tcaccatggg cgtggagaac cgaaacccac ccgaacagct gcccatcgtc 2941 ctgcaggtgc tgttaagcca agtgcaccgg ctgagagcat tggacttgct tggaagattt 3001 ttggacctgg gtccctgggc agtgagcctg gccttgtctg tcggcatctt cccctacgtg 3061 ctgaagctgc tccagagctc ggcccgagag ctgcggccac ttctcgtttt catctgggcc 3121 aagatcctcg cagtggacag cgagctggtg gtggctctga gtcatcttgt ggttcagtat 3181 gaaagcaatt tctgcaccgt ggccctgcag ttcatagaag aggaaaagaa ctacgccttg 3241 ccttctccag caaccacaga gggagggagt ttgaccccag tgcgagacag cccgtgcacc 3301 cccagacttc gttctgtgag ctcctatgga aacatccgtg ctgtcgccac agccaggagc 3361 ctcaacaaat ctttgcagaa cctgagtttg acagaggaat ctggtggcgc ggtggcgttc 3421 tcccccggaa acctcagcac cagcagcagc gccagcagca ccctgggcag ccccgagaat 3481 gaggagcata tcctgtcctt cgagaccatc gacaagatgc gccgcgccag ctcctactcc 3541 tccctcaact ccctcatcgg agtttccttt aacagtgttt acactcagat ttggagagtc 3601 ctgctgcacc tggctgctga cccctatcca gaggtctcgg acgtggccat gaaagtactc 3661 aacagcatcg cctacaaggc caccgtgaac gcccggccgc agcgcgtcct ggacacctcc 3721 tccctcacgc agtcggcccc cgccagcccc accaacaagg gcgtgcacat ccaccaggcg 3781 gggggctccc ctccggcgtc cagcaccagc agctccagcc tgaccaacga tgtggccaag 3841 cagccggtca gccgagactt gccttctggc cggccgggca ccacaggccc cgctggggcg 3901 cagtacaccc ctcactccca ccagttcccc cggacacgga agatgttcga caagggccca 3961 gagcagactg cggacgacgc ggacgatgct gctggacaca aaagtttcat ctccgccacg 4021 gtgcagacgg ggttctgcga ctggagcgcc cgctattttg cccagcccgt catgaagatc 4081 ccagaagagc acgacctgga gagtcagatc cgcaaggagc gggagtggcg gttcctgcga 4141 aacagccgtg tcaggaggca ggcccagcaa gtcattcaga agggcattac gagattggac 4201 gaccaaatat ttctgaacag gaaccccggc gtcccctctg tggtgaaatt ccaccccttc 4261 acgccgtgca tcgccgtagc cgacaaggac agcatctgct tttgggactg ggagaaaggg 4321 gagaagctgg attatttcca caatgggaac cctcggtaca cgagggtcac tgccatggag 4381 tatctgaacg gccaggactg ctcgcttctg ctgacggcca cagacgatgg tgccatcagg 4441 gtctggaaga attttgctga tttggaaaag aacccagaga tggtgaccgc gtggcagggg 4501 ctctcggaca tgctgccaac gacgcgagga gctgggatgg tggtggactg ggagcaggag 4561 accggcctcc tcatgagctc aggagacgtg cggatcgtcc ggatctggga cacagaccgt 4621 gagatgaagg tgcaggacat ccctacgggc gcagacagct gtgtgacgag tctgtcctgt 4681 gattcccacc gctcactcat cgtggctggc ctcggtgacg gctccatccg cgtctacgac 4741 agaaggatgg cactcagcga atgccgcgtc atgacgtacc gggagcacac agcctgggtg 4801 gtgaaggcct ccctgcagaa gcgtcccgac ggccacatcg tgagtgtgag cgtcaatgga 4861 gatgtgcgca tctttgatcc ccggatgcct gagtcggtaa atgtgcttca gatcgtgaag 4921 gggctgacgg ccctggacat ccacccccag gcggacctga tcgcatgtgg ctccgtcaat 4981 cagttcaccg ccatctacaa cagcagcgga gagctcatca acaacatcaa gtactacgac 5041 ggcttcatgg gccagcgggt cggcgccatc agctgcctgg ccttccaccc gcactggcct 5101 cacctggccg tgggaagcaa cgactactac atctccgtgt actcggtgga gaagcgtgtc 5161 agaccctgct actcgctttt gtctgtcttc gctgtcgtgt ctggaatgtc agGGAAGGGG 5221 AGGGCTCGgg ttgacggtgg cttcccactg agcaccagcC ATCACCATCA TCACCATTAG 5281 CTCGAGTCTA GAGGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC 5341 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC 5401 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT 5461 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG 5521 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCT 5581 AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 5641 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 5701 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 5761 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 5821 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 5881 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 5941 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 6001 TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT 6061 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA 6121 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT 6181 AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT 6241 CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG 6301 CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT 6361 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG 6421 GATCGTTTCG Catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 6481 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 6541 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 6601 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga 6661 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg 6721 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 6781 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 6841 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc 6901 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 6961 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc 7021 ccggtgcctg aGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC 7081 ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT 7141 CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA 7201 CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT 7261 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT 7321 ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA 7381 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG 7441 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG 7501 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA 7561 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 7621 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 7681 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 7741 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 7801 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 7861 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 7921 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 7981 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 8041 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 8101 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 8161 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 8221 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 8281 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC 8341 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 8401 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 8461 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 8521 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 8581 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 8641 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 8701 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 8761 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 8821 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 8881 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 8941 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 9001 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 9061 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 9121 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 9181 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 9241 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 9301 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 9361 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 9421 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 9481 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG 9541 AGATCTCCCG ATCCCCTATG GT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.